Fer-1 (Ferrostatin-1)

Catalog No.S7243 Batch:S724301

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Technical Data

Formula

C15H22N2O2
Molecular Weight 262.35 CAS No. 347174-05-4
Solubility (25°C)* In vitro DMSO 52 mg/mL (198.2 mM)
Ethanol 52 mg/mL (198.2 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description Fer-1 (Ferrostatin-1) is a potent and selective inhibitor of ferroptosis with EC50 of 60 nM.
Targets
Ferroptosis [1]
(HT-1080 cells)
60 nM(EC50)
In vitro

Ferrostatin-1 (2 μM) prevents erastin-induced ferroptosis in cancer cells, as well as glutamate-induced cell death in postnatal rat brain slices. Ferrostatin-1 is a lipid ROS scavenger, with the N-cyclohexyl moiety serving as a lipo-philic anchor within biological membranes. Ferrostatin-1 does not inhibit extracellular signal -regulated kinase (ERK) phos-phorylation or arrest the proliferation of HT-1080 cells, suggesting that it does not inhibit the MEK/ERK pathway, chelate iron, or inhibit protein synthesis. Ferrostatin-1 does, however, prevent erastin-induced accumulation of cytosolic and lipid ROS. Ferrostatin-1 readily oxidizes the stable radical 2,2-diphenyl-1-picrylhydrazyl (DPPH) under cell-free conditions. [1]
In vivo

Ferrostatin-1 (Fer-1), a synthetic antioxidant, is a potent and selective inhibitor of ferroptosis. It acts via a reductive mechanism to prevent damage to membrane lipids and thereby inhibits cell death.[2]

Protocol (from reference)

Cell Assay:

[1]
  • Cell lines

    HT-1080 cells

  • Concentrations

    60 nM (EC50)

  • Incubation Time

    24 h

  • Method

    Erastin treated cells were incubated with ferrostatin-1 at 37˚C for 24 hr and viability was assessed using Alamar Blue assay.
Animal Study:

[2]
  • Animal Models

    C57BL/6 mice

  • Dosages

    10 mg/kg

  • Administration

    i.n.

Customer Product Validation

Data from [Data independently produced by , , Hepatology, 2016, 64(2):488-500]

Data from [Data independently produced by , , Cancer Res, 2017, 77(8):2064-2077]

Data from [Data independently produced by , , Oncogene, 2015, 10.1038/onc.2015.32]

Data from [Data independently produced by , , Mol Carcinog, 2018, 57(11):1566-1576]

Selleck's Fer-1 (Ferrostatin-1) has been cited by 506 publications

7-Dehydrocholesterol dictates ferroptosis sensitivity [ Nature, 2024, 626(7998):411-418.] PubMed: 38297130
7-Dehydrocholesterol dictates ferroptosis sensitivity [ Nature, 2024, 626(7998):411-418.] PubMed: 38297130
Characterization of ferroptosis-triggered pyroptotic signaling in heart failure [ Signal Transduct Target Ther, 2024, 9(1):257] PubMed: 39327446
CircPIAS1 promotes hepatocellular carcinoma progression by inhibiting ferroptosis via the miR-455-3p/NUPR1/FTH1 axis [ Mol Cancer, 2024, 23(1):113] PubMed: 38802795
Sensing steroid hormone 17α-hydroxypregnenolone by GPR56 enables protection from ferroptosis-induced liver injury [ Cell Metab, 2024, S1550-4131(24)00371-1] PubMed: 39389061
Integrative clinical and preclinical studies identify FerroTerminator1 as a potent therapeutic drug for MASH [ Cell Metab, 2024, S1550-4131(24)00284-5] PubMed: 39142286
METTL16-SENP3-LTF axis confers ferroptosis resistance and facilitates tumorigenesis in hepatocellular carcinoma [ J Hematol Oncol, 2024, 17(1):78] PubMed: 39218945
A CRISPRi/a screening platform to study cellular nutrient transport in diverse microenvironments [ Nat Cell Biol, 2024, 26(5):825-838] PubMed: 38605144
Methionine-SAM metabolism-dependent ubiquinone synthesis is crucial for ROS accumulation in ferroptosis induction [ Nat Commun, 2024, 15(1):8971] PubMed: 39420002
SLC13A3 is a major effector downstream of activated β-catenin in liver cancer pathogenesis [ Nat Commun, 2024, 15(1):7522] PubMed: 39215042

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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