Home Metabolism Ferroptosis activator iFSP1 For research use only.

iFSP1

iFSP1 is a potent, selective and glutathione-independent inhibitor of ferroptosis suppressor protein 1 (FSP1/AIFM2) with EC50 of 103 nM. iFSP1 selectively induces ferroptosis in GPX4-knockout Pfa1 and HT1080 cells that overexpresses FSP1.Solutions are unstable and should be fresh-prepared.

iFSP1 Chemical Structure

iFSP1 Chemical Structure

CAS No. 150651-39-1

Purity & Quality Control

Batch: S966301 DMSO]20 mg/mL]false]Water]Insoluble]false]Ethanol]Insoluble]false Purity: 99.90%
99.90

iFSP1 Related Products

Biological Activity

Description iFSP1 is a potent, selective and glutathione-independent inhibitor of ferroptosis suppressor protein 1 (FSP1/AIFM2) with EC50 of 103 nM. iFSP1 selectively induces ferroptosis in GPX4-knockout Pfa1 and HT1080 cells that overexpresses FSP1.Solutions are unstable and should be fresh-prepared.
Targets
FSP1/AIFM2 [1]
(Cell-free assay)
103 nM(EC50)
In vitro
In vitro

iFSP1 selectively induces ferroptosis in GPX4-knockout Pfa1 and HT1080 cells that overexpressed FSP1. FSP1 is expressed in most tumour cell lines, and iFSP1 treatment robustly sensitizes these cells to RSL3-induced ferroptosis.[1]
Cell Research Cell lines human cancer cell lines (NCl-H1437, U-373, MDA-MB-436, SW620, MDA-MB-435S, A549, etc.)
Concentrations 0.01 μM - 10 μM, 3 μM
Incubation Time 24 h
Method

Cells are seeded on 96-well plates (2,500 cells per well) and treated with iFSP-1 after plating. Cell viability is assessed 24 h (unless stated otherwise) after treatment using AquaBluer as an indicator of viable cells according to the manufacturer's recommendations.

Chemical Information & Solubility

Molecular Weight 323.35 Formula

C20H13N5
CAS No. 150651-39-1 SDF --
Smiles CC1=CC=C(C=C1)C2=C(C3=NC4=CC=CC=C4N3C(=C2C#N)N)C#N
Storage (From the date of receipt) 3 years -20°C powder Solutions are unstable. Prepare fresh or purchase small, pre-packaged sizes. Repackage upon receipt.

In vitro
Batch:

DMSO : 20 mg/mL ( (61.85 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
Batch:

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In vivo Formulation Calculator

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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