Home Apoptosis Ferroptosis inhibitor SRS16-86 For research use only.

SRS16-86

SRS16-86, a novel third-generation ferrostatin, is an inhibitor of ferroptosis.

SRS16-86 Chemical Structure

SRS16-86 Chemical Structure

CAS No. 1793052-96-6

Purity & Quality Control

Batch: S984001 DMSO]2 mg/mL]false]Ethanol]2 mg/mL]false]Water]Insoluble]false Purity: 98.73%
98.73

SRS16-86 Related Products

Biological Activity

Description SRS16-86, a novel third-generation ferrostatin, is an inhibitor of ferroptosis.
In vitro
In vitro

SRS16-86 exhibits ferroptosis-inhibiting ability in vitro by FACS analysis of erastin-treated HT-1080 cells and in NIH 3T3 cells.[1]
Cell Research Cell lines HT-1080 cells, NIH 3T3 cells
Concentrations 1 μM
Incubation Time 24 h, 40 h
Method

Phosphatidylserine exposure to the outer cell membrane of apoptotic cells or at the inner plasma membrane of necrotic cells and incorporation of 7-AAD into necrotic cells are quantified by fluorescence-activated cell sorting (FACS) analysis. FACS analysis is used for the necrotic marker 7AAD and phosphatidylserine accessibility (annexin V) in HT1080 and NIH 3T3 cells treated with either vehicle or 50 µM erastin in the presence or absence of 1 μM SRS16-86.
In Vivo
In vivo

SRS16-86 (a novel third-generation ferrostatin) is more stable, to metabolism and plasma, and more potent, compared with the first-in-class compound ferrostatin-1 (Fer-1) to suppress ferroptosis in vivo. Even in conditions with extraordinarily severe IRI, SRS16-86 exerts strong protection to an extent which has not previously allowed survival in any murine setting.[1]
Animal Research Animal Models 8- to 12-wk-old male C57BL/6 mice
Dosages 2 mg/kg
Administration IP

Chemical Information & Solubility

Molecular Weight 432.56 Formula

C26H32N4O2
CAS No. 1793052-96-6 SDF --
Smiles CC(C)(C)OC(=O)C1=CC(=C(NC23CC4CC(CC(C4)C2)C3)C=C1)N=CC5=CN=CN=C5
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 2 mg/mL ( (4.62 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 2 mg/mL

Water : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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