SRS16-86

Catalog No.S9840 Batch:S984001

Technical Data

Formula	C₂₆H₃₂N₄O₂
Molecular Weight	432.56	CAS No.	1793052-96-6
Solubility (25°C)*	In vitro	DMSO	2 mg/mL (4.62 mM)
		Ethanol	2 mg/mL (4.62 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	SRS16-86, a novel third-generation ferrostatin, is an inhibitor of ferroptosis.
In vitro	SRS16-86 exhibits ferroptosis-inhibiting ability in vitro by FACS analysis of erastin-treated HT-1080 cells and in NIH 3T3 cells.^[1]
In vivo	SRS16-86 (a novel third-generation ferrostatin) is more stable, to metabolism and plasma, and more potent, compared with the first-in-class compound ferrostatin-1 (Fer-1) to suppress ferroptosis in vivo. Even in conditions with extraordinarily severe IRI, SRS16-86 exerts strong protection to an extent which has not previously allowed survival in any murine setting.^[1]

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines HT-1080 cells, NIH 3T3 cells Concentrations 1 μM Incubation Time 24 h, 40 h Method Phosphatidylserine exposure to the outer cell membrane of apoptotic cells or at the inner plasma membrane of necrotic cells and incorporation of 7-AAD into necrotic cells are quantified by fluorescence-activated cell sorting (FACS) analysis. FACS analysis is used for the necrotic marker 7AAD and phosphatidylserine accessibility (annexin V) in HT1080 and NIH 3T3 cells treated with either vehicle or 50 µM erastin in the presence or absence of 1 μM SRS16-86.
Animal Study:^{^[1]}	Animal Models 8- to 12-wk-old male C57BL/6 mice Dosages 2 mg/kg Administration IP

Cell Assay:^{^[1]}

Cell lines
HT-1080 cells, NIH 3T3 cells
Concentrations
1 μM
Incubation Time
24 h, 40 h
Method
Phosphatidylserine exposure to the outer cell membrane of apoptotic cells or at the inner plasma membrane of necrotic cells and incorporation of 7-AAD into necrotic cells are quantified by fluorescence-activated cell sorting (FACS) analysis. FACS analysis is used for the necrotic marker 7AAD and phosphatidylserine accessibility (annexin V) in HT1080 and NIH 3T3 cells treated with either vehicle or 50 µM erastin in the presence or absence of 1 μM SRS16-86.

Animal Study:^{^[1]}

Animal Models
8- to 12-wk-old male C57BL/6 mice
Dosages
2 mg/kg
Administration
IP

References

[1] Andreas Linkermann, et al. Proc Natl Acad Sci U S A. 2014 Nov 25;111(47):16836-41.

Selleck's SRS16-86 has been cited by 1 publication

Trabectedin induces ferroptosis via regulation of HIF-1α/IRP1/TFR1 and Keap1/Nrf2/GPX4 axis in non-small cell lung cancer cells [ Chem Biol Interact, 2022, 369:110262]	PubMed: 36396105

Trabectedin induces ferroptosis via regulation of HIF-1α/IRP1/TFR1 and Keap1/Nrf2/GPX4 axis in non-small cell lung cancer cells [ Chem Biol Interact, 2022, 369:110262]

PubMed: 36396105

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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