Home Neuronal Signaling Adrenergic Receptor antagonist Esmolol For research use only.

Esmolol

Esmolol is a cardioselective beta1 receptor blocker with rapid onset, a very short duration of action, and no significant intrinsic sympathomimetic or membrane stabilising activity at therapeutic dosages.

Esmolol Chemical Structure

Esmolol Chemical Structure

CAS No. 81147-92-4

Purity & Quality Control

Batch: S577801 DMSO]59 mg/mL]false]Water]59 mg/mL]false]Ethanol]59 mg/mL]false Purity: 99.38%
99.38

Esmolol Related Products

Signaling Pathway

Adrenergic Receptor Signaling Pathways

Adrenergic Receptor Signaling Pathway

Biological Activity

Description Esmolol is a cardioselective beta1 receptor blocker with rapid onset, a very short duration of action, and no significant intrinsic sympathomimetic or membrane stabilising activity at therapeutic dosages.
Targets
Human β1-Receptor [1] Human β2-Receptor [1]
194 nM(Ki) 5.8 μM(Ki)
In vitro
In vitro Esmolol acts as a pharmacochaperone: Long term exposure of cells to esmolol raised the surface levels of β1-adrenergic receptors[1].
Cell Research Cell lines HEK293 cells expressing human β1- and β2-adrenergic receptors
Concentrations 1 μM
Incubation Time 40 min
Method

Cells are seeded into 6-cm dishes in DMEM containing 10% FCS, allowed to adhere for 8 hours, and then rendered quiescent by serum starvation for 16 hours. Cells are subsequently stimulated by addition of medium containing or lacking 1 μM esmolol, 1 μM landiolol, or 1 μM isoproterenol (as a positive control). Cells are maintained at 37°C for up to 40 minutes, the incubation is terminated by rapid rinsing with ice-cold PBS. Thereafter, the dish is immediately immersed in liquid nitrogen. After rapid thawing, cells were disrupted by addition of 80 μl of lysis buffer, 1% Nonidet P-40, 0.1% SDS, 250 IU/ml aprotinin, and 40 μg/ml leupeptin. The cellular debris was removed by centrifugation at 10,000g for 10 minutes, and the total protein content was measured photometrically using bicinchoninic acid.
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT06390748 Completed
Sepsis|Lymphocyte Disorder T|Immunologic Paralysis|Catecholamine; Overproduction|Beta-Blocker|Cytokine Storm|Sympathetic Nervous System Diseases
Lin Chen|Sichuan Provincial People''s Hospital
 January 1 2021 Phase 2
NCT04137991 Completed
Nociceptive Pain|Goal-directed Therapy|Hemodynamic Instability|Nol-Index|Remifentanil|Cardiac Surgery|Vascular Surgery|General Anesthesia
Erasme University Hospital
 October 10 2019 Not Applicable

Chemical Information & Solubility

Molecular Weight 295.37 Formula

C16H25NO4
CAS No. 81147-92-4 SDF --
Smiles CC(C)NCC(COC1=CC=C(C=C1)CCC(=O)OC)O
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 59 mg/mL ( (199.74 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 59 mg/mL

Ethanol : 59 mg/mL


Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

Mass Concentration Volume Molecular Weight

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy Esmolol | Esmolol ic50 | Esmolol price | Esmolol cost | Esmolol solubility dmso | Esmolol purchase | Esmolol manufacturer | Esmolol research buy | Esmolol order | Esmolol mouse | Esmolol chemical structure | Esmolol mw | Esmolol molecular weight | Esmolol datasheet | Esmolol supplier | Esmolol in vitro | Esmolol cell line | Esmolol concentration | Esmolol nmr