Home Immunology & Inflammation ROS modulator Ensulizole For research use only.

Ensulizole

Synonyms: PBSA

Ensulizole (PBSA), a water soluble sunscreen ingredient, is a sulfonated UV absorber which is characterized by intense UVB and partial UVA absorption. Ensulizole can damage the DNA through the generation of reactive oxygen species (ROS) upon UV or sunlight irradiation.

Ensulizole Chemical Structure

Ensulizole Chemical Structure

CAS No. 27503-81-7

Purity & Quality Control

Batch: S441901 DMSO]5 mg/mL]false]Water]Insoluble]false]Ethanol]Insoluble]false Purity: 98.95%
98.95

Ensulizole Related Products

Biological Activity

Description Ensulizole (PBSA), a water soluble sunscreen ingredient, is a sulfonated UV absorber which is characterized by intense UVB and partial UVA absorption. Ensulizole can damage the DNA through the generation of reactive oxygen species (ROS) upon UV or sunlight irradiation.
Targets
ROS [1]
In vitro
In vitro

Ensulizole selectively inhibits activity of HDAC3.[2]
Cell Research Cell lines HCT116, HEK293, HepG2, SU-DHL-6 cell lines
Concentrations --
Incubation Time --
Method

HCT116, HEK293, HepG2, and SU-DHL-6 cells were seeded into 1536-well white solid bottom assay plates at desired cell densities in 5 μL medium using a microfluidic reagent dispenser. Before plating 5 μL of human neural stem cells into each well, assay plates were pre-coated with 5 μL of 10 μg/mL fibronectin in culture medium at 37°C for one hour and the coating solution was removed by centrifuging the inverted plates. Cells were settled overnight in a cell incubator at 37°C, 5% CO2, followed by addition of 23 nL of compound solution on a pintool workstation. After compound incubation for indicated period at 37°C/5% CO2, 5 μL of HDAC-Glo was dispensed using BioRAPTR FRD. To measure cell viability, 5 μL of CellTiter-Glo Cell Viability reagent was added to each well in a separate assay plate using BioRAPTR FRD. The luminescence signals of HDAC-Glo and CellTiter-Glo assays were measured on a ViewLux plate reader.



(Data sourced from selleck products)

Chemical Information & Solubility

Molecular Weight 274.30 Formula

C13H10N2O3S
CAS No. 27503-81-7 SDF --
Smiles O[S](=O)(=O)C1=CC2=C(C=C1)N=C([NH]2)C3=CC=CC=C3
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 5 mg/mL ( (18.22 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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