Ensulizole

Catalog No.S4419 Batch:S441901

Technical Data

Formula	C₁₃H₁₀N₂O₃S
Molecular Weight	274.30	CAS No.	27503-81-7
Solubility (25°C)*	In vitro	DMSO	5 mg/mL (18.22 mM)
		Water	Insoluble
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

Ensulizole (PBSA), a water soluble sunscreen ingredient, is a sulfonated UV absorber which is characterized by intense UVB and partial UVA absorption. Ensulizole can damage the DNA through the generation of reactive oxygen species (ROS) upon UV or sunlight irradiation.

Targets

ROS ^[1]

In vitro

Ensulizole selectively inhibits activity of HDAC3.^[2]

Protocol (from reference)

Cell Assay:^{^[2]}	Cell lines HCT116, HEK293, HepG2, SU-DHL-6 cell lines Concentrations -- Incubation Time -- Method HCT116, HEK293, HepG2, and SU-DHL-6 cells were seeded into 1536-well white solid bottom assay plates at desired cell densities in 5 μL medium using a microfluidic reagent dispenser. Before plating 5 μL of human neural stem cells into each well, assay plates were pre-coated with 5 μL of 10 μg/mL fibronectin in culture medium at 37°C for one hour and the coating solution was removed by centrifuging the inverted plates. Cells were settled overnight in a cell incubator at 37°C, 5% CO₂, followed by addition of 23 nL of compound solution on a pintool workstation. After compound incubation for indicated period at 37°C/5% CO₂, 5 μL of HDAC-Glo was dispensed using BioRAPTR FRD. To measure cell viability, 5 μL of CellTiter-Glo Cell Viability reagent was added to each well in a separate assay plate using BioRAPTR FRD. The luminescence signals of HDAC-Glo and CellTiter-Glo assays were measured on a ViewLux plate reader. (Data sourced from selleck products)

Cell Assay:^{^[2]}

Cell lines
HCT116, HEK293, HepG2, SU-DHL-6 cell lines
Concentrations
--
Incubation Time
--
Method
HCT116, HEK293, HepG2, and SU-DHL-6 cells were seeded into 1536-well white solid bottom assay plates at desired cell densities in 5 μL medium using a microfluidic reagent dispenser. Before plating 5 μL of human neural stem cells into each well, assay plates were pre-coated with 5 μL of 10 μg/mL fibronectin in culture medium at 37°C for one hour and the coating solution was removed by centrifuging the inverted plates. Cells were settled overnight in a cell incubator at 37°C, 5% CO₂, followed by addition of 23 nL of compound solution on a pintool workstation. After compound incubation for indicated period at 37°C/5% CO₂, 5 μL of HDAC-Glo was dispensed using BioRAPTR FRD. To measure cell viability, 5 μL of CellTiter-Glo Cell Viability reagent was added to each well in a separate assay plate using BioRAPTR FRD. The luminescence signals of HDAC-Glo and CellTiter-Glo assays were measured on a ViewLux plate reader.

(Data sourced from selleck products)

References

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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