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CY5

Synonyms: Sulfo-Cyanine5

CY5 (Sulfo-Cyanine5) is a far-red fluorescent dye widely used in molecular biology for labeling nucleic acids, proteins, and other biomolecules. With excitation and emission peaks at approximately 649 nm and 670 nm, respectively, it is particularly suited for fluorescence-based applications such as Förster Resonance Energy Transfer (FRET). In single-molecule RNA studies, Cy5 is frequently attached to a biotinylated DNA tether, functioning as an acceptor dye to track RNA folding dynamics, molecular interactions, and enzymatic activity with high precision.

CY5 Chemical Structure

CY5 Chemical Structure

CAS No. 146368-11-8

Purity & Quality Control

Batch: E187301 DMSO]100 mg/mL]false]Water]25 mg/mL]false]Ethanol]8 mg/mL]false Purity: 99.77%
99.77

CY5 Related Products

Biological Activity

Description CY5 (Sulfo-Cyanine5) is a far-red fluorescent dye widely used in molecular biology for labeling nucleic acids, proteins, and other biomolecules. With excitation and emission peaks at approximately 649 nm and 670 nm, respectively, it is particularly suited for fluorescence-based applications such as Förster Resonance Energy Transfer (FRET). In single-molecule RNA studies, Cy5 is frequently attached to a biotinylated DNA tether, functioning as an acceptor dye to track RNA folding dynamics, molecular interactions, and enzymatic activity with high precision.

Chemical Information & Solubility

Molecular Weight 656.81 Formula

C33H40N2O8S2
CAS No. 146368-11-8 SDF Download CY5 SDF
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 100 mg/mL ( (152.25 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 25 mg/mL

Ethanol : 8 mg/mL


Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

Preparing Stock Solutions

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In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

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Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
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Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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