Adalimumab (anti-TNF-alpha)

Synonyms: LU200134, D2E7

Catalog No.A2010 Purity:99.23%

Adalimumab (anti-TNF-alpha) is the first fully human, recombinant IgG1 monoclonal antibody that specifically targets human TNF-alpha, MW: 144.19 KD.

Adalimumab (anti-TNF-alpha) Chemical Structure

Click to purchase the isotype control of Adalimumab (anti-TNF-alpha)

Selleck's Adalimumab (anti-TNF-alpha) has been cited by 10 publications

Purity & Quality Control

Batch: Purity: 99.23% Protein concentration: 5.00 mg/ml Endotoxin Level: ≤1 EU/mg

99.23

Choose Selective TNF-alpha Inhibitors

Name	Citation	TNF-α	Others
Necrostatin-1	261	+
QNZ (EVP4593)	138	++++	NF-κB
GSK2982772	4	++
GSK481	✔

Expand to Check More

1. "+" indicates inhibitory effect. Increased inhibition is marked by a higher "+" designation. 2. "✔" indicates inhibitory effect, but without specific value.

Biological Activity

Description

Adalimumab (anti-TNF-alpha) is the first fully human, recombinant IgG1 monoclonal antibody that specifically targets human TNF-alpha, MW: 144.19 KD.

Features

Human IgG1

Targets

TNF-α ^[1]

In Vitro
In vitro	Adalimumab treatment alters the secretion of cytokines.^[1]
Cell Research:	Fa2N-4 cells were co-cultivated (CC) with monocyte derived macrophages (MDMs) for 24 h. Physiological (15%) and pro-inflammatory (50%) MDM-to-Fa2N-4 ratios were used. All measured cytokine concentrations were related to the corresponding control (fold change) to analyse (A) the IL-8 neutralising antibody (αIL-8, 100 ng/ml) specific effect and (B) the TNF-α neutralising effect of Adalimumab.

In Vitro

In vitro

Adalimumab treatment alters the secretion of cytokines.^[1]

Cell Research:

Fa2N-4 cells were co-cultivated (CC) with monocyte derived macrophages (MDMs) for 24 h. Physiological (15%) and pro-inflammatory (50%) MDM-to-Fa2N-4 ratios were used. All measured cytokine concentrations were related to the corresponding control (fold change) to analyse (A) the IL-8 neutralising antibody (αIL-8, 100 ng/ml) specific effect and (B) the TNF-α neutralising effect of Adalimumab.

In Vivo
In vivo	We injected adalimumab intraductally into the MG of adult mice;this was found to stimulate BSC multipotency.^[2]
Animal Research	Animal Models: K5CreER, K8rtTA, TetO-DTA mice Dosages: 0.15 mg Administration: i.d. Reference:^[2]

References
[1] Padberg F, et al. 2021 Jun;73:105134. [2] Centonze A, et al. Nature. 2020 Aug;584(7822):608-613.

References

Cell Lines	Assay Type	Concentration	Incubation Time	Formulation	Activity Description	PMID

Product Details

CAS No.	331731-18-1
Isotype	Human IgG1
Source	CHO cells
Formulation	100 mM Pro, 20 mM Arg, pH 5.0
Storage (From the date of receipt)	Store the undiluted solution at 4°C in the dark to avoid freeze-thaw cycles

Comparison of Clones for

^*Literature analysis of various clone (for this target) products available on the market shows that Selleck's selected clones are more frequently applied. (data until September 2024)

Tech Support

If you have any other enquiries, please leave a message.

* Indicates a Required Field

* Name Please enter your name.

* Email Please enter your email. Please enter a valid email address.

Phone

* Comments: Please write something to us.

Frequently Asked Questions

Question 1:
How to store the antibody?

Answer:
Store the undiluted solution at 4 °C in the dark. Freezing antibodies can result in a loss of activity caused by the freezing/thawing process. Diluting antibodies to working concentrations and storing at 4°C for more than a day should be avoided. Additionally, make sure to keep the antibody sterile. Under these storage conditions, your antibodies should remain active for up to one year and oftentimes longer.