Adalimumab (anti-TNF-alpha)

Synonyms: LU200134, D2E7

Catalog No.A2010 Purity:99.23%

Adalimumab (anti-TNF-alpha) is the first fully human, recombinant IgG1 monoclonal antibody that specifically targets human TNF-alpha, MW: 144.19 KD.

Adalimumab (anti-TNF-alpha) Chemical Structure

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Selleck's Adalimumab (anti-TNF-alpha) has been cited by 10 publications

Purity & Quality Control

Batch: Purity: 99.23% Protein concentration: 5.00 mg/ml Endotoxin Level: ≤1 EU/mg

99.23

Choose Selective TNF-alpha Inhibitors

Name	Citation	TNF-α	Others
Necrostatin-1	254	+
QNZ (EVP4593)	136	++++	NF-κB
GSK2982772	4	++
GSK481	✔

Expand to Check More

1. "+" indicates inhibitory effect. Increased inhibition is marked by a higher "+" designation. 2. "✔" indicates inhibitory effect, but without specific value.

Biological Activity

Description

Adalimumab (anti-TNF-alpha) is the first fully human, recombinant IgG1 monoclonal antibody that specifically targets human TNF-alpha, MW: 144.19 KD.

Features

Human IgG1

Targets

TNF-α ^[1]

In Vitro
In vitro	Adalimumab treatment alters the secretion of cytokines.^[1]
Cell Research:	Fa2N-4 cells were co-cultivated (CC) with monocyte derived macrophages (MDMs) for 24 h. Physiological (15%) and pro-inflammatory (50%) MDM-to-Fa2N-4 ratios were used. All measured cytokine concentrations were related to the corresponding control (fold change) to analyse (A) the IL-8 neutralising antibody (αIL-8, 100 ng/ml) specific effect and (B) the TNF-α neutralising effect of Adalimumab.

In Vitro

In vitro

Adalimumab treatment alters the secretion of cytokines.^[1]

Cell Research:

Fa2N-4 cells were co-cultivated (CC) with monocyte derived macrophages (MDMs) for 24 h. Physiological (15%) and pro-inflammatory (50%) MDM-to-Fa2N-4 ratios were used. All measured cytokine concentrations were related to the corresponding control (fold change) to analyse (A) the IL-8 neutralising antibody (αIL-8, 100 ng/ml) specific effect and (B) the TNF-α neutralising effect of Adalimumab.

In Vivo
In vivo	We injected adalimumab intraductally into the MG of adult mice;this was found to stimulate BSC multipotency.^[2]
Animal Research	Animal Models: K5CreER, K8rtTA, TetO-DTA mice Dosages: 0.15 mg Administration: i.d. Reference:^[2]

References
[1] Padberg F, et al. 2021 Jun;73:105134. [2] Centonze A, et al. Nature. 2020 Aug;584(7822):608-613.

References

Cell Lines	Assay Type	Concentration	Incubation Time	Formulation	Activity Description	PMID

Product Details

CAS No.	331731-18-1
Isotype	Human IgG1
Source	CHO cells
Formulation	100 mM Pro, 20 mM Arg, pH 5.0
Storage (From the date of receipt)	Store the undiluted solution at 4°C in the dark to avoid freeze-thaw cycles

Comparison of Clones for

^*Literature analysis of various clone (for this target) products available on the market shows that Selleck's selected clones are more frequently applied. (data until September 2024)

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Frequently Asked Questions

Question 1:
How to store the antibody?

Answer:
Store the undiluted solution at 4 °C in the dark. Freezing antibodies can result in a loss of activity caused by the freezing/thawing process. Diluting antibodies to working concentrations and storing at 4°C for more than a day should be avoided. Additionally, make sure to keep the antibody sterile. Under these storage conditions, your antibodies should remain active for up to one year and oftentimes longer.