Home Apoptosis TNF-alpha inhibitor Benpyrine racemate For research use only.

Benpyrine racemate

Synonyms: (Rac)-Benpyrine

Benpyrine racemate ((Rac)-Benpyrine) is a potent, highly specific and orally active inhibitor of TNF-α and attenuates TNF-α-induced inflammation, thereby reducing liver and lung injury.

Benpyrine racemate Chemical Structure

Benpyrine racemate Chemical Structure

CAS No. 1333714-43-4

Purity & Quality Control

Batch: S351501 DMSO]31 mg/mL]false]Water]Insoluble]false]Ethanol]Insoluble]false Purity: 99.49%
99.49

Benpyrine racemate Related Products

Signaling Pathway

TNF-alpha Signaling Pathways

TNF-alpha Signaling Pathway

Biological Activity

Description Benpyrine racemate ((Rac)-Benpyrine) is a potent, highly specific and orally active inhibitor of TNF-α and attenuates TNF-α-induced inflammation, thereby reducing liver and lung injury.
Targets
TNF-α [1]
In vitro
In vitro

Benpyrine directly binds to TNF-α and blocks TNF-α-trigged signaling activation.[1]
Cell Research Cell lines RAW264.7 macrophages
Concentrations 5, 10 or 20 μM
Incubation Time 12 h
Method

RAW 264.7 cells were grown to 50−70% confluence in a glass chamber, pretreated with DMSO or benpyrine for 12 h, and then stimulated with 10 ng/mL TNF-α for 2 h. Cell treatments were terminated by washing the cells with PBS, followed by fixation with freshly prepared 4% paraformaldehyde in PBS for 20 min. The fixed cells were washed three times with PBS and then permeabilized with 0.25% Triton X-100 in PBS for 10 min. After blocking with 2 mg/mL BSA for 1 h at room temperature, we added the NF-κB/p65 antibody at a 1:1000 dilution at 4 °C overnight, washed three times with PBS, then incubated with a 1:500 dilution of the secondary antibody for 1 h at room temperature in the dark. Finally, 4′,6-diamidino-2-phenyl indole (DAPI) was used to stain the nuclei at 37 °C for 30 min in the dark.
In Vivo
In vivo

Benpyrine attenuates TNF-α-induced inflammation in endotoxemic murine model, thereby reducing liver and lung injury, and significantly reliefs the symptoms of collagen-induced arthritis and imiquimod-induced psoriasiform inflammation in mice.[1]
Animal Research Animal Models CIA mice, Imiquimod-Induced Psoriasis-like Skin Lesions mice mode
Dosages 25, 50 mg/kg
Administration i.g.

Chemical Information & Solubility

Molecular Weight 308.34 Formula

C16H16N6O
CAS No. 1333714-43-4 SDF --
Smiles O=C1CC(CN1CC2=CC=CC=C2)NC3=NC=NC4=C3[NH]C=N4
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 31 mg/mL ( (100.53 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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