Home Endocrinology & Hormones Estrogen/progestogen Receptor agonist Ethinyl Estradiol For research use only.

Ethinyl Estradiol

Ethinyl Estradiol is an orally bio-active estrogen used in almost all modern formulations of combined oral contraceptive pills.

Ethinyl Estradiol Chemical Structure

Ethinyl Estradiol Chemical Structure

CAS No. 57-63-6

Purity & Quality Control

Ethinyl Estradiol Related Products

Signaling Pathway

Estrogen/progestogen Receptor Signaling Pathways

Estrogen/progestogen Receptor Signaling Pathway

Biological Activity

Description Ethinyl Estradiol is an orally bio-active estrogen used in almost all modern formulations of combined oral contraceptive pills.
Targets
Estrogen receptor [1]
In vitro
In vitro Ethinyl Estradiol increases respiratory chain activity in both cultured rat hepatocytesand HepG2 cells. Ethinyl estradiol is a strong promoter of hepatocarcinogenesis. [1] Ethinyl Estradiol enhances the transcript levels of nuclear genome- and mitochondrial genome-encoded genes and respiratory chain activity in female rat liver, and also inhibits transforming growth factor beta (TGFbeta)-induced apoptosis in cultured liver slices and hepatocytes from female rats. Ethinyl Estradiol increases the transcript levels of the mitochondrial genome-encoded genes cytochrome oxidase subunits I, II, and III in cultured female rathepatocytes. Ethinyl Estradiol significantly increases both the levels of glutathione (reduced [GSH] and oxidized [GSSG] forms) per mg protein in mitochondria and nuclei, while the percentage of total glutathione in the oxidized form is not affected. [2]
In Vivo
In vivo Ethinyl Estradiol (50 mg/kg/day) increases anogenital distance and reduces pup body weight at postnatal day 2, accelerates the age at vaginal opening, reduces F1 fertility and F2 litter sizes, and induces malformations of the external genitalia (5 mg/kg) in the female Long-Evans rat. [3] Ethinyl Estradiol increases the number of low density lipoprotein (LDL) receptors in livers of rats, thereby producing a profound fall in plasma cholesterol levels. Ethinyl Estradiol exerts the same effect in livers of male and female rabbits and that the increase in receptor number is correlated with a 6- to 8-fold increase in the levels of receptor mRNA. [4]
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT06380205 Recruiting
Healthy Participants
Incyte Corporation
 May 7 2024 Phase 1
NCT06334315 Not yet recruiting
Contraception|Pharmacogenomic Drug Interaction
Yale University|Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD)
 May 2024 Phase 4
NCT06039826 Active not recruiting
Overweight
Eli Lilly and Company
 September 12 2023 Phase 1
NCT05671653 Terminated
Obesity
Pfizer
 January 19 2023 Phase 1
NCT05360550 Completed
Pregnancy Prevention
Lupin Research Inc
 July 5 2022 Phase 2

Chemical Information & Solubility

Molecular Weight 296.4 Formula

C20H24O2
CAS No. 57-63-6 SDF Download Ethinyl Estradiol SDF
Smiles CC12CCC3C(C1CCC2(C#C)O)CCC4=C3C=CC(=C4)O
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 59 mg/mL ( (199.05 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 59 mg/mL

Water : Insoluble


Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

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Mass Concentration Volume Molecular Weight

In vivo Formulation Calculator (Clear solution)

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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