Home Others Dyes chemical 3,3'-Diaminobenzidine tetrahydrochloride For research use only.

3,3'-Diaminobenzidine tetrahydrochloride

Synonyms: DAB

3,3'-Diaminobenzidine tetrahydrochloride (DAB) is the most sensitive and commonly used substrate for horseradish peroxidase, which can visualize the color of cells or tissues bound by horseradish peroxidase during immunohistochemistry or in situ hybridization.

3,3'-Diaminobenzidine tetrahydrochloride Chemical Structure

3,3'-Diaminobenzidine tetrahydrochloride Chemical Structure

CAS No. 868272-85-9

Purity & Quality Control

Batch: E269001 DMSO]54 mg/mL]false]Water]54 mg/mL]false]Ethanol]Insoluble]false Purity: 99.51%
99.51

3,3'-Diaminobenzidine tetrahydrochloride Related Products

Biological Activity

Description 3,3'-Diaminobenzidine tetrahydrochloride (DAB) is the most sensitive and commonly used substrate for horseradish peroxidase, which can visualize the color of cells or tissues bound by horseradish peroxidase during immunohistochemistry or in situ hybridization.
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Chemical Information & Solubility

Molecular Weight 378.1254 Formula

C12H14N4.4ClH.H2O
CAS No. 868272-85-9 SDF --
Smiles O.Cl.Cl.Cl.Cl.NC1=C(N)C=C(C=C1)C2=CC(=C(N)C=C2)N
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 54 mg/mL ( (142.8 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 54 mg/mL

Ethanol : Insoluble


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In vivo
Batch:

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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