Home Epigenetics Histone Acetyltransferase inhibitor - Apoptosis related activator WM-3835 For research use only.

WM-3835

WM-3835 is a novel and high-specific small molecule Lysine Acetyltransferase 7 (KAT7, MYST2, HBO1) inhibitor, able to potently suppressed OS cell proliferation and migration, and leads to apoptosis activation.

WM-3835 Chemical Structure

WM-3835 Chemical Structure

CAS No. 2229025-70-9

Purity & Quality Control

Batch: S980501 DMSO]80 mg/mL]false]Ethanol]80 mg/mL]false]Water]Insoluble]false Purity: 98.30%
98.30

WM-3835 Related Products

Signaling Pathway

Histone Acetyltransferase Signaling Pathways

Histone Acetyltransferase Signaling Pathway

Biological Activity

Description WM-3835 is a novel and high-specific small molecule Lysine Acetyltransferase 7 (KAT7, MYST2, HBO1) inhibitor, able to potently suppressed OS cell proliferation and migration, and leads to apoptosis activation.
Targets
KAT7 [1]
In vitro
In vitro

WM-3835 activates apoptosis while inhibites OS cell proliferation, migration and invasion. The activity of WM-3835 is nullified in HBO1-KO OS cells. Also, the HBO1 inhibitor is non-cytotoxic in HBO1-low human osteoblasts, which indicates that the anti-OS cell efficacy of WM-3835 is primarily through HBO1 inhibition.[1]
Cell Research Cell lines Primary human OS cells (pOS-1 cells, pOS-2 cells)
Concentrations 1 μM, 5 μM, 10 μM, 25 μM
Incubation Time 24 h, 48 h, 72 h, 96 h
Method

The pOS-1 cells are treated with WM-3835 or vehicle control (“Veh”, 0.5% DMSO), cells with treatments are seeded into 96-well plates at 3×103 cells per well. After 96 h incubation, in each well 10 μL CCK-8 reagent is added for 3 h. Later, CCK-8's OD value is recorded at 450 nm. Cell death is tested by Trypan blue staining using an automatic cell counter.
In Vivo
In vivo

In vivo, intraperitoneal injection of a single dose of WM-3835 potently inhibites OS xenograft growth in SCID mice.[1]
Animal Research Animal Models SCID mice
Dosages 10 mg/kg
Administration IP

Chemical Information & Solubility

Molecular Weight 400.42 Formula

C20H17FN2O4S
CAS No. 2229025-70-9 SDF --
Smiles CC1=CC(=CC(=C1F)C(=O)NN[S](=O)(=O)C2=CC(=CC=C2)O)C3=CC=CC=C3
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 80 mg/mL ( (199.79 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 80 mg/mL

Water : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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