WM-3835

Catalog No.S9805 Batch:S980501

Technical Data

Formula

C₂₀H₁₇FN₂O₄S

Molecular Weight

400.42

CAS No.

2229025-70-9

Solubility (25°C)*

In vitro

DMSO

80 mg/mL (199.79 mM)

Ethanol

80 mg/mL (199.79 mM)

Water

Insoluble

In vivo (Add solvents to the product individually and in order)

Clear solution

10% DMSO 1 40%PEG300 2 5%Tween80 3 45%ddH2O

9.0mg/ml

Taking the 1 mL working solution as an example, add 100 μL of 90 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 450 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

WM-3835 is a novel and high-specific small molecule Lysine Acetyltransferase 7 (KAT7, MYST2, HBO1) inhibitor, able to potently suppressed OS cell proliferation and migration, and leads to apoptosis activation.

Targets

KAT7 ^[1]

In vitro

WM-3835 activates apoptosis while inhibites OS cell proliferation, migration and invasion. The activity of WM-3835 is nullified in HBO1-KO OS cells. Also, the HBO1 inhibitor is non-cytotoxic in HBO1-low human osteoblasts, which indicates that the anti-OS cell efficacy of WM-3835 is primarily through HBO1 inhibition.^[1]

In vivo

In vivo, intraperitoneal injection of a single dose of WM-3835 potently inhibites OS xenograft growth in SCID mice.^[1]

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines Primary human OS cells (pOS-1 cells, pOS-2 cells) Concentrations 1 μM, 5 μM, 10 μM, 25 μM Incubation Time 24 h, 48 h, 72 h, 96 h Method The pOS-1 cells are treated with WM-3835 or vehicle control (“Veh”, 0.5% DMSO), cells with treatments are seeded into 96-well plates at 3×10³ cells per well. After 96 h incubation, in each well 10 μL CCK-8 reagent is added for 3 h. Later, CCK-8's OD value is recorded at 450 nm. Cell death is tested by Trypan blue staining using an automatic cell counter.
Animal Study:^{^[1]}	Animal Models SCID mice Dosages 10 mg/kg Administration IP

Cell Assay:^{^[1]}

Cell lines
Primary human OS cells (pOS-1 cells, pOS-2 cells)
Concentrations
1 μM, 5 μM, 10 μM, 25 μM
Incubation Time
24 h, 48 h, 72 h, 96 h
Method
The pOS-1 cells are treated with WM-3835 or vehicle control (“Veh”, 0.5% DMSO), cells with treatments are seeded into 96-well plates at 3×10³ cells per well. After 96 h incubation, in each well 10 μL CCK-8 reagent is added for 3 h. Later, CCK-8's OD value is recorded at 450 nm. Cell death is tested by Trypan blue staining using an automatic cell counter.

Animal Study:^{^[1]}

Animal Models
SCID mice
Dosages
10 mg/kg
Administration
IP

References

[1] Yan-Yang Gao, et al. Theranostics. 2021 Mar 4;11(10):4599-4615.

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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