Home Microbiology Bacterial chemical Trigonelline Hydrochloride For research use only.

Trigonelline Hydrochloride

Synonyms: Nicotinic acid N-methylbetaine Hydrochloride, Coffearine Hydrochloride, Gynesine Hydrochloride

Trigonelline (Nicotinic acid N-methylbetaine, Coffearine, Gynesine), a major alkaloid component of fenugreek, has hypoglycemic, hypolipidemic, neuroprotective, antimigraine, sedative, memory-improving, antibacterial, antiviral, and anti-tumor activities, and also has been shown to reduce diabetic auditory neuropathy and platelet aggregation.

Trigonelline Hydrochloride Chemical Structure

Trigonelline Hydrochloride Chemical Structure

CAS No. 6138-41-6

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Trigonelline Hydrochloride Related Products

Biological Activity

Description Trigonelline (Nicotinic acid N-methylbetaine, Coffearine, Gynesine), a major alkaloid component of fenugreek, has hypoglycemic, hypolipidemic, neuroprotective, antimigraine, sedative, memory-improving, antibacterial, antiviral, and anti-tumor activities, and also has been shown to reduce diabetic auditory neuropathy and platelet aggregation.
In vitro
In vitro Trigonelline suppresses lipid droplet accumulation in a concentration (75 and 100 μM) dependent manner. Treatment of adipocyte with of Trigonelline down-regulates the peroxisome proliferator-activated receptor (PPAR) and CCAAT element binding protein (C/EBP- ) mRNA expression, which leads to further down regulation of other gene such as adiponectin, adipogenin, leptin, resistin andadipocyte fatty acid binding protein (aP2) as compared with respective control cells on 5th and 10thday of differentiation [2].
Cell Research Cell lines 3T3-L1 cell
Concentrations 25, 50, 75 and 100 μM
Incubation Time 24 and 48 h
Method The 3T3-L1 pre-adipocytes cells are seeded in the 96 well at a density of 1 × 105cells/well. The cells are treated with different concentrations (25, 50, 75 and 100 μM) of Trigonelline. It is incubated at the 37◦C in 5% CO2 incubator for 24 and 48 h and then the culture is treated with WST reagent incubated for 2-4 h. The living cells absorb the WST then it is converted into an orange color product. Then, the intensity of color is measured at 450 nmusing spectra count ELISA reader.
In Vivo
In vivo Trigonelline has been shown to reduce diabetic auditory neuropathy and platelet aggregation. In rabbits, the main parameters for the fenugreek extract (i.g.) are as follows: T1/2(Ka)=0.9 h, T1/2(Ke)=2.2 h, V=0.64 L/kg, area under the curve=1.93 mg/min/L. The main parameters for trigonelline (i.v.) are as follows: T1/2α=10.8 min, T1/2β=44.0 min, K21=0.044/min, K10=0.026/min, K12=0.017/min, area under the concentration-time curve=931.0 mg/min/L. Trigonelline has a moderate rate of absorption and high rate of elimination in the rabbit. Trigonelline has beneficial effects in several human diseases, notably diabetes and central nervous system disease. Its properties include hypoglycemic, hypolipidemic, improving diabetic auditory neuropathy, neuroprotective, antimigraine, sedative, antibacterial, antiviral, and anti-tumor activities; it also improves memory retention and inhibits platelet aggregation[1].

Chemical Information & Solubility

Molecular Weight 173.6 Formula

C7H8NO2.Cl
CAS No. 6138-41-6 SDF Download Trigonelline Hydrochloride SDF
Smiles C[N+]1=CC=CC(=C1)C(=O)O.[Cl-]
Storage (From the date of receipt)

In vitro
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DMSO : 18 mg/mL ( (103.68 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)


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