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Berberine

Synonyms: Umbellatine

Berberine (Umbellatine), an alkaloid isolated from Rhizoma coptidis, has broad applications, particularly as an antibacterial agent in the clinic.

Berberine Chemical Structure

Berberine Chemical Structure

CAS No. 2086-83-1

Purity & Quality Control

Berberine Related Products

Biological Activity

Description Berberine (Umbellatine), an alkaloid isolated from Rhizoma coptidis, has broad applications, particularly as an antibacterial agent in the clinic.
In vitro
In vitro

In 3T3-L1 adipocytes, the treatment of 12 μM or 20 μM berberine combined with 25 μM resveratrol shows more significant inhibition of lipid accumulation compared with individual compounds. The combination of berberine with resveratrol significantly increases the low-density-lipoprotein receptor expression in HepG2 cells to a level about one-fold higher in comparison to individual compounds.[1]
Cell Research Cell lines 3T3-L1 adipocytes, HepG2 cells, hepatic L02 cells
Concentrations 12 μM, 20 μM, 50 μM
Incubation Time 3 days, 24 h
Method

At day 10 of differentiation, 3T3-L1 adipocytes are treated with Berberine, resveratrol, and their combination for 3 days. The intracellular lipid accumulation of differentiated cells is detected by oil red O staining. HepG2 cells are cultured in 6-well plate for 24 h with a 3 × 105 cell density, and then the culture medium is replaced by fresh medium containing Berberine, resveratrol, and their combination for another 24 h. Subsequently, the total proteins from the cells are extracted for assaying the low-density-lipoprotein receptor (LDLR) expression by Western Blot.
In Vivo
In vivo

The combination of Berberine and resveratrol exerts a significant enhanced effect on lipid-lowering in hyperlipidemic mice and intracellular lipid accumulation-decreasing in adipocytes as compared to individual agent alone, which is closely associated with SIRT1 and LDLR.[1]
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT06273241 Not yet recruiting
Pharmacokinetic Study in Healthy Volunteers
University Medicine Greifswald
 March 4 2024 Not Applicable
NCT05845931 Recruiting
Pharmacokinetic Study in Healthy Volunteers
University Medicine Greifswald
 May 5 2023 Not Applicable
NCT05480670 Completed
Polycystic Ovary Syndrome
Ayub Teaching Hospital
 November 1 2022 Not Applicable
NCT05463003 Completed
Pharmacokinetic Study in Healthy Volunteers
University Medicine Greifswald
 July 19 2022 Not Applicable

Chemical Information & Solubility

Molecular Weight 336.36 Formula

C20H18NO4
CAS No. 2086-83-1 SDF --
Smiles COC1=C(C2=C[N+]3=C(C=C2C=C1)C4=CC5=C(C=C4CC3)OCO5)OC
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 22.5 mg/mL ( (66.89 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 5 mg/mL

Water : 1 mg/mL


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In vivo
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