Berberine

Catalog No.S9046 Batch:S904601

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Technical Data

Formula

C20H18NO4
Molecular Weight 336.36 CAS No. 2086-83-1
Solubility (25°C)* In vitro DMSO 22.5 mg/mL (66.89 mM)
Ethanol 5 mg/mL (14.86 mM)
Water 1 mg/mL (2.97 mM)
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description Berberine (Umbellatine), an alkaloid isolated from Rhizoma coptidis, has broad applications, particularly as an antibacterial agent in the clinic.
In vitro

In 3T3-L1 adipocytes, the treatment of 12 μM or 20 μM berberine combined with 25 μM resveratrol shows more significant inhibition of lipid accumulation compared with individual compounds. The combination of berberine with resveratrol significantly increases the low-density-lipoprotein receptor expression in HepG2 cells to a level about one-fold higher in comparison to individual compounds.[1]
In vivo

The combination of Berberine and resveratrol exerts a significant enhanced effect on lipid-lowering in hyperlipidemic mice and intracellular lipid accumulation-decreasing in adipocytes as compared to individual agent alone, which is closely associated with SIRT1 and LDLR.[1]

Protocol (from reference)

Cell Assay:

[1]
  • Cell lines

    3T3-L1 adipocytes, HepG2 cells, hepatic L02 cells

  • Concentrations

    12 μM, 20 μM, 50 μM

  • Incubation Time

    3 days, 24 h

  • Method

    At day 10 of differentiation, 3T3-L1 adipocytes are treated with Berberine, resveratrol, and their combination for 3 days. The intracellular lipid accumulation of differentiated cells is detected by oil red O staining. HepG2 cells are cultured in 6-well plate for 24 h with a 3 × 105 cell density, and then the culture medium is replaced by fresh medium containing Berberine, resveratrol, and their combination for another 24 h. Subsequently, the total proteins from the cells are extracted for assaying the low-density-lipoprotein receptor (LDLR) expression by Western Blot.

Selleck's Berberine has been cited by 12 publications

The improvement of modified Si-Miao granule on hepatic insulin resistance and glycogen synthesis in type 2 diabetes mellitus involves the inhibition of TNF-α/JNK1/IRS-2 pathway: network pharmacology, molecular docking, and experimental validation [ Chin Med, 2024, 19(1):128.] PubMed: 39285464
System analysis of Huang-Lian-Jie-Du-Tang and their key active ingredients for overcoming CML resistance by suppression of leukemia stem cells [ Phytomedicine, 2023, 117:154918] PubMed: 37329755
Nao Tan Qing ameliorates Alzheimer's disease-like pathology by regulating glycolipid metabolism and neuroinflammation: A network pharmacology analysis and biological validation [ Pharmacol Res, 2022, 185:106489] PubMed: 36228869
Vagus nerve stimulated by microbiota-derived hydrogen sulfide mediates the regulation of berberine on microglia in transient middle cerebral artery occlusion rats [ Phytother Res, 2022, 10.1002/ptr.7490] PubMed: 35583808
Berberine Sensitizes Human Hepatoma Cells to Regorafenib via Modulating Expression of Circular RNAs [ Front Pharmacol, 2021, 12:632201] PubMed: 34220494
Quantitative Proteomics Analysis of Berberine-Treated Colon Cancer Cells Reveals Potential Therapy Targets [ Biology (Basel), 2021, 10(3)250] PubMed: 33806918
Berberine exerts neuroprotective activities against cerebral ischemia/reperfusion injury through up-regulating PPAR-γ to suppress NF-κB-mediated pyroptosis [ Brain Res Bull, 2021, 177:22-30] PubMed: 34517069
Sensitivity of pancreatic cancer cells to chemotherapeutic drugs, signal transduction inhibitors and nutraceuticals can be regulated by WT-TP53 [ Adv Biol Regul, 2021, S2212-4926(20)30091-9] PubMed: 33451973
Suppression effect of plant-derived berberine on cyprinid herpesvirus 2 proliferation and its pharmacokinetics in Crucian carp (Carassius auratus gibelio) [ Antiviral Res, 2020, 186:105000] PubMed: 33359191
Berberine Inhibits Nod-Like Receptor Family Pyrin Domain Containing 3 Inflammasome Activation and Pyroptosis in Nonalcoholic Steatohepatitis via the ROS/TXNIP Axis. [ Front Pharmacol, 2020, 11:185] PubMed: 32194416

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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