Home JAK/STAT STAT inhibitor Ochromycinone (STA-21) For research use only.

Ochromycinone (STA-21)

Ochromycinone (STA-21) is a selective STAT3 inhibitor.

Ochromycinone (STA-21) Chemical Structure

Ochromycinone (STA-21) Chemical Structure

CAS No. 111540-00-2

Purity & Quality Control

Ochromycinone (STA-21) Related Products

Signaling Pathway

STAT Signaling Pathways

STAT Signaling Pathway

Biological Activity

Description Ochromycinone (STA-21) is a selective STAT3 inhibitor.
Targets
STAT3 [1]
In vitro
In vitro In cells, STA-21 inhibits Stat3 DNA binding activity, Stat3 dimerization, and Stat3-dependent luciferase activity. STA-21 remarkably inhibits the growth and the survival of the breast carcinoma cells MDA-MB-231, MDA-MB-435s, and MDA-MB-468 that express persistently activated Stat3. [1] In RH30 and RD2 cells, STA-21 also inhibits cell viability and growth and induced apoptosis through caspases 3, 8 and 9 pathways. [2]
Cell Research Cell lines MDA-MB-231, MDA-MB-435s, MDA-MB-453, MDA-MB-468, MCF7, and Caov-3 cells
Concentrations 20 or 30 μM
Incubation Time 48 h
Method Human breast cancer cell lines MDA-MB-231, MDA-MB-435s, MDA-MB-453, MDA-MB-468, and MCF7, human ovarian carcinoma cell line Caov-3, and human skin fibroblasts were cultured in DMEM containing 10% FBS and appropriate antibiotics in a 5% CO2 incubator at 37°C. For testing different compounds, the cells were exposed to the compounds for 48 h at a final concentration of 20 or 30 μM, respectively. Then, the cells were harvested and subjected to flow-cytometry analysis.
In Vivo
In vivo In IL-1Ra–KO mice, STA-21 (0.5 mg/kg, i.p.) reduces the arthritis score and the incidence of arthritis by decreasing the proportion of Th17 cells and increasing the proportion of Treg cells expressing FoxP3. [3]
Animal Research Animal Models IL-1Ra–KO mice
Dosages 0.5 mg/kg
Administration i.p.

Chemical Information & Solubility

Molecular Weight 306.31 Formula

C19H14O4
CAS No. 111540-00-2 SDF Download Ochromycinone (STA-21) SDF
Smiles CC1CC2=C(C(=O)C1)C3=C(C=C2)C(=O)C4=C(C3=O)C=CC=C4O
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 21 mg/mL ( (68.55 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 2 mg/mL

Water : Insoluble


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In vivo
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In vivo Formulation Calculator

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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