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Pinocembrin

Synonyms: Dihydrochrysin, Galangin flavanone, 5,7-Dihydroxyflavanone

Pinocembrin (Dihydrochrysin, Galangin flavanone, 5,7-Dihydroxyflavanone) is a major flavonoid molecule incorporated as multifunctional in the pharmaceutical industry. Its vast range of pharmacological activities has been well researched including antimicrobial, anti-inflammatory, antioxidant, and anticancer activities.

Pinocembrin Chemical Structure

Pinocembrin Chemical Structure

CAS No. 480-39-7

Purity & Quality Control

Batch: S394101 DMSO]51 mg/mL]false]Ethanol]51 mg/mL]false]Water]Insoluble]false Purity: 99.75%
99.75

Pinocembrin Related Products

Biological Activity

Description Pinocembrin (Dihydrochrysin, Galangin flavanone, 5,7-Dihydroxyflavanone) is a major flavonoid molecule incorporated as multifunctional in the pharmaceutical industry. Its vast range of pharmacological activities has been well researched including antimicrobial, anti-inflammatory, antioxidant, and anticancer activities.
In vitro
In vitro Pinocembrin (5,7-dihydroxyflavanone) is one of the primary flavonoids isolated from the variety of plants, mainly from Pinus heartwood, Eucalyptus, Populus, Euphorbia, and Sparattosperma leucanthum, in the diverse flora and purified by various chromatographic techniques. Its vast range of pharmacological activities has been well researched including antimicrobial, anti-inflammatory, antioxidant, and anticancer activities[1]. Pinocembrin has been shown to increase neuronal viability, decrease lactate dehydrogenase release, inhibit the production of NO and ROS, increase glutathione levels, and downregulate the expression of neuronal NO synthase (nNOS) and iNOS in primary cortical neurons subjected to oxygen-glucose deprivation/reoxygenation (OGD/R)[2].
Cell Research Cell lines SH-SY5Y cells
Concentrations 4, 8, 20 μM
Incubation Time 1 h
Method

SH-SY5Y cells are maintained in DMEM containing 10% FBS, 100 units/mL penicillin, and 100 µg/mL streptomycin in a humidified atmosphere containing 5% CO2 at 37℃.  Cells are passaged by trypsinization every 3-5 days. Then cells are seeded at 1 × 104 cells per well into a 96-well plate and then incubated for 24 h at 37 °C in a humidified atmosphere containing 5% CO2. The entire medium is then replaced with fresh medium containing 1% FBS, and one of the compounds is pretreated for 1 h, followed by the addition of 2.0 µg/mL tunicamycin or 30 nM staurosporine. Nuclear staining assays are carried out after a further 24 h of incubation. Cell death is assessed.
In Vivo
In vivo Pinocembrin can be used as neuroprotective against cerebral ischemic injury with a wide therapeutic time window, which may be attributed to its antiexcitotoxic effects[1]. It has the ability to reduce reactive oxygen species (ROS), protect the BBB, modulate mitochondrial function, and regulate apoptosis. Pinocembrin (10 mg/kg, i.v.) could reduce brain swelling; improve behavioral deficits; and alleviate neuronal apoptosis, edema of astrocytic end-feet, and the deformation of endothelial cells and capillaries. Pinocembrin reduces endoplasmic reticulum (ER) stress and apoptosis by decreasing C/EBP homologous protein (CHOP)/GADD153 and caspase-12 expression via the PERK-elF2α-ATF4 signaling pathway in MCAO rats. LD50 of i.v. pinocembrin in mice is greater than 700 mg/kg[2].
Animal Research Animal Models male Sprague-Dawley rats
Dosages 22.5 or 67.5 mg/kg
Administration i.v.

Chemical Information & Solubility

Molecular Weight 256.25 Formula

C15H12O4
CAS No. 480-39-7 SDF Download Pinocembrin SDF
Smiles C1C(OC2=CC(=CC(=C2C1=O)O)O)C3=CC=CC=C3
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 51 mg/mL ( (199.02 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 51 mg/mL

Water : Insoluble


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In vivo
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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