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Tetrahydropalmatine hydrochloride

Synonyms: Gindarine hydrochloride, 1-Tetrahydropalmitine HCl

Tetrahydropalmatine (THP, Gindarine, 1-Tetrahydropalmitine) is an isoquinoline alkaloid found in several different plant species, mainly in the Corydalis genus (Yan Hu Suo), but also in other plants such as Stephania rotunda. It is a potent muscle relaxant. Tetrahydropalmatine hydrochloride acts through inhibition of amygdaloid release of dopamine to inhibit an epileptic attack in rats.

Tetrahydropalmatine hydrochloride Chemical Structure

Tetrahydropalmatine hydrochloride Chemical Structure

CAS No. 6024-85-7

Purity & Quality Control

Batch: S385401 DMSO]12 mg/mL]false]]]false]]]false Purity: 99.74%
99.74

Tetrahydropalmatine hydrochloride Related Products

Biological Activity

Description Tetrahydropalmatine (THP, Gindarine, 1-Tetrahydropalmitine) is an isoquinoline alkaloid found in several different plant species, mainly in the Corydalis genus (Yan Hu Suo), but also in other plants such as Stephania rotunda. It is a potent muscle relaxant. Tetrahydropalmatine hydrochloride acts through inhibition of amygdaloid release of dopamine to inhibit an epileptic attack in rats.
In vitro
In vitro Tetrahydropalmatine inhibits the LPSinduced production of COX-2 protein and mRNA in a dose-dependent manner. It suppresses activation of the NF-kB signaling pathway, which results in regulation of IL-8 expression and specifically blocks the ERK1=2 and p38 MAPK signaling pathways[1]. L-THP results in p53 independent apoptosis via down-regulating XIAP protein by inhibiting MDM2 associated with proteasome-dependent pathway and increased sensitivity of EU-4 cells against doxorubicin. L-THP causes activation of caspase and resulted in apoptosis[2]. l-THP possesses a blocking effect on dopamine D1 and D2 receptors and voltage-sensitive Ca2+ channels[3].
Cell Research Cell lines THP-1 cells
Concentrations 0.2, 1, or 2 mM
Incubation Time 24 h
Method Cell viability is examined by MTT assay. In brief, THP-1 cells are seeded at a density of 5×104 cells/mL in 96-well plates. Each batch of cells includes a nontreated group as a control. Tetrahydropalmatine (0.2, 1, or 2 mM) and LPS (2 mg/mL) are then added to each well, after which the plates are incubated for 24 hours at 37℃ under 5% CO2. Next, MTT solutions (5 mg/mL) are added to each well, and the samples are then cultured for an additional 4 hours. The supernatant is then discarded, and 100 mL of dimethylsulfoxide is added to each well. Next, the optical density is read at 590 nm.
In Vivo
In vivo l-THP could attenuate the locomotor-stimulating effects of oxycodone and inhibit the development and expression of oxycodone behavioral sensitization[3].
Animal Research Animal Models Kunming mice
Dosages 6.25, 12.5, and 18.75 mg/kg
Administration administered intragastrically

Chemical Information & Solubility

Molecular Weight 391.89 Formula

C21H25NO4.HCl
CAS No. 6024-85-7 SDF --
Smiles COC1=C(C2=C(CC3C4=CC(=C(C=C4CCN3C2)OC)OC)C=C1)OC.Cl
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 12 mg/mL ( (30.62 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)


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