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Nortriptyline hydrochloride

Synonyms: Desitriptyline HCl, ELF-101 hydrochloride, EN-7048 hydrochloride, Desmethylamitriptyline hydrochloride

Nortriptyline hydrochloride (Desitriptyline, ELF-101, EN-7048, Desmethylamitriptyline) is the hydrochloride salt form of nortriptyline, a tricyclic antidepressant agent used for short-term treatment of various forms of depression.

Nortriptyline hydrochloride Chemical Structure

Nortriptyline hydrochloride Chemical Structure

CAS No. 894-71-3

Purity & Quality Control

Batch: S369801 DMSO]59 mg/mL]false]Water]59 mg/mL]false]Ethanol]59 mg/mL]false Purity: 99.97%
99.97

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Biological Activity

Description Nortriptyline hydrochloride (Desitriptyline, ELF-101, EN-7048, Desmethylamitriptyline) is the hydrochloride salt form of nortriptyline, a tricyclic antidepressant agent used for short-term treatment of various forms of depression.
In vitro
In vitro Nortriptyline inhibits the vascular Kv channels in a concentration-dependent and state-independent manner independently of serotonin reuptake inhibition[1]. It is a tricyclic antidepressant. At the cellular level, nortriptyline is inhibitory to many proteins and responses such as astroglial inwardly rectifying Kir4.1 channels, human neutrophil phagocytosis and oxidative burst, human cytochrome P-450 enzymes, opioid receptors, Ca2+-activated K+ channels, and priming of human neutrophils. Nortriptyline induces a significant [Ca2+]i rise and decreases viability in PC3 cells, MG63 human osteosarcoma, and renal tubular cells[2]. Nortriptyline also exhibits anticancer activity in several different types of cells. For example,in human cutaneous melanoma cells,NTP has a half maximal inhibitory concentration(IC50) of 9 μM compared with 27 μM and 33 μM for clomipramine and amitriptyline, respectively. Nortriptyline induces cell cycle arrest and apoptosis in TCCSUP and MBT-2 cells[3].
Cell Research Cell lines PC3 cells
Concentrations 50-500 µM
Incubation Time 24 h
Method Measurement of cell viability is based on the ability of cells to cleave tetrazolium salts by dehydrogenases. Augmentation in the amount of developed color directly correlates with the number of live cells. Cells are seeded in 96-well plates at 10,000 cells/well in culture medium for 24 h in the presence of several concentrations of nortriptyline. The cell viability-detecting reagent WST-1 is added to samples after nortriptyline treatment, and cells are incubated for 30 min in a humidified atmosphere.
In Vivo
In vivo Nortriptyline inhibits tumor growth in mice inoculated with MBT-2 cells. Low concentrations of NTP (0.1-5 μM) confer neuroprotective effects, downregulate cytosolic phospholipase A2, and prevent mitochondrial depolarization with minimal toxicity in both astrocytes and mice. In contrast, higher concentrations of NTP (10-50 μM) have anti-tumor effects on human osteosarcoma and cutaneous melanoma cells[3].
Animal Research Animal Models male C3H/HeN mice
Dosages 10 or 20 mg/kg
Administration i.p.

Chemical Information & Solubility

Molecular Weight 299.84 Formula

C19H21N.HCl
CAS No. 894-71-3 SDF Download Nortriptyline hydrochloride SDF
Smiles CNCCC=C1C2=CC=CC=C2CCC3=CC=CC=C31.Cl
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 59 mg/mL ( (196.77 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 59 mg/mL

Ethanol : 59 mg/mL


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