Home ER stress & UPR ROS activator MCB-613 For research use only.

MCB-613

MCB-613 is a potent steroid receptor coactivator (SRC) stimulator.

MCB-613 Chemical Structure

MCB-613 Chemical Structure

CAS No. 1162656-22-5

Purity & Quality Control

Batch: S791301 DMSO]60 mg/mL]false]Ethanol]60 mg/mL]false]Water]Insoluble]false Purity: 99.97%
99.97

MCB-613 Related Products

Biological Activity

Description MCB-613 is a potent steroid receptor coactivator (SRC) stimulator.
Targets
SRC [1]
In vitro
In vitro MCB-613 selectively and reversibly binds to the RID of SRC-3, and selectively kills cancer cells including MCF-7 (breast), PC-3 (prostate), H1299 (lung), and HepG2 (liver) cells, without toxicity to mouse primary hepatocytes and mouse embryonic fibroblasts (MEFs). MCB-613 also increases SRCs’ interactions with other coactivators and markedly induces ER stress coupled to the generation of reactive oxygen species (ROS). [1]
Kinase Assay Luciferase assays
After various compound treatments, cells are lysed in luciferase lysis buffer and assayed for luciferase activity using the ONE-Glo luciferase assay system. All luciferase activities are normalized to protein concentration determined by Bradford assay.
Cell Research Cell lines MCF-7 (breast), PC-3 (prostate), H1299 (lung), and HepG2 (liver) cells; mouse primary hepatocytes and mouse embryonic fibroblasts
Concentrations ~7 μM
Incubation Time 48 h
Method Cells are seeded in 96-well plates and allowed to reach 60% to 70% confluence. After indicated compound treatments, relative numbers of viable cells are measured by MTS assay using the Cell Titer 96 Aqueous One Solution Cell Proliferation Assay.
In Vivo
In vivo In an MCF-7 breast cancer mouse xenograft model, MCB-613 (20 mg/kg, i.p.) significantly and dramatically inhibits the growth of the tumor while causing no obvious animal toxicity and body weight less. [1]
Animal Research Animal Models Mice bearing MCF-7 xenografts
Dosages 20 mg/kg
Administration i.p.

Chemical Information & Solubility

Molecular Weight 304.39 Formula

C20H20N2O
CAS No. 1162656-22-5 SDF Download MCB-613 SDF
Smiles CCC1CC(=CC2=CN=CC=C2)C(=O)C(=CC3=CN=CC=C3)C1
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 60 mg/mL ( (197.11 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 60 mg/mL

Water : Insoluble


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In vivo
Batch:

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In vivo Formulation Calculator

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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