Home Epigenetics Histone Acetyltransferase activator CTB For research use only.

CTB

CTB is a p300 activator and the lowest docked energy for CTB and p300 is 7.72 kcal/mol.

CTB Chemical Structure

CTB Chemical Structure

CAS No. 451491-47-7

Purity & Quality Control

CTB Related Products

Signaling Pathway

Histone Acetyltransferase Signaling Pathways

Histone Acetyltransferase Signaling Pathway

Biological Activity

Description CTB is a p300 activator and the lowest docked energy for CTB and p300 is 7.72 kcal/mol.
In vitro
In vitro

CTB effectively induces apoptosis, up-regulates the expression of P300, and increases the acetylation and total P53 protein levels in MCF-7 cells.[2]
Cell Research Cell lines MCF-7 and MRC-5 cells
Concentrations 85.43 µM
Incubation Time 24, 48, 72 h
Method

MCF-7 and MRC-5 are cultured in RPMI-1640 and treated with or without CTB at a concentration of 85.43 μmol/L at different times (24, 48 and 72 h). The percentage of apoptotic cells are measured by flow cytometry.Real-time quantitative RT-PCR is performed to estimate the mRNA expression of P300 in MCF-7 and MRC-5 with CTB at different times. ELISA and Bradford protein techniques are used to detect levels of total and acetylated P53 protein generated in MCF-7 and MRC-5.
In Vivo
In vivo

CTB combinated with CpG, and aluminum hydroxide playsa synergistic effect to enhance ClfA221-550-induced immune responses, which possesses the potential to develop a novel vaccine to prevent infection of S. aureus.[3]
Animal Research Animal Models Specific-pathogen-free BALB/c mice
Dosages 100 μg
Administration i.m.
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT05433181 Not yet recruiting
SARS-CoV-2
University of Pennsylvania
 February 2025 Phase 1|Phase 2
NCT06344559 Not yet recruiting
Healthy
Rutgers The State University of New Jersey
 May 1 2024 Not Applicable
NCT06347055 Not yet recruiting
Nicotine Dependence
University of Missouri-Columbia
 April 15 2024 Not Applicable
NCT06301334 Not yet recruiting
Pain Mangement of Thoracotomy
Sohag University
 March 1 2024 Not Applicable
NCT05646732 Recruiting
Post Traumatic Stress Disorder|PTSD
Allyson Rosen|National Institute of Mental Health (NIMH)|Palo Alto Veterans Institute for Research
 August 2 2023 Not Applicable
NCT05983211 Recruiting
Amyotrophic Lateral Sclerosis
Sunnybrook Health Sciences Centre
 June 1 2023 Not Applicable

Chemical Information & Solubility

Molecular Weight 343.73 Formula

C16H13ClF3NO2
CAS No. 451491-47-7 SDF --
Smiles CCOC1=CC=CC=C1C(=O)NC2=CC=C(Cl)C(=C2)C(F)(F)F
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 69 mg/mL ( (200.73 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 17 mg/mL

Water : Insoluble


Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

Mass Concentration Volume Molecular Weight

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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