CTB

Catalog No.E1107 Batch:E110701

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Technical Data

Formula

C16H13ClF3NO2
Molecular Weight 343.73 CAS No. 451491-47-7
Solubility (25°C)* In vitro DMSO 69 mg/mL (200.73 mM)
Ethanol 17 mg/mL (49.45 mM)
Water Insoluble
In vivo (Add solvents to the product individually and in order)
Clear solution
5% DMSO 95% Corn oil
3.5mg/ml Taking the 1 mL working solution as an example, add 50 μL of 70 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O
3.5mg/ml Taking the 1 mL working solution as an example, add 50 μL of 70 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description CTB is a p300 activator and the lowest docked energy for CTB and p300 is 7.72 kcal/mol.
In vitro

CTB effectively induces apoptosis, up-regulates the expression of P300, and increases the acetylation and total P53 protein levels in MCF-7 cells.[2]
In vivo

CTB combinated with CpG, and aluminum hydroxide playsa synergistic effect to enhance ClfA221-550-induced immune responses, which possesses the potential to develop a novel vaccine to prevent infection of S. aureus.[3]

Protocol (from reference)

Cell Assay:

[2]
  • Cell lines

    MCF-7 and MRC-5 cells

  • Concentrations

    85.43 µM

  • Incubation Time

    24, 48, 72 h

  • Method

    MCF-7 and MRC-5 are cultured in RPMI-1640 and treated with or without CTB at a concentration of 85.43 μmol/L at different times (24, 48 and 72 h). The percentage of apoptotic cells are measured by flow cytometry.Real-time quantitative RT-PCR is performed to estimate the mRNA expression of P300 in MCF-7 and MRC-5 with CTB at different times. ELISA and Bradford protein techniques are used to detect levels of total and acetylated P53 protein generated in MCF-7 and MRC-5.
Animal Study:

[3]
  • Animal Models

    Specific-pathogen-free BALB/c mice

  • Dosages

    100 μg

  • Administration

    i.m.

RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.

SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.