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Baohuoside I

Synonyms: Icariside II

Baohuoside I (Icariside II) is a flavonoid isolated from Epimedium koreanum Nakai with anti-inflammatory and anti-cancer activities. It may exert cytotoxic effect via the ROS/MAPK pathway.

Baohuoside I Chemical Structure

Baohuoside I Chemical Structure

CAS No. 113558-15-9

Purity & Quality Control

Batch: S384001 DMSO]100 mg/mL]false]]]false]]]false Purity: 99.96%
99.96

Baohuoside I Related Products

Biological Activity

Description Baohuoside I (Icariside II) is a flavonoid isolated from Epimedium koreanum Nakai with anti-inflammatory and anti-cancer activities. It may exert cytotoxic effect via the ROS/MAPK pathway.
In vitro
In vitro Baohuoside I accelerates apoptosis through the mitochondrial apoptotic pathway, involving the increment of BAX/Bcl-2 ratio, dissipation of mitochondrial membrane potential, transposition of cytochrome c, caspase 3 and caspase 9 activation, degradation of poly (ADP-ribose) polymerase and the over-production of reactive oxygen species (ROS)[1]. In vitro studies with three leukaemia and three solid tumour cell lines show that Baohuoside I has cytotoxic properties, with IC50 values of between 2.8 and 7.5 μg/ml[2].
Cell Research Cell lines A549 cell
Concentrations 6.25, 12.5, and 25 μM
Incubation Time 24 h
Method Cells (1×106 cells/ml) are exposed to Baohuoside I (6.25, 12.5, and 25 μM) for 24 h, washed with PBS, and then harvested with trypsin EDTA, counted, fixed with 75% ethanol and stored at 4℃ prior to DNA content analyses. Samples are resuspended in 1.0 ml hypotonic propidium iodide (PI)-solution (50 mg/ml PI, 0.1% sodium citrate, 0.1% Triton X-100, 0.1 mg/ml ribonuclease A) and incubated in the dark for 1 h. PI-stained samples are analyzed with a flow cytometer.
In Vivo
In vivo Baohuoside I is both anti-inflammatory and immunosuppressive, but unlike the cytotoxic and anti-rejection drugs it suppresses only B-cell (antibody) and not T-cell responses. Baohuoside I may have a role in therapy of those autoimmune diseases[2].
Animal Research Animal Models Inbred female C57/BL/6J mice
Dosages 20, 40, 60 mg/kg
Administration i.p.
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT02931305 Unknown status
Osteoporosis|Cardiovascular Disease
National University Hospital Singapore
 October 2016 Phase 1

Chemical Information & Solubility

Molecular Weight 514.52 Formula

C27H30O10
CAS No. 113558-15-9 SDF Download Baohuoside I SDF
Smiles CC1C(C(C(C(O1)OC2=C(OC3=C(C(=CC(=C3C2=O)O)O)CC=C(C)C)C4=CC=C(C=C4)OC)O)O)O
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 100 mg/mL ( (194.35 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)


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In vivo
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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