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Anisomycin

Synonyms: Flagecidin, Wuningmeisu C

Anisomycin (Flagecidin, Wuningmeisu C) is a bacterial antibiotic isolated from Streptomyces griseolus, which inhibits protein synthesis, and also act as a JNK activator. Anisomycin upregulates autophagy and increases apoptosis.

Anisomycin Chemical Structure

Anisomycin Chemical Structure

CAS No. 22862-76-6

Purity & Quality Control

Anisomycin Related Products

Signaling Pathway

JNK Signaling Pathways

JNK Signaling Pathway

Cell Data

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HEK293 Function assay Inhibitory concentration required to produce cytotoxicity against HEK293 cells, IC50=0.02μM. 16005213
HeLa Function assay 10 uM Inhibition of translation in human HeLa cells at 10 uM by 35S-methionine metabolic labeling study 15165136
HEK293 Function assay 100 uM 15 mins Increase of JNK phosphorylation in U50488 treated untransfected HEK293 cells at 100 uM after 15 mins 17702750
HEK293 Function assay 50 uM 15 mins Increase of U50488-induced JNK phosphorylation in untransfected HEK293 cells at 50 uM after 15 mins 17702750
RAW264.7 Function assay 5 uM 30 mins Activation of p38MAPK in mouse RAW264.7 cells assessed as phosphorylation at Thr180/Tyr182 at 5 uM after 30 mins by Western blotting analysis 23294286
Sf9 Function assay 10 uM 6 to 24 hr Increase of ATP level in Spodoptera frugiperda (fall armyworm) Sf9 cells at 10 uM after 6 to 24 hr by luminescent cell viability assay ChEMBL
Sf9 Cytotoxicity assay 10 uM 72 hr Cytotoxicity against Spodoptera frugiperda (fall armyworm) Sf9 cells at 10 uM after 72 hr by trypan blue dye exclusion test ChEMBL
VERO-E6 Function assay 48 hrs Determination of IC50 values for inhibition of SARS-CoV-2 induced cytotoxicity of VERO-E6 cells after 48 hours exposure to 0.01 MOI SARS CoV-2 virus by high content imaging, IC50=0.09μM. ChEMBL
VERO-E6 Function assay 48 hrs Toxicity CC50 against VERO-E6 cells determined at 48 hours by high content imaging (same conditions as 2_LEY without exposure to 0.01 MOI SARS CoV-2 virus), CC50=0.1μM. ChEMBL
Vero E6 Function assay CC50 determination at MOI 0.004 using CellTiter- Glo (CTG) assay, performed 3 days post-infection in Vero E6 cells, CC50<0.39μM. ChEMBL
Vero E6 Function assay CC50 determination at MOI 0.01 using CellTiter- Glo (CTG) assay, performed 3 days post-infection in Vero E6 cells, CC50<0.39μM. ChEMBL
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Biological Activity

Description Anisomycin (Flagecidin, Wuningmeisu C) is a bacterial antibiotic isolated from Streptomyces griseolus, which inhibits protein synthesis, and also act as a JNK activator. Anisomycin upregulates autophagy and increases apoptosis.
Targets
JNK [1]
In vitro
In vitro Anisomycin (3 μM) decreases protein synthesis in MDA16 and MDA-MB-468 cells, and reduces colony formation by MDA-MB-468 cells. Anisomycin causes an increase in the number of apoptotic cells in MDA-MB-468 cultures, but not in MDA16 cultures. Anisomycin actives JNK phosphorylation in MDA-MB-468 cells.[2] In U251 and U87 cells, anisomycin (0.01-8 μM) inhibits the cell growth in time- and concentration-dependent manners with the IC50 (48 h) values of 0.233 and 0.192 μmol/L, respectively. Anisomycin (4 μM) causes 21.5% and 25.3% of apoptosis proportion in U251 and U87 cells, respectively, and activates p38 MAPK and JNK, while inactivated ERK1/2. Anisomycin (4 μM) reduces the level of PP2A/C subunit in a time-dependent manner in U251 and U87 cells.[3] Anisomycin inhibits EAC cell proliferation in concentration-dependent manner.[4]
Kinase Assay JNK phosphorylation
500,000 cells/well are seeded in 6-well plates and incubated overnight. Cells are then incubated for 1 h with test compounds or DMSO as vehicle control (final concentration 1% v/v). Puromycin is added (final concentration of 18 μM) and cells incubated for a further 10 min to label nascent polypeptide chains. Background labelling is determined by incubating cells without puromycin. Cells are then washed in HBSS, harvested by scraping and centrifuged (300 g, 5 min). Cells are resuspended in 0.5 mL 50 mM DTT containing phosphatase inhibitors and incubated at 95℃ for 10 min. Samples are then snap frozen in liquid nitrogen and stored at -20℃ until blotted. Samples (20–30 μg protein/sample) are blotted onto a PVDF membrane. The membrane is blocked and incubated with anti-phospho-Thr183/Tyr185-JNK antibody overnight at 4℃. Secondary antibodies are used to label the primary antibody and detected using an infrared scanner. The intensity of the fluorescence signal for anti-phospho-JNK antibody is background corrected and normalized for loading.
Cell Research Cell lines Ehrlich ascites carcinoma (EAC) cells
Concentrations 500 ng/mL
Incubation Time 48 h
Method For the assay, EAC cells are plated in 96-well plates at a density of 10,000 cells/well/200 µL of medium. The cells are treated with the different concentrations of anisomycin for 48 h. Adriamycin (500 ng/mL) is used as a positive control. 0.5 mg/mL of MTT is added to each well. 4 h later, the formazan product of MTT reduction is dissolved in DMSO, and absorbance is measured at 570 nm using a Model 680 microplate reader.
Experimental Result Images Methods Biomarkers Images PMID
Western blot p-Keratin 20 / Keratin 20 / p-Keratin 18 / Keratin 18 / p-Keratin 8 / Keratin 8 / p-MK2 / p-hHSPB1 / hHSPB1 PP2A / PP2C p-ERK / ERK / p-JNK / JNK / p-p38 / ATF3 20724476
Immunofluorescence p-Keratin 8 / p-Keratin 18 / p-Keratin 20 20724476
Growth inhibition assay Cell viability 22684030
In Vivo
In vivo Peritumoral administration of anisomycin (5 mg/kg) significantly suppresses Ehrlich ascites carcinoma (EAC) growth resulting in the survival of approximately 60% of the mice 90 days after EAC inoculation.[4]
Animal Research Animal Models Male BALB/c mice
Dosages 5 mg/kg
Administration peritumorally

Chemical Information & Solubility

Molecular Weight 265.3 Formula

C14H19NO4
CAS No. 22862-76-6 SDF Download Anisomycin SDF
Smiles CC(=O)OC1C(CNC1CC2=CC=C(C=C2)OC)O
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 53 mg/mL ( (199.77 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 16 mg/mL

Water : Insoluble


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