Home MAPK JNK inhibitor BI-78D3 For research use only.

BI-78D3

BI-78D3 is a competitive JNK inhibitor with IC50 of 280 nM that displays > 100 fold selectivity over p38α and no activity at mTOR and PI-3K.

BI-78D3 Chemical Structure

BI-78D3 Chemical Structure

CAS No. 883065-90-5

Purity & Quality Control

Batch: S820101 DMSO]100 mg/mL]false]Water]Insoluble]false]Ethanol]Insoluble]false Purity: 99.57%
99.57

BI-78D3 Related Products

Signaling Pathway

JNK Signaling Pathways

JNK Signaling Pathway

Biological Activity

Description BI-78D3 is a competitive JNK inhibitor with IC50 of 280 nM that displays > 100 fold selectivity over p38α and no activity at mTOR and PI-3K.
Targets
JNK [1]
(Cell-free assay)
280 nM
In vitro
In vitro BI-78D3 Inhibits JNK interacting protein 1 (JIP1)-JNK binding and prevents JNK substrate phosphorylation[2].
Cell Research Cell lines Osteosarcoma cells (SaOS-2, MG-63, U2OS and LM7), primary (non-malignant) human osteoblasts(Hum63, Hum65 and Hum71)
Concentrations 10 nM
Incubation Time 24 h
Method

Cells are subjected to a dose range of doxorubicin concentrations in the presence or absence of a non-toxic dose (10 nM) of the small molecule JIP1-inhibitor BI-78D3.
In Vivo
In vivo In animal studies, BI-78D3 not only blocks JNK dependent Con A-induced liver damage but also restores insulin sensitivity in mouse models of type 2 diabetes[1].

Chemical Information & Solubility

Molecular Weight 379.37 Formula

C13H9N5O5S2
CAS No. 883065-90-5 SDF Download BI-78D3 SDF
Smiles C1COC2=C(O1)C=CC(=C2)N3C(=O)NN=C3SC4=NC=C(S4)[N+](=O)[O-]
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 100 mg/mL ( (263.59 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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