BI-78D3

Catalog No.S8201 Batch:S820101

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Technical Data

Formula

C13H9N5O5S2
Molecular Weight 379.37 CAS No. 883065-90-5
Solubility (25°C)* In vitro DMSO 100 mg/mL (263.59 mM)
Water Insoluble
Ethanol Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description BI-78D3 is a competitive JNK inhibitor with IC50 of 280 nM that displays > 100 fold selectivity over p38α and no activity at mTOR and PI-3K.
Targets
JNK [1]
(Cell-free assay)
280 nM
In vitro BI-78D3 Inhibits JNK interacting protein 1 (JIP1)-JNK binding and prevents JNK substrate phosphorylation[2].
In vivo In animal studies, BI-78D3 not only blocks JNK dependent Con A-induced liver damage but also restores insulin sensitivity in mouse models of type 2 diabetes[1].

Protocol (from reference)

Cell Assay:

[2]
  • Cell lines

    Osteosarcoma cells (SaOS-2, MG-63, U2OS and LM7), primary (non-malignant) human osteoblasts(Hum63, Hum65 and Hum71)

  • Concentrations

    10 nM

  • Incubation Time

    24 h

  • Method

    Cells are subjected to a dose range of doxorubicin concentrations in the presence or absence of a non-toxic dose (10 nM) of the small molecule JIP1-inhibitor BI-78D3.

Selleck's BI-78D3 has been cited by 2 publications

p85β alters response to EGFR inhibitor in ovarian cancer through p38 MAPK-mediated regulation of DNA repair [ Neoplasia, 2021, 23(7):718-730] PubMed: 34144267
Targeting JNK pathway promotes human hematopoietic stem cell expansion. [ Cell Discov, 2019, 5:2] PubMed: 30622738

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.