Home Metabolism P450 (e.g. CYP17) inhibitor Alizarin For research use only.

Alizarin

Synonyms: Anthraquinonic

Alizarin is a red coloring mordant dye extracted from the roots of madder plants (Rubia tinctorum L). Alizarin strongly inhibits P450 isoform CYP1A1, CYP1A2, and CYP1B1 with IC50 of 6.2 μM, 10.0 μM, and 2.7 μM, respectively in an enzymatic assay. There are also literature reports Alizarin increases the CYP1A1 enzymatic activity and acts as an agonist to the AHR receptor in HepG2 cells.

Alizarin Chemical Structure

Alizarin Chemical Structure

CAS No. 72-48-0

Purity & Quality Control

Batch: S252601 DMSO]48 mg/mL]false]Water]Insoluble]false]Ethanol]Insoluble]false Purity: 99.19%
99.19

Alizarin Related Products

Signaling Pathway

P450 (e.g. CYP17) Signaling Pathways

P450 (e.g. CYP17) Signaling Pathway

Biological Activity

Description Alizarin is a red coloring mordant dye extracted from the roots of madder plants (Rubia tinctorum L). Alizarin strongly inhibits P450 isoform CYP1A1, CYP1A2, and CYP1B1 with IC50 of 6.2 μM, 10.0 μM, and 2.7 μM, respectively in an enzymatic assay. There are also literature reports Alizarin increases the CYP1A1 enzymatic activity and acts as an agonist to the AHR receptor in HepG2 cells.
Targets
CYP1B1 [1] CYP1A1 [1] CYP1A2 [1]
2.7 μM 6.2 μM 10.0 μM
In vitro
In vitro Alizarin weakly inhibits CYP2A6 and CYP2E1. Alizarin shows competitive inhibition against CYP1B1 with Ki of 0.5 μM. Alizarin reduces the mutagenicity of MeIQx, which induced by each CYP1A2 or CYP1B1, while does not effectively reduce the mutation induced by B[a]P. [1] Alizarin exhibits antioxidants against iodophenol-derived phenoxyl radicals, superoxide anion radicals and lipid peroxidation in rat liver microsomes. [2]
In Vivo
In vivo Alizarin also reduces the hepatic content of thiobarbituric acid-reactive substances and the serum level of alanine aminotransferase in poisoned animals. [2]

Chemical Information & Solubility

Molecular Weight 240.21 Formula

C14H8O4
CAS No. 72-48-0 SDF Download Alizarin SDF
Smiles C1=CC=C2C(=C1)C(=O)C3=C(C2=O)C(=C(C=C3)O)O
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 48 mg/mL ( (199.82 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

Mass Concentration Volume Molecular Weight

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy Alizarin | Alizarin supplier | purchase Alizarin | Alizarin cost | Alizarin manufacturer | order Alizarin | Alizarin distributor