Home PI3K/Akt/mTOR PI3K activator 740 Y-P (PDGFR 740Y-P) For research use only.

740 Y-P (PDGFR 740Y-P)

Synonyms: 740YPDGFR

740 Y-P (PDGFR 740Y-P, 740YPDGFR) is a cell-permeable phosphopeptide activator of PI3K.

740 Y-P (PDGFR 740Y-P) Chemical Structure

740 Y-P (PDGFR 740Y-P) Chemical Structure

CAS No. 1236188-16-1

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740 Y-P (PDGFR 740Y-P) Related Products

Signaling Pathway

PI3K Signaling Pathways

PI3K Signaling Pathway

Biological Activity

Description 740 Y-P (PDGFR 740Y-P, 740YPDGFR) is a cell-permeable phosphopeptide activator of PI3K.
Targets
PI3K [1]
In vitro
In vitro

The PDGFR 740Y-P peptide stimulates a mitogenic response in muscle cells. The ability of the 740Y-P peptide to stimulate mitogenesis is highly specific and not a general feature of a cell permeable SH2 domain binding peptides[2].

740Y-P is as effective as a growth factor (FGF2) at promoting neuronal cell survival via the established PI 3-kinase-Akt survival cascade. Stimulation of neuronal cell survival by the PDGFR740Y-P peptide is dose dependent and does not require insulin[1].
Cell Research Cell lines NIH 3T3 cells
Concentrations 50 μg/ml
Incubation Time 2 h
Method

NIH 3T3 cells (2×106) are incubated in suspension at 37°C in Ca2+ and Mn2+ free HBSS, 10% FCS containing either 50 μg/ml of 740Y-P peptide or an equal volume of PBS as a control. After 2h cells are centrifuged, washed and trypsinised to degrade non-internalised peptide. Cells are then resuspended in lysis buffer (50 mM Tris pH 7.4, 150 mM NaCl, 10% Glycerol, 2% NP40, 0.25% deoxycholate, 1 mM EDTA, 1 mM Vanadate, Protease inhibitors "complete" cocktail from Boehringer-Mannheim) for 1 h at 4°C. Lysates are clarified by centrifugation at 1.4×104g for 5 min and the supernatants are incubated for 1h with streptavidin-agarose beads. Beads are subsequently washed three times in lysis buffer and finally boiled in SDS sample buffer and resolved by SDS-PAGE on a 12% gel, transferred to nitrocellulose and immunoblotted with the p85 monoclonal antibody.
In Vivo
In vivo

740 Y-P decreases the degree of ROS levels in Aβ(25-32) treated hippocampal tissues and increases the extent of AKT and PI3K phosphorylation in Alzheimer’s disease rat model.

Chemical Information & Solubility

Molecular Weight 3270.70 Formula

C141H222N43O39PS3
CAS No. 1236188-16-1 SDF Download 740 Y-P (PDGFR 740Y-P) SDF
Smiles CCC(C)C(C(=O)NC(CCCCN)C(=O)NC(C(C)CC)C(=O)NC(CC1=CNC2=CC=CC=C21)C(=O)NC(CC3=CC=CC=C3)C(=O)NC(CCC(=O)N)C(=O)NC(CC(=O)N)C(=O)NC(CCCNC(=N)N)C(=O)NC(CCCNC(=N)N)C(=O)NC(CCSC)C(=O)NC(CCCCN)C(=O)NC(CC4=CNC5=CC=CC=C54)C(=O)NC(CCCCN)C(=O)NC(CCCCN)C(=O)NC(CO)C(=O)NC(CC(=O)O)C(=O)NCC(=O)NCC(=O)NC(CC6=CC=C(C=C6)OP(=O)(O)O)C(=O)NC(CCSC)C(=O)NC(CC(=O)O)C(=O)NC(CCSC)C(=O)NC(CO)C(=O)O)NC(=O)C(CCC(=O)N)NC(=O)C(CCCNC(=N)N)N
Storage (From the date of receipt) 3 years -20°C powder (seal)

In vitro
Batch:

DMSO : 100 mg/mL ( (30.57 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 8 mg/mL

Ethanol : Insoluble


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In vivo
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