Home Metabolism Carbohydrate Metabolism modulator 1-Deoxynojirimycin For research use only.

1-Deoxynojirimycin

Synonyms: duvoglustat, moranolin

1-Deoxynojirimycin (duvoglustat, moranolin) is a potent α-glucosidase inhibitor and most commonly found in mulberry leaves. It has therapeutic potency against diabetes mellitus.

1-Deoxynojirimycin Chemical Structure

1-Deoxynojirimycin Chemical Structure

CAS No. 19130-96-2

Purity & Quality Control

1-Deoxynojirimycin Related Products

Biological Activity

Description 1-Deoxynojirimycin (duvoglustat, moranolin) is a potent α-glucosidase inhibitor and most commonly found in mulberry leaves. It has therapeutic potency against diabetes mellitus.
Targets
α-glucosidase [3]
(Cell-free assay)
In vitro
In vitro 1-Deoxynojirimycin (DNJ) up to 200 μmol/L does not influence survival of HUVECs. DNJ behaves as an antioxidant, decreases ROS production, and delays cellular senescence[2].
Cell Research Cell lines HUVECs
Concentrations 0-200 μmol/L
Incubation Time 24 or 48 h
Method

Cytotoxicity of DNJ is assessed using a WST-8 assay. HUVECs at 80% confluency are trypsinized and transferred to 96-well plates (4000 cells/well). Stock solutions of DNJ are prepared in HuMedia-EG2 medium. DNJ test media are prepared from the stock solutions and diluted to final concentrations of 0-200 μmol/L in HuMedia-EG2 medium. After incubation for 24 h at 37℃, the cells are placed in 200 μL of fresh HuMedia-EG2 medium with various concentrations of DNJ. After 24 or 48 h, 10 μL WST-8 solution is added to each well. After incubation for 3 h at 37℃, cytotoxicity is measured using a microplate reader at a wavelength of 450 nm and a reference wavelength of 655 nm.
In Vivo
In vivo 1-Deoxynojirimycin (DNJ) inhibits intestinal glucose absorption in intestine. 1-Deoxynojirimycin down-regulates intestinal SGLT1, Na+/K+-ATP and GLUT2 mRNA and protein expression. Pretreatment with DNJ (50 mg/kg) increases the activity, mRNA and protein levels of hepatic glycolysis enzymes (GK, PFK, PK, PDE1) and decreases the expression of gluconeogenesis enzymes (PEPCK, G-6-Pase), improves glucose tolerance in both normal and diabetic mice. DNJ inhibits intestinal glucose absorption and accelerates hepatic glucose metabolism by directly regulating the expression of proteins involved in glucose transport systems, glycolysis and gluconeogenesis enzymes[1]. DNJ reduces oxidative stress in the liver and plasma in rodents[2].
Animal Research Animal Models ICR mice
Dosages 50 mg/kg
Administration administered intragastrically
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT01380743 Completed
Pompe Disease
Amicus Therapeutics
 October 31 2011 Phase 2

Chemical Information & Solubility

Molecular Weight 163.17 Formula

C6H13NO4
CAS No. 19130-96-2 SDF Download 1-Deoxynojirimycin SDF
Smiles C1C(C(C(C(N1)CO)O)O)O
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 33 mg/mL ( (202.24 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 33 mg/mL

Ethanol : Insoluble


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In vivo
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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