Home Metabolism Carbohydrate Metabolism inhibitor Bromopyruvic acid (3-BP) For research use only.

Bromopyruvic acid (3-BP)

Synonyms: 3-Bromopyruvic acid, 3-Bromopyruvate, Hexokinase II Inhibitor II

Bromopyruvic acid (3-BP) is a hexokinase II inhibitor with Ki of 2.4 mM for glycolysis/hexokinase inhibition. It is inhibitor of tumour cell energy metabolism and chemopotentiator of platinum drugs.

Bromopyruvic acid (3-BP) Chemical Structure

Bromopyruvic acid (3-BP) Chemical Structure

CAS No. 1113-59-3

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Bromopyruvic acid (3-BP) Related Products

Biological Activity

Description Bromopyruvic acid (3-BP) is a hexokinase II inhibitor with Ki of 2.4 mM for glycolysis/hexokinase inhibition. It is inhibitor of tumour cell energy metabolism and chemopotentiator of platinum drugs.
In vitro
In vitro

Bromopyruvic acid, a glycolysis inhibitor, maintains haploidy to a certain extent in both OdG and EG1 haESCs, with no effect on the proliferation or pluripotency of the haESCs.[2]
Cell Research Cell lines Mouse haESC lines AGH-OG-3 and AGH-EG-1
Concentrations 12.5 μg/mL
Incubation Time 12 days
Method

Mouse haESC lines AGH-OG-3 and AGH-EG-1 were cultured in M2iL mediums, which containing knockout DMEM, 15% fetal bovine serum, 1% GlutaMAX, 1% nonessential amino acids, 100 μM β-mercaptoethanol, 100 U Leukemia inhibitory factor, 3 μM CHIR99021, and 1 μM PD0325901. The cells are cultured at 37 ℃ with 5% CO2 in a humidified environment. 1,25-(OH)2D3 and 3-BrPA are used at a final concentration of 12.5 μg/mL and 5μM, respectively.
In Vivo
In vivo

Bromopyruvic acid reduces the growth of RKO cells with CD36 knockdown at a significantly slower rate in xenograft model.[3]
Animal Research Animal Models 4-week-old nude mice injected with RKO cells
Dosages 5 mg/kg/day
Administration i.p.

Chemical Information & Solubility

Molecular Weight 166.96 Formula

C3H3BrO3
CAS No. 1113-59-3 SDF --
Smiles C(C(=O)C(=O)O)Br
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 33 mg/mL ( (197.65 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 33 mg/mL

Ethanol : 33 mg/mL


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In vivo
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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