1-Deoxynojirimycin

Catalog No.S3839 Batch:S383901

Technical Data

Formula	C₆H₁₃NO₄
Molecular Weight	163.17	CAS No.	19130-96-2
Solubility (25°C)*	In vitro	DMSO	16 mg/mL (98.05 mM)


* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

1-Deoxynojirimycin (duvoglustat, moranolin) is a potent α-glucosidase inhibitor and most commonly found in mulberry leaves. It has therapeutic potency against diabetes mellitus.

Targets

α-glucosidase ^[3]
(Cell-free assay)

In vitro

1-Deoxynojirimycin (DNJ) up to 200 μmol/L does not influence survival of HUVECs. DNJ behaves as an antioxidant, decreases ROS production, and delays cellular senescence^[2].

In vivo

1-Deoxynojirimycin (DNJ) inhibits intestinal glucose absorption in intestine. 1-Deoxynojirimycin down-regulates intestinal SGLT1, Na+/K+-ATP and GLUT2 mRNA and protein expression. Pretreatment with DNJ (50 mg/kg) increases the activity, mRNA and protein levels of hepatic glycolysis enzymes (GK, PFK, PK, PDE1) and decreases the expression of gluconeogenesis enzymes (PEPCK, G-6-Pase), improves glucose tolerance in both normal and diabetic mice. DNJ inhibits intestinal glucose absorption and accelerates hepatic glucose metabolism by directly regulating the expression of proteins involved in glucose transport systems, glycolysis and gluconeogenesis enzymes^[1]. DNJ reduces oxidative stress in the liver and plasma in rodents^[2].

Protocol (from reference)

Cell Assay:^{^[2]}	Cell lines HUVECs Concentrations 0-200 μmol/L Incubation Time 24 or 48 h Method Cytotoxicity of DNJ is assessed using a WST-8 assay. HUVECs at 80% confluency are trypsinized and transferred to 96-well plates (4000 cells/well). Stock solutions of DNJ are prepared in HuMedia-EG2 medium. DNJ test media are prepared from the stock solutions and diluted to final concentrations of 0-200 μmol/L in HuMedia-EG2 medium. After incubation for 24 h at 37℃, the cells are placed in 200 μL of fresh HuMedia-EG2 medium with various concentrations of DNJ. After 24 or 48 h, 10 μL WST-8 solution is added to each well. After incubation for 3 h at 37℃, cytotoxicity is measured using a microplate reader at a wavelength of 450 nm and a reference wavelength of 655 nm.
Animal Study:^{^[1]}	Animal Models ICR mice Dosages 50 mg/kg Administration administered intragastrically

Cell Assay:^{^[2]}

Cell lines
HUVECs
Concentrations
0-200 μmol/L
Incubation Time
24 or 48 h
Method
Cytotoxicity of DNJ is assessed using a WST-8 assay. HUVECs at 80% confluency are trypsinized and transferred to 96-well plates (4000 cells/well). Stock solutions of DNJ are prepared in HuMedia-EG2 medium. DNJ test media are prepared from the stock solutions and diluted to final concentrations of 0-200 μmol/L in HuMedia-EG2 medium. After incubation for 24 h at 37℃, the cells are placed in 200 μL of fresh HuMedia-EG2 medium with various concentrations of DNJ. After 24 or 48 h, 10 μL WST-8 solution is added to each well. After incubation for 3 h at 37℃, cytotoxicity is measured using a microplate reader at a wavelength of 450 nm and a reference wavelength of 655 nm.

Animal Study:^{^[1]}

Animal Models
ICR mice
Dosages
50 mg/kg
Administration
administered intragastrically

References

Selleck's 1-Deoxynojirimycin has been cited by 1 publication

Transmembrane serine protease TMPRSS2 implicated in SARS-CoV-2 infection is autoactivated intracellularly and requires N-glycosylation for regulation [ J Biol Chem, 2022, 298(12):102643]	PubMed: 36309092

Transmembrane serine protease TMPRSS2 implicated in SARS-CoV-2 infection is autoactivated intracellularly and requires N-glycosylation for regulation [ J Biol Chem, 2022, 298(12):102643]

PubMed: 36309092

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SHIPPING AND STORAGE
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