1-Deoxynojirimycin

Catalog No.S3839 Batch:S383902

Technical Data

Formula

C₆H₁₃NO₄

Molecular Weight

163.17

CAS No.

19130-96-2

Solubility (25°C)*

In vitro

DMSO

33 mg/mL (202.24 mM)

Water

33 mg/mL (202.24 mM)

Ethanol

Insoluble

In vivo (Add solvents to the product individually and in order)

Clear solution	5% DMSO 1 95% Corn oil	0.27mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 5.4 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O	1.65mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 33 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

1-Deoxynojirimycin (duvoglustat, moranolin) is a potent α-glucosidase inhibitor and most commonly found in mulberry leaves. It has therapeutic potency against diabetes mellitus.

Targets

α-glucosidase ^[3]
(Cell-free assay)

In vitro

1-Deoxynojirimycin (DNJ) up to 200 μmol/L does not influence survival of HUVECs. DNJ behaves as an antioxidant, decreases ROS production, and delays cellular senescence^[2].

In vivo

1-Deoxynojirimycin (DNJ) inhibits intestinal glucose absorption in intestine. 1-Deoxynojirimycin down-regulates intestinal SGLT1, Na+/K+-ATP and GLUT2 mRNA and protein expression. Pretreatment with DNJ (50 mg/kg) increases the activity, mRNA and protein levels of hepatic glycolysis enzymes (GK, PFK, PK, PDE1) and decreases the expression of gluconeogenesis enzymes (PEPCK, G-6-Pase), improves glucose tolerance in both normal and diabetic mice. DNJ inhibits intestinal glucose absorption and accelerates hepatic glucose metabolism by directly regulating the expression of proteins involved in glucose transport systems, glycolysis and gluconeogenesis enzymes^[1]. DNJ reduces oxidative stress in the liver and plasma in rodents^[2].

Protocol (from reference)

Cell Assay:^{^[2]}	Cell lines HUVECs Concentrations 0-200 μmol/L Incubation Time 24 or 48 h Method Cytotoxicity of DNJ is assessed using a WST-8 assay. HUVECs at 80% confluency are trypsinized and transferred to 96-well plates (4000 cells/well). Stock solutions of DNJ are prepared in HuMedia-EG2 medium. DNJ test media are prepared from the stock solutions and diluted to final concentrations of 0-200 μmol/L in HuMedia-EG2 medium. After incubation for 24 h at 37℃, the cells are placed in 200 μL of fresh HuMedia-EG2 medium with various concentrations of DNJ. After 24 or 48 h, 10 μL WST-8 solution is added to each well. After incubation for 3 h at 37℃, cytotoxicity is measured using a microplate reader at a wavelength of 450 nm and a reference wavelength of 655 nm.
Animal Study:^{^[1]}	Animal Models ICR mice Dosages 50 mg/kg Administration administered intragastrically

Cell Assay:^{^[2]}

Cell lines
HUVECs
Concentrations
0-200 μmol/L
Incubation Time
24 or 48 h
Method
Cytotoxicity of DNJ is assessed using a WST-8 assay. HUVECs at 80% confluency are trypsinized and transferred to 96-well plates (4000 cells/well). Stock solutions of DNJ are prepared in HuMedia-EG2 medium. DNJ test media are prepared from the stock solutions and diluted to final concentrations of 0-200 μmol/L in HuMedia-EG2 medium. After incubation for 24 h at 37℃, the cells are placed in 200 μL of fresh HuMedia-EG2 medium with various concentrations of DNJ. After 24 or 48 h, 10 μL WST-8 solution is added to each well. After incubation for 3 h at 37℃, cytotoxicity is measured using a microplate reader at a wavelength of 450 nm and a reference wavelength of 655 nm.

Animal Study:^{^[1]}

Animal Models
ICR mice
Dosages
50 mg/kg
Administration
administered intragastrically

References

Selleck's 1-Deoxynojirimycin has been cited by 1 publication

Transmembrane serine protease TMPRSS2 implicated in SARS-CoV-2 infection is autoactivated intracellularly and requires N-glycosylation for regulation [ J Biol Chem, 2022, 298(12):102643]	PubMed: 36309092

Transmembrane serine protease TMPRSS2 implicated in SARS-CoV-2 infection is autoactivated intracellularly and requires N-glycosylation for regulation [ J Biol Chem, 2022, 298(12):102643]

PubMed: 36309092

RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.

SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.