Home Cytoskeletal Signaling Antineoplastic and Immunosuppressive Antibiotics inhibitor - Microtubule Associated inhibitor - AChR inhibitor - Apoptosis related activator - Autophagy activator Vinblastine sulfate For research use only.

Vinblastine sulfate

Synonyms: NSC-49842, Vincaleukoblastine, 29060-LE

Vinblastine sulfate inhibits microtubule formation and suppresses nAChR activity with IC50 of 8.9 μM in a cell-free assay, used to treat certain kinds of cancer. Vinblastine sulfate induces autophagy and apoptosis.

Vinblastine sulfate Chemical Structure

Vinblastine sulfate Chemical Structure

CAS No. 143-67-9

Purity & Quality Control

Vinblastine sulfate Related Products

Cell Data

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
COLO 320 human colorectal carcinoma cell line Function assay In vitro concentration required to kill 50% of COLO 320 human colorectal carcinoma cell line, EC50=0.08 μM 12361397
LNCaP human prostate cancer cell line Function assay In vitro concentration required to kill 50% of LNCaP human prostate cancer cell line, EC50=0.5 μM 12361397
K562 cell Growth inhibition assay In vitro inhibitory concentration against human chronic myelogenous leukemia K562 cell growth, IC50=0.001 μM 12852768
T47D cells Function assay In vitro concentration required to kill 50% of T47D human breast ductal carcinoma cell line, EC50=0.08 μM 12361397
K562 cell Proliferation assay 48 h Antiproliferative activity against human K562 cells after 48 hrs, IC50=0.001 μM 19220018
ACHN cells Cytotoxicity assay 48 h Cytotoxicity against human ACHN cells after 48 hrs by SRB assay, IC50=22.7 μM 19467877
A375 cells Cytotoxicity assay 48 h Cytotoxicity against human A375 cells after 48 hrs by SRB assay, IC50=7.2 μM 19467877
C32 cells Cytotoxicity assay 48 h Cytotoxicity against human C32 cells after 48 hrs by SRB assay, IC50=3 μM 19467877
LNCAP cells Cytotoxicity assay 48 h Cytotoxicity against human LNCAP cells after 48 hrs by SRB assay, IC50=29.3 μM 19467877
Huh-7D12 cells Cytotoxicity assay 48 h Cytotoxicity against human Huh-7D12 cells after 48 hrs by SRB assay, IC50=45.6 μM 19467877
COR-L23 cells Cytotoxicity assay 48 h Cytotoxicity against human COR-L23 cells after 48 hrs by SRB assay, IC50=45.5 μM 19467877
142BR cells Cytotoxicity assay 48 h Cytotoxicity against human 142BR cells after 48 hrs by SRB assay, IC50=37.6 μM 19467877
HT-29 cells Cytotoxicity assay 48 h Cytotoxicity against human HT-29 cells after 48 hrs by MTS assay, IC50=0.55 μM 21920762
A549 cells Proliferation assay 48 h Antiproliferative activity against human A549 cells after 48 hrs by MTS assay, IC50=2.36 μM 22546674
DU145 cells Proliferation assay 48 h Antiproliferative activity against human DU145 cells after 48 hrs by MTS assay, IC50=4.25 μM 22546674
SK-MEL-5 cells Proliferation assay 48 h Antiproliferative activity against human SK-MEL-5 cells after 48 hrs by MTS assay, IC50=1.74 μM 22546674
HepG2 cells Proliferation assay 48 h Antiproliferative activity against human HepG2 cells after 48 hrs by MTS assay, IC50=0.16 μM 22546674
HT-29 cells Proliferation assay 48 h Antiproliferative activity against human HT-29 cells after 48 hrs by MTS assay, IC50=11.18 μM 22546674
MCF7 cells Proliferation assay 48 h Antiproliferative activity against human MCF7 cells after 48 hrs by MTS assay, IC50=24.08 μM 22546674
MDA-MB-231 cells Proliferation assay 48 h Antiproliferative activity against human MDA-MB-231 cells after 48 hrs by MTS assay, IC50=31.52 μM 22546674
rat REF52 cells Function assay 0.1 μM 30 mins Induction of microtubule depolymerization in rat REF52 cells at 0.1 uM after 30 mins by fluorescence assay 23947826
HepG2 cells Cytotoxicity assay 72 h Cytotoxicity against adriamycin-resistant human HepG2 cells assessed as growth inhibition after 72 hrs by MTT assay, IC50=0.056 μM 23708010
HepG2 cells Cytotoxicity assay 72 h Cytotoxicity against human HepG2 cells assessed as growth inhibition after 72 hrs by MTT assay, IC50=0.019 μM 23708010
K562 cells Cytotoxicity assay 72 h Cytotoxicity against human K562 cells assessed as growth inhibition after 72 hrs by MTT assay, IC50=0.016 μM 23708010
MDA-MB-231 cells Cytotoxicity assay 72 h Cytotoxicity against human MDA-MB-231 cells assessed as growth inhibition after 72 hrs by MTT assay, IC50=0.0083 μM 23708010
MCF7 cells Cytotoxicity assay 72 h Cytotoxicity against human MCF7 cells assessed as growth inhibition after 72 hrs by MTT assay, IC50=0.007 μM 23708010
SCL6 cells Cytotoxicity assay Cytotoxicity against human SCL6 cells by MTT assay, ED50=6.1 Μm 12880314
SCL9 Cytotoxicity assay Cytotoxicity against human SCL9 cells by MTT assay, ED=5.3 μM 12880314
KATO III cells Cytotoxicity assay Cytotoxicity against human KATO III cells by MTT assay, ED50=6.1 μM 12880314
NUGC4 cells Cytotoxicity assay Cytotoxicity against human NUGC4 cells by MTT assay, ED50=5.3 μM 12880314
UACC903 cells Cytotoxicity assay 48 h Cytotoxicity against human UACC903 cells after 48 hrs by MTS assay, IC50=1.65 μM 21920762
K562 cells Function assay Inhibition of tubulin polymerization in human K562 cells, IC50=0.13 μM 20546980
BxPC3 cells Proliferation assay 48 h Antiproliferative activity against human BxPC3 cells after 48 hrs by MTS assay, IC50=1.13 μM 22546674
Click to View More Cell Line Experimental Data

Biological Activity

Description Vinblastine sulfate inhibits microtubule formation and suppresses nAChR activity with IC50 of 8.9 μM in a cell-free assay, used to treat certain kinds of cancer. Vinblastine sulfate induces autophagy and apoptosis.
Targets
nAChR [1]
(Adrenal Chromaffin Cells)
8.9 μM
In vitro
In vitro The average terminal half-lives of Vinblastine is 14.3 h. When incubated in freshly isolated rat hepatocytes, VLB penetrates rapidly and intensely into the cells, probably through a passive diffusion mechanism followed by tight cellular binding[3]. Vinblastine inhibits the angiogenic response induced by adrenomedullin and is also positive for mitotic slippage, causing micronuclei in mononucleate cells with cytokinesis block[4]. vinblastine gives significant increase in micronucleated mononucleated cells at concentrations that produced approximately 50% cell death and cytostasis or less as calculated using RPD, RICC and RCC[2].
Cell Research Cell lines Chinese hamster ovary (CHO) cells
Concentrations 1% (v/v) (dissolved in DMSO)
Incubation Time 3h
Method

Six-well treatment plates are set up that contained 5 × 104 cells/mL in each well, suspended in 3 mL culture medium, and these are treated with vinblastine for 3 h followed by 21 h growth.
Experimental Result Images Methods Biomarkers Images PMID
Western blot GRP78 p-eIF2 p-JNK / c-Caspase-7 / c-PARP ERK / p-ERK / Mcl-1 / Bad / Bid / Noxa 19674193
Immunofluorescence α-tubulin / Acetyl tubulin 30120268
Growth inhibition assay Cell viability 27114800
In Vivo
In vivo Vinblastine is a widely used anticancer drug with undesired side effects [6]. A combination of VBL and RAP at very low doses against human HCC gets a satisfactory antiangiogenic effect in vivo[4]. The clinically relevant dose of vinblastine inhibits palmitoylation of tubulin in vivo in CEM cells (effect on depalmitoylation of tubulin)[5].
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT06381570 Recruiting
Low-grade Glioma
Daniel Morgenstern|The Hospital for Sick Children
 March 21 2024 Early Phase 1

Chemical Information & Solubility

Molecular Weight 909.05 Formula

C46H58N4O9.H2SO4
CAS No. 143-67-9 SDF Download Vinblastine sulfate SDF
Smiles CCC1(CC2CC(C3=C(CCN(C2)C1)C4=CC=CC=C4N3)(C5=C(C=C6C(=C5)C78CCN9C7C(C=CC9)(C(C(C8N6C)(C(=O)OC)O)OC(=O)C)CC)OC)C(=O)OC)O.OS(=O)(=O)O
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 100 mg/mL ( (110.0 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 50 mg/mL

Ethanol : Insoluble


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In vivo
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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