Home PI3K/Akt/mTOR AMPK activator Trans-ferulic acid For research use only.

Trans-ferulic acid

Trans-ferulic acid is a potent activator of AMPKunder high glucose condition.

Trans-ferulic acid Chemical Structure

Trans-ferulic acid Chemical Structure

CAS No. 537-98-4

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Trans-ferulic acid Related Products

Signaling Pathway

AMPK Signaling Pathways

AMPK Signaling Pathway

Biological Activity

Description Trans-ferulic acid is a potent activator of AMPKunder high glucose condition.
Targets
AMPK [1]
In vitro
In vitro

Trans-ferulic acid can promote the activation of AMPK and the phosphorylation of acetyl-CoA carboxylase in hyperglycemia-induced human liver cells (HepG2) and rat skeletal muscle cells (L6).Trans-ferulic acid reduces the level of intracellular reactive oxygen species (ROS) and nitric oxide (NO) induced by hyperglycemia and subsequently increases the level of glutathione.[2]
Cell Research Cell lines Human liver cells (HepG2), rat skeletal muscle cells (L6),
Concentrations 10, 20, 30 μM
Incubation Time 24 h
Method

Fully-differentiated L6 myotubes and HepG2 cells are sub-cultured in six-well plates at a cell density of 2.5 x 105 cells/well and incubated in fresh DMEM containing 5 mM glucose for the control well and 25 mM glucose for the remaining wells for 24 hr. After incubation, the cells are treated separately in the serum-free media with 10, 20, and 30 μM concentrations of trans-ferulic acid and incubated further for 24 hr. Then, the cell extraction is used for western blot.
In Vivo
In vivo

TFA exhibits a potential protective effect againstf cisplatin induced testicular injury by inhibiting oxidative stress as well as TLR4/IRF3/INF-γ, P38-MAPK/NF-κB-p65/TNF-α, and JAK1/STAT-3/ERK1/2 inflammatory signaling pathways.[2]
Animal Research Animal Models Sprague–Dawley rats
Dosages 50 mg/kg
Administration p.o.

Chemical Information & Solubility

Molecular Weight 194.18 Formula

C10H10O4
CAS No. 537-98-4 SDF --
Smiles COC1=C(O)C=CC(=C1)\C=C\C(O)=O
Storage (From the date of receipt) 2 years -20°C liquid

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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