Home PI3K/Akt/mTOR AMPK activator - Fatty Acid Synthase inhibitor A-769662 For research use only.

A-769662

A-769662 is a potent, reversible AMPK activator with EC50 of 0.8 μM in cell-free assays, little effect on GPPase/FBPase activity.

A-769662 Chemical Structure

A-769662 Chemical Structure

CAS No. 844499-71-4

Purity & Quality Control

A-769662 Related Products

Signaling Pathway

AMPK Signaling Pathways

AMPK Signaling Pathway

Cell Data

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
mouse hepatocytes Function assay 1 mM DMSO inhibits fatty acid synthesis with IC50 of 3.6 μM 16753576
rat hepatocytes Function assay 1 mM DMSO inhibits fatty acid synthesis with IC50 of 3.6 μM 16753576
HEK293 Kinase assay 200 μM DMSO activates endogenous AMPK 17728241
CCL13 Kinase assay 200 μM DMSO activates endogenous AMPK 17728241
MEFs Function assay 300 μM DMSO inhibits proteasomal function by an AMPK-independent mechanism 18593584
epididymal clear cells Function assay 200 μM DMSO inhibits the pH-mediated V-ATPase accumulation at the apical membrane 19211918
3T3-L1 Function assay 1.2 mM DMSO inhibits 3T3-L1 Adipogenesis 19483304
3T3-L1 Function assay 1.2 mM DMSO inhibits the Expression of AdipogenesisRelated Transcription Factors and Markers 19483304
3T3-L1 Function assay 1.2 mM DMSO inhibits Mitotic Clonal Expansion 19483304
3T3-L1 cytotoxicity assay 1.2 mM DMSO decreases Cell Viability 19483304
3T3-L1 Kinase assay 1.2 mM DMSO activates AMPK 19483304
L6 skeletal muscle cells Function assay 250 μM DMSO activates AMPK signaling pathways 19828836
L6 skeletal muscle cells Function assay 250 μM DMSO inhibits the Na+-K+-ATPase transport activity and cell surface abundance 19828836
MDA-MB231 Apoptosis assay 400 μM DMSO sensitizes human breast cancer cell lines to TRAIL-induced apoptosis 19896469
BT474 Apoptosis assay 400 μM DMSO sensitizes human breast cancer cell lines to TRAIL-induced apoptosis 19896469
MCF7 Apoptosis assay 400 μM DMSO sensitizes human breast cancer cell lines to TRAIL-induced apoptosis 19896469
Mesenchymal stem cells Kinase assay 10 µM DMSO induces a robust and sustained AMPK activation 24104879
Mesenchymal stem cells cytotoxicity assay 100 µM DMSO decreases the MSC proliferation 24104879
MG-63 cytotoxicity assay 10 µM DMSO inhibits H2O2-Induced Osteoblast Cell Death 24960362
MC3T3-E1 cytotoxicity assay 10 µM DMSO inhibits H2O2-Induced Osteoblast Cell Death 24960362
MG-63 Apoptosis assay 10 µM DMSO suppresses H2O2-Induced Osteoblast Cell Apoptosis 24960362
MC3T3-E1 Apoptosis assay 10 µM DMSO suppresses H2O2-Induced Osteoblast Cell Apoptosis 24960362
MG-63 Function assay 10 µM DMSO alleviates ROS accumulation and ATP depletion caused by H2O2 24960362
MC3T3-E1 Function assay 10 µM DMSO alleviates ROS accumulation and ATP depletion caused by H2O2 24960362
MG-63 Function assay 10 µM DMSO facilitates H2O2-induced autophagy activation 24960362
MC3T3-E1 Function assay 10 µM DMSO facilitates H2O2-induced autophagy activation 24960362
PC3 Kinase assay 100 µM DMSO upregulates the levels of AMPK and ACC phosphorylation 25594043
PC3M Kinase assay 100 µM DMSO upregulates the levels of AMPK and ACC phosphorylation 25594043
PC3 Function assay 100 µM DMSO induces PI3K/mTOR pathways 25594043
PC3M Function assay 100 µM DMSO induces PI3K/mTOR pathways 25594043
PC3 Growth inhibitory assay 100 µM DMSO suppresses proliferation 25594043
PC3M Growth inhibitory assay 100 µM DMSO suppresses proliferation 25594043
MC3T3-E1 Kinase assay 10 μM DMSO induces significant AMPK activation 26891866
MC3T3-E1 Growth inhibitory assay 10 μM DMSO inhibits Dex-induced osteoblast cell death 26891866
MC3T3-E1 Function assay 10 μM DMSO inhibits Dex-induced oxidative stress 26891866
BL21 Function assay 1 to 10 mins Binding affinity to human recombinant N-terminal AVI-tagged phosphorylated AMPK alpha1beta1gamma1 expressed in Escherichia coli BL21 cells after 1 to 10 mins by biolayer interferometry, Kd = 0.51 μM. 26510622
BL21 Function assay 1 to 10 mins Binding affinity to human recombinant N-terminal AVI-tagged phosphorylated AMPK alpha1beta2gamma1 expressed in Escherichia coli BL21 cells after 1 to 10 mins by biolayer interferometry, Kd = 14.5 μM. 26510622
HeLa Function assay 100 uM 1 hr Effect on AMPK activation in HeLa cells at 100 uM after 1 hr by immunoprecipitate kinase assay 17855357
HeLa Function assay 100 uM 1 hr Effect on AMPK phosphorylation in HeLa cells at 100 uM after 1 hr by immunoprecipitate kinase assay 17855357
HeLa Function assay 100 uM 5 mins Effect on AMPK phosphorylation in HeLa cells at 100 uM after 5 mins by immunoprecipitate kinase assay 17855357
HeLa Function assay 100 uM 1 hr Effect on acetyl-CoA carboxylase phosphorylation in HeLa cells at 100 uM after 1 hr by densitometry 17855357
HeLa Function assay 100 uM 1 hr Reduction of AMPK phosphorylation in HeLa cells at 100 uM after 1 hr in presence of STO-609 by immunoprecipitate kinase assay 17855357
HeLa Function assay 100 uM 1 hr Reduction of AMPK activation in HeLa cells at 100 uM after 1 hr in presence of STO-609 by immunoprecipitate kinase assay 17855357
HeLa Function assay 100 uM 1 hr Reduction of acetyl-CoA carboxylase phosphorylation in HeLa cells at 100 uM after 1 hr in presence of STO-609 by densitometry 17855357
CMK Growth Inhibition Assay Inhibition of human CMK cell growth in a cell viability assay, IC50 = 0.002202 μM. SANGER
SIG-M5 Growth Inhibition Assay Inhibition of human SIG-M5 cell growth in a cell viability assay, IC50 = 0.007856 μM. SANGER
MV-4-11 Growth Inhibition Assay Inhibition of human MV-4-11 cell growth in a cell viability assay, IC50 = 0.02274 μM. SANGER
GDM-1 Growth Inhibition Assay Inhibition of human GDM-1 cell growth in a cell viability assay, IC50 = 0.02553 μM. SANGER
A431 Growth Inhibition Assay Inhibition of human A431 cell growth in a cell viability assay, IC50 = 0.02919 μM. SANGER
NALM-6 Growth Inhibition Assay Inhibition of human NALM-6 cell growth in a cell viability assay, IC50 = 0.03166 μM. SANGER
KM12 Growth Inhibition Assay Inhibition of human KM12 cell growth in a cell viability assay, IC50 = 0.03213 μM. SANGER
QIMR-WIL Growth Inhibition Assay Inhibition of human QIMR-WIL cell growth in a cell viability assay, IC50 = 0.03642 μM. SANGER
NH-12 Growth Inhibition Assay Inhibition of human NH-12 cell growth in a cell viability assay, IC50 = 0.03689 μM. SANGER
KASUMI-1 Growth Inhibition Assay Inhibition of human KASUMI-1 cell growth in a cell viability assay, IC50 = 0.0418 μM. SANGER
ML-2 Growth Inhibition Assay Inhibition of human ML-2 cell growth in a cell viability assay, IC50 = 0.04344 μM. SANGER
YT Growth Inhibition Assay Inhibition of human YT cell growth in a cell viability assay, IC50 = 0.0467 μM. SANGER
Daudi Growth Inhibition Assay Inhibition of human Daudi cell growth in a cell viability assay, IC50 = 0.04979 μM. SANGER
OCI-AML2 Growth Inhibition Assay Inhibition of human OCI-AML2 cell growth in a cell viability assay, IC50 = 0.0572 μM. SANGER
ALL-PO Growth Inhibition Assay Inhibition of human ALL-PO cell growth in a cell viability assay, IC50 = 0.05793 μM. SANGER
MOLT-16 Growth Inhibition Assay Inhibition of human MOLT-16 cell growth in a cell viability assay, IC50 = 0.06191 μM. SANGER
EHEB Growth Inhibition Assay Inhibition of human EHEB cell growth in a cell viability assay, IC50 = 0.06325 μM. SANGER
AM-38 Growth Inhibition Assay Inhibition of human AM-38 cell growth in a cell viability assay, IC50 = 0.0644 μM. SANGER
C8166 Growth Inhibition Assay Inhibition of human C8166 cell growth in a cell viability assay, IC50 = 0.06619 μM. SANGER
RPMI-6666 Growth Inhibition Assay Inhibition of human RPMI-6666 cell growth in a cell viability assay, IC50 = 0.06798 μM. SANGER
BC-1 Growth Inhibition Assay Inhibition of human BC-1 cell growth in a cell viability assay, IC50 = 0.06973 μM. SANGER
CTV-1 Growth Inhibition Assay Inhibition of human CTV-1 cell growth in a cell viability assay, IC50 = 0.07303 μM. SANGER
HC-1 Growth Inhibition Assay Inhibition of human HC-1 cell growth in a cell viability assay, IC50 = 0.08314 μM. SANGER
JiyoyeP-2003 Growth Inhibition Assay Inhibition of human JiyoyeP-2003 cell growth in a cell viability assay, IC50 = 0.08371 μM. SANGER
RS4-11 Growth Inhibition Assay Inhibition of human RS4-11 cell growth in a cell viability assay, IC50 = 0.08572 μM. SANGER
PF-382 Growth Inhibition Assay Inhibition of human PF-382 cell growth in a cell viability assay, IC50 = 0.0859 μM. SANGER
G-361 Growth Inhibition Assay Inhibition of human G-361 cell growth in a cell viability assay, IC50 = 0.08815 μM. SANGER
LOXIMVI Growth Inhibition Assay Inhibition of human LOXIMVI cell growth in a cell viability assay, IC50 = 0.08833 μM. SANGER
A3-KAW Growth Inhibition Assay Inhibition of human A3-KAW cell growth in a cell viability assay, IC50 = 0.09169 μM. SANGER
EoL-1-cell Growth Inhibition Assay Inhibition of human EoL-1-cell cell growth in a cell viability assay, IC50 = 0.09558 μM. SANGER
LOUCY Growth Inhibition Assay Inhibition of human LOUCY cell growth in a cell viability assay, IC50 = 0.09641 μM. SANGER
HL-60 Growth Inhibition Assay Inhibition of human HL-60 cell growth in a cell viability assay, IC50 = 0.09739 μM. SANGER
MC116 Growth Inhibition Assay Inhibition of human MC116 cell growth in a cell viability assay, IC50 = 0.10014 μM. SANGER
CESS Growth Inhibition Assay Inhibition of human CESS cell growth in a cell viability assay, IC50 = 0.1011 μM. SANGER
REH Growth Inhibition Assay Inhibition of human REH cell growth in a cell viability assay, IC50 = 0.10382 μM. SANGER
HCC2157 Growth Inhibition Assay Inhibition of human HCC2157 cell growth in a cell viability assay, IC50 = 0.10455 μM. SANGER
HEL Growth Inhibition Assay Inhibition of human HEL cell growth in a cell viability assay, IC50 = 0.10728 μM. SANGER
BL-70 Growth Inhibition Assay Inhibition of human BL-70 cell growth in a cell viability assay, IC50 = 0.10805 μM. SANGER
NCI-H1770 Growth Inhibition Assay Inhibition of human NCI-H1770 cell growth in a cell viability assay, IC50 = 0.10865 μM. SANGER
SK-MM-2 Growth Inhibition Assay Inhibition of human SK-MM-2 cell growth in a cell viability assay, IC50 = 0.10944 μM. SANGER
MONO-MAC-6 Growth Inhibition Assay Inhibition of human MONO-MAC-6 cell growth in a cell viability assay, IC50 = 0.11372 μM. SANGER
SR Growth Inhibition Assay Inhibition of human SR cell growth in a cell viability assay, IC50 = 0.11387 μM. SANGER
MOLT-4 Growth Inhibition Assay Inhibition of human MOLT-4 cell growth in a cell viability assay, IC50 = 0.11446 μM. SANGER
KE-37 Growth Inhibition Assay Inhibition of human KE-37 cell growth in a cell viability assay, IC50 = 0.12924 μM. SANGER
ST486 Growth Inhibition Assay Inhibition of human ST486 cell growth in a cell viability assay, IC50 = 0.13527 μM. SANGER
HDLM-2 Growth Inhibition Assay Inhibition of human HDLM-2 cell growth in a cell viability assay, IC50 = 0.13616 μM. SANGER
ARH-77 Growth Inhibition Assay Inhibition of human ARH-77 cell growth in a cell viability assay, IC50 = 0.1406 μM. SANGER
EB2 Growth Inhibition Assay Inhibition of human EB2 cell growth in a cell viability assay, IC50 = 0.14234 μM. SANGER
CCRF-CEM Growth Inhibition Assay Inhibition of human CCRF-CEM cell growth in a cell viability assay, IC50 = 0.14576 μM. SANGER
HD-MY-Z Growth Inhibition Assay Inhibition of human HD-MY-Z cell growth in a cell viability assay, IC50 = 0.14791 μM. SANGER
BT-474 Growth Inhibition Assay Inhibition of human BT-474 cell growth in a cell viability assay, IC50 = 0.15074 μM. SANGER
KARPAS-45 Growth Inhibition Assay Inhibition of human KARPAS-45 cell growth in a cell viability assay, IC50 = 0.16984 μM. SANGER
Ramos-2G6-4C10 Growth Inhibition Assay Inhibition of human Ramos-2G6-4C10 cell growth in a cell viability assay, IC50 = 0.1715 μM. SANGER
MC-CAR Growth Inhibition Assay Inhibition of human MC-CAR cell growth in a cell viability assay, IC50 = 0.17153 μM. SANGER
MRK-nu-1 Growth Inhibition Assay Inhibition of human MRK-nu-1 cell growth in a cell viability assay, IC50 = 0.17751 μM. SANGER
MHH-PREB-1 Growth Inhibition Assay Inhibition of human MHH-PREB-1 cell growth in a cell viability assay, IC50 = 0.18242 μM. SANGER
SK-NEP-1 Growth Inhibition Assay Inhibition of human SK-NEP-1 cell growth in a cell viability assay, IC50 = 0.18514 μM. SANGER
BL-41 Growth Inhibition Assay Inhibition of human BL-41 cell growth in a cell viability assay, IC50 = 0.18943 μM. SANGER
CAS-1 Growth Inhibition Assay Inhibition of human CAS-1 cell growth in a cell viability assay, IC50 = 0.19307 μM. SANGER
NCI-H1522 Growth Inhibition Assay Inhibition of human NCI-H1522 cell growth in a cell viability assay, IC50 = 0.19355 μM. SANGER
CA46 Growth Inhibition Assay Inhibition of human CA46 cell growth in a cell viability assay, IC50 = 0.19556 μM. SANGER
L-363 Growth Inhibition Assay Inhibition of human L-363 cell growth in a cell viability assay, IC50 = 0.21121 μM. SANGER
NCI-H2087 Growth Inhibition Assay Inhibition of human NCI-H2087 cell growth in a cell viability assay, IC50 = 0.21446 μM. SANGER
GR-ST Growth Inhibition Assay Inhibition of human GR-ST cell growth in a cell viability assay, IC50 = 0.21764 μM. SANGER
OPM-2 Growth Inhibition Assay Inhibition of human OPM-2 cell growth in a cell viability assay, IC50 = 0.21791 μM. SANGER
U-698-M Growth Inhibition Assay Inhibition of human U-698-M cell growth in a cell viability assay, IC50 = 0.21915 μM. SANGER
CAL-148 Growth Inhibition Assay Inhibition of human CAL-148 cell growth in a cell viability assay, IC50 = 0.22342 μM. SANGER
L-428 Growth Inhibition Assay Inhibition of human L-428 cell growth in a cell viability assay, IC50 = 0.22499 μM. SANGER
DEL Growth Inhibition Assay Inhibition of human DEL cell growth in a cell viability assay, IC50 = 0.22825 μM. SANGER
LU-134-A Growth Inhibition Assay Inhibition of human LU-134-A cell growth in a cell viability assay, IC50 = 0.23116 μM. SANGER
MLMA Growth Inhibition Assay Inhibition of human MLMA cell growth in a cell viability assay, IC50 = 0.23399 μM. SANGER
DOHH-2 Growth Inhibition Assay Inhibition of human DOHH-2 cell growth in a cell viability assay, IC50 = 0.24513 μM. SANGER
RPMI-8226 Growth Inhibition Assay Inhibition of human RPMI-8226 cell growth in a cell viability assay, IC50 = 0.24596 μM. SANGER
NCI-H2196 Growth Inhibition Assay Inhibition of human NCI-H2196 cell growth in a cell viability assay, IC50 = 0.24776 μM. SANGER
COR-L279 Growth Inhibition Assay Inhibition of human COR-L279 cell growth in a cell viability assay, IC50 = 0.25293 μM. SANGER
CAPAN-1 Growth Inhibition Assay Inhibition of human CAPAN-1 cell growth in a cell viability assay, IC50 = 0.25589 μM. SANGER
NOMO-1 Growth Inhibition Assay Inhibition of human NOMO-1 cell growth in a cell viability assay, IC50 = 0.26322 μM. SANGER
SUP-T1 Growth Inhibition Assay Inhibition of human SUP-T1 cell growth in a cell viability assay, IC50 = 0.26516 μM. SANGER
MEG-01 Growth Inhibition Assay Inhibition of human MEG-01 cell growth in a cell viability assay, IC50 = 0.2687 μM. SANGER
CHP-126 Growth Inhibition Assay Inhibition of human CHP-126 cell growth in a cell viability assay, IC50 = 0.26909 μM. SANGER
HCC1599 Growth Inhibition Assay Inhibition of human HCC1599 cell growth in a cell viability assay, IC50 = 0.27841 μM. SANGER
EB-3 Growth Inhibition Assay Inhibition of human EB-3 cell growth in a cell viability assay, IC50 = 0.29293 μM. SANGER
WSU-NHL Growth Inhibition Assay Inhibition of human WSU-NHL cell growth in a cell viability assay, IC50 = 0.29672 μM. SANGER
COLO-684 Growth Inhibition Assay Inhibition of human COLO-684 cell growth in a cell viability assay, IC50 = 0.3025 μM. SANGER
LAMA-84 Growth Inhibition Assay Inhibition of human LAMA-84 cell growth in a cell viability assay, IC50 = 0.30816 μM. SANGER
TGW Growth Inhibition Assay Inhibition of human TGW cell growth in a cell viability assay, IC50 = 0.31095 μM. SANGER
NCI-SNU-1 Growth Inhibition Assay Inhibition of human NCI-SNU-1 cell growth in a cell viability assay, IC50 = 0.31106 μM. SANGER
MHH-NB-11 Growth Inhibition Assay Inhibition of human MHH-NB-11 cell growth in a cell viability assay, IC50 = 0.32291 μM. SANGER
COLO-824 Growth Inhibition Assay Inhibition of human COLO-824 cell growth in a cell viability assay, IC50 = 0.32838 μM. SANGER
U-87-MG Growth Inhibition Assay Inhibition of human U-87-MG cell growth in a cell viability assay, IC50 = 0.33314 μM. SANGER
HT Growth Inhibition Assay Inhibition of human HT cell growth in a cell viability assay, IC50 = 0.33482 μM. SANGER
THP-1 Growth Inhibition Assay Inhibition of human THP-1 cell growth in a cell viability assay, IC50 = 0.34164 μM. SANGER
HCC2218 Growth Inhibition Assay Inhibition of human HCC2218 cell growth in a cell viability assay, IC50 = 0.3418 μM. SANGER
KARPAS-422 Growth Inhibition Assay Inhibition of human KARPAS-422 cell growth in a cell viability assay, IC50 = 0.34433 μM. SANGER
EM-2 Growth Inhibition Assay Inhibition of human EM-2 cell growth in a cell viability assay, IC50 = 0.34443 μM. SANGER
TUR Growth Inhibition Assay Inhibition of human TUR cell growth in a cell viability assay, IC50 = 0.34453 μM. SANGER
MN-60 Growth Inhibition Assay Inhibition of human MN-60 cell growth in a cell viability assay, IC50 = 0.34596 μM. SANGER
DMS-153 Growth Inhibition Assay Inhibition of human DMS-153 cell growth in a cell viability assay, IC50 = 0.37276 μM. SANGER
J-RT3-T3-5 Growth Inhibition Assay Inhibition of human J-RT3-T3-5 cell growth in a cell viability assay, IC50 = 0.37831 μM. SANGER
EVSA-T Growth Inhibition Assay Inhibition of human EVSA-T cell growth in a cell viability assay, IC50 = 0.38095 μM. SANGER
SHP-77 Growth Inhibition Assay Inhibition of human SHP-77 cell growth in a cell viability assay, IC50 = 0.38298 μM. SANGER
D-283MED Growth Inhibition Assay Inhibition of human D-283MED cell growth in a cell viability assay, IC50 = 0.38569 μM. SANGER
GOTO Growth Inhibition Assay Inhibition of human GOTO cell growth in a cell viability assay, IC50 = 0.39536 μM. SANGER
KMOE-2 Growth Inhibition Assay Inhibition of human KMOE-2 cell growth in a cell viability assay, IC50 = 0.39664 μM. SANGER
U-266 Growth Inhibition Assay Inhibition of human U-266 cell growth in a cell viability assay, IC50 = 0.40452 μM. SANGER
NCI-H226 Growth Inhibition Assay Inhibition of human NCI-H226 cell growth in a cell viability assay, IC50 = 0.40703 μM. SANGER
KG-1 Growth Inhibition Assay Inhibition of human KG-1 cell growth in a cell viability assay, IC50 = 0.40876 μM. SANGER
NCI-H1882 Growth Inhibition Assay Inhibition of human NCI-H1882 cell growth in a cell viability assay, IC50 = 0.40912 μM. SANGER
KMS-12-PE Growth Inhibition Assay Inhibition of human KMS-12-PE cell growth in a cell viability assay, IC50 = 0.41877 μM. SANGER
KM-H2 Growth Inhibition Assay Inhibition of human KM-H2 cell growth in a cell viability assay, IC50 = 0.42374 μM. SANGER
NCI-H1417 Growth Inhibition Assay Inhibition of human NCI-H1417 cell growth in a cell viability assay, IC50 = 0.43916 μM. SANGER
RPMI-8402 Growth Inhibition Assay Inhibition of human RPMI-8402 cell growth in a cell viability assay, IC50 = 0.44161 μM. SANGER
EW-18 Growth Inhibition Assay Inhibition of human EW-18 cell growth in a cell viability assay, IC50 = 0.45104 μM. SANGER
HH Growth Inhibition Assay Inhibition of human HH cell growth in a cell viability assay, IC50 = 0.45751 μM. SANGER
NCI-H526 Growth Inhibition Assay Inhibition of human NCI-H526 cell growth in a cell viability assay, IC50 = 0.46847 μM. SANGER
NEC8 Growth Inhibition Assay Inhibition of human NEC8 cell growth in a cell viability assay, IC50 = 0.48029 μM. SANGER
P12-ICHIKAWA Growth Inhibition Assay Inhibition of human P12-ICHIKAWA cell growth in a cell viability assay, IC50 = 0.48862 μM. SANGER
DB Growth Inhibition Assay Inhibition of human DB cell growth in a cell viability assay, IC50 = 0.48893 μM. SANGER
SJRH30 Growth Inhibition Assay Inhibition of human SJRH30 cell growth in a cell viability assay, IC50 = 0.49478 μM. SANGER
ATN-1 Growth Inhibition Assay Inhibition of human ATN-1 cell growth in a cell viability assay, IC50 = 0.49906 μM. SANGER
ECC4 Growth Inhibition Assay Inhibition of human ECC4 cell growth in a cell viability assay, IC50 = 0.50901 μM. SANGER
LU-65 Growth Inhibition Assay Inhibition of human LU-65 cell growth in a cell viability assay, IC50 = 0.52007 μM. SANGER
NCI-H1092 Growth Inhibition Assay Inhibition of human NCI-H1092 cell growth in a cell viability assay, IC50 = 0.54078 μM. SANGER
NCI-H187 Growth Inhibition Assay Inhibition of human NCI-H187 cell growth in a cell viability assay, IC50 = 0.54464 μM. SANGER
LNCaP-Clone-FGC Growth Inhibition Assay Inhibition of human LNCaP-Clone-FGC cell growth in a cell viability assay, IC50 = 0.5472 μM. SANGER
LC4-1 Growth Inhibition Assay Inhibition of human LC4-1 cell growth in a cell viability assay, IC50 = 0.56308 μM. SANGER
NCI-H1436 Growth Inhibition Assay Inhibition of human NCI-H1436 cell growth in a cell viability assay, IC50 = 0.59043 μM. SANGER
NCI-H2171 Growth Inhibition Assay Inhibition of human NCI-H2171 cell growth in a cell viability assay, IC50 = 0.62179 μM. SANGER
LB647-SCLC Growth Inhibition Assay Inhibition of human LB647-SCLC cell growth in a cell viability assay, IC50 = 0.62241 μM. SANGER
NCI-H2141 Growth Inhibition Assay Inhibition of human NCI-H2141 cell growth in a cell viability assay, IC50 = 0.63266 μM. SANGER
NCI-H1304 Growth Inhibition Assay Inhibition of human NCI-H1304 cell growth in a cell viability assay, IC50 = 0.63388 μM. SANGER
Raji Growth Inhibition Assay Inhibition of human Raji cell growth in a cell viability assay, IC50 = 0.66976 μM. SANGER
NB69 Growth Inhibition Assay Inhibition of human NB69 cell growth in a cell viability assay, IC50 = 0.68575 μM. SANGER
NCI-H510A Growth Inhibition Assay Inhibition of human NCI-H510A cell growth in a cell viability assay, IC50 = 0.69172 μM. SANGER
COR-L88 Growth Inhibition Assay Inhibition of human COR-L88 cell growth in a cell viability assay, IC50 = 0.72456 μM. SANGER
K052 Growth Inhibition Assay Inhibition of human K052 cell growth in a cell viability assay, IC50 = 0.79127 μM. SANGER
SK-N-DZ Growth Inhibition Assay Inhibition of human SK-N-DZ cell growth in a cell viability assay, IC50 = 0.80231 μM. SANGER
KY821 Growth Inhibition Assay Inhibition of human KY821 cell growth in a cell viability assay, IC50 = 0.81743 μM. SANGER
MDA-MB-157 Growth Inhibition Assay Inhibition of human MDA-MB-157 cell growth in a cell viability assay, IC50 = 0.82406 μM. SANGER
MS-1 Growth Inhibition Assay Inhibition of human MS-1 cell growth in a cell viability assay, IC50 = 0.82745 μM. SANGER
L-540 Growth Inhibition Assay Inhibition of human L-540 cell growth in a cell viability assay, IC50 = 0.84843 μM. SANGER
MDA-MB-361 Growth Inhibition Assay Inhibition of human MDA-MB-361 cell growth in a cell viability assay, IC50 = 0.86048 μM. SANGER
NCI-SNU-5 Growth Inhibition Assay Inhibition of human NCI-SNU-5 cell growth in a cell viability assay, IC50 = 0.94375 μM. SANGER
KARPAS-299 Growth Inhibition Assay Inhibition of human KARPAS-299 cell growth in a cell viability assay, IC50 = 0.94828 μM. SANGER
NCI-H1694 Growth Inhibition Assay Inhibition of human NCI-H1694 cell growth in a cell viability assay, IC50 = 0.9773 μM. SANGER
P30-OHK Growth Inhibition Assay Inhibition of human P30-OHK cell growth in a cell viability assay, IC50 = 0.99292 μM. SANGER
LU-165 Growth Inhibition Assay Inhibition of human LU-165 cell growth in a cell viability assay, IC50 = 1.05606 μM. SANGER
NB1 Growth Inhibition Assay Inhibition of human NB1 cell growth in a cell viability assay, IC50 = 1.11241 μM. SANGER
UACC-812 Growth Inhibition Assay Inhibition of human UACC-812 cell growth in a cell viability assay, IC50 = 1.1488 μM. SANGER
RL Growth Inhibition Assay Inhibition of human RL cell growth in a cell viability assay, IC50 = 1.16282 μM. SANGER
NCI-H69 Growth Inhibition Assay Inhibition of human NCI-H69 cell growth in a cell viability assay, IC50 = 1.19391 μM. SANGER
SW1417 Growth Inhibition Assay Inhibition of human SW1417 cell growth in a cell viability assay, IC50 = 1.23671 μM. SANGER
NCI-H1155 Growth Inhibition Assay Inhibition of human NCI-H1155 cell growth in a cell viability assay, IC50 = 1.27588 μM. SANGER
NCI-H1581 Growth Inhibition Assay Inhibition of human NCI-H1581 cell growth in a cell viability assay, IC50 = 1.44028 μM. SANGER
MHH-CALL-2 Growth Inhibition Assay Inhibition of human MHH-CALL-2 cell growth in a cell viability assay, IC50 = 1.48514 μM. SANGER
SCLC-21H Growth Inhibition Assay Inhibition of human SCLC-21H cell growth in a cell viability assay, IC50 = 1.67837 μM. SANGER
LK-2 Growth Inhibition Assay Inhibition of human LK-2 cell growth in a cell viability assay, IC50 = 1.71089 μM. SANGER
CPC-N Growth Inhibition Assay Inhibition of human CPC-N cell growth in a cell viability assay, IC50 = 1.76764 μM. SANGER
SCC-3 Growth Inhibition Assay Inhibition of human SCC-3 cell growth in a cell viability assay, IC50 = 2.04311 μM. SANGER
NCI-H446 Growth Inhibition Assay Inhibition of human NCI-H446 cell growth in a cell viability assay, IC50 = 2.05293 μM. SANGER
RH-1 Growth Inhibition Assay Inhibition of human RH-1 cell growth in a cell viability assay, IC50 = 2.13933 μM. SANGER
NCI-H345 Growth Inhibition Assay Inhibition of human NCI-H345 cell growth in a cell viability assay, IC50 = 2.14996 μM. SANGER
A253 Growth Inhibition Assay Inhibition of human A253 cell growth in a cell viability assay, IC50 = 2.25377 μM. SANGER
DMS-79 Growth Inhibition Assay Inhibition of human DMS-79 cell growth in a cell viability assay, IC50 = 2.36386 μM. SANGER
SK-N-FI Growth Inhibition Assay Inhibition of human SK-N-FI cell growth in a cell viability assay, IC50 = 2.50034 μM. SANGER
SK-MEL-1 Growth Inhibition Assay Inhibition of human SK-MEL-1 cell growth in a cell viability assay, IC50 = 2.61066 μM. SANGER
HuO-3N1 Growth Inhibition Assay Inhibition of human HuO-3N1 cell growth in a cell viability assay, IC50 = 2.69128 μM. SANGER
SIMA Growth Inhibition Assay Inhibition of human SIMA cell growth in a cell viability assay, IC50 = 2.72143 μM. SANGER
SU-DHL-1 Growth Inhibition Assay Inhibition of human SU-DHL-1 cell growth in a cell viability assay, IC50 = 2.90246 μM. SANGER
P31-FUJ Growth Inhibition Assay Inhibition of human P31-FUJ cell growth in a cell viability assay, IC50 = 3.01209 μM. SANGER
A101D Growth Inhibition Assay Inhibition of human A101D cell growth in a cell viability assay, IC50 = 3.23245 μM. SANGER
RPMI-8866 Growth Inhibition Assay Inhibition of human RPMI-8866 cell growth in a cell viability assay, IC50 = 3.58797 μM. SANGER
NCI-H209 Growth Inhibition Assay Inhibition of human NCI-H209 cell growth in a cell viability assay, IC50 = 3.59247 μM. SANGER
JVM-2 Growth Inhibition Assay Inhibition of human JVM-2 cell growth in a cell viability assay, IC50 = 3.62244 μM. SANGER
LP-1 Growth Inhibition Assay Inhibition of human LP-1 cell growth in a cell viability assay, IC50 = 3.79042 μM. SANGER
IST-SL1 Growth Inhibition Assay Inhibition of human IST-SL1 cell growth in a cell viability assay, IC50 = 3.91611 μM. SANGER
TE-9 Growth Inhibition Assay Inhibition of human TE-9 cell growth in a cell viability assay, IC50 = 3.99619 μM. SANGER
NCI-H1618 Growth Inhibition Assay Inhibition of human NCI-H1618 cell growth in a cell viability assay, IC50 = 4.17788 μM. SANGER
COR-L105 Growth Inhibition Assay Inhibition of human COR-L105 cell growth in a cell viability assay, IC50 = 4.51462 μM. SANGER
KU-19-19 Growth Inhibition Assay Inhibition of human KU-19-19 cell growth in a cell viability assay, IC50 = 4.56729 μM. SANGER
HCC70 Growth Inhibition Assay Inhibition of human HCC70 cell growth in a cell viability assay, IC50 = 5.75167 μM. SANGER
DG-75 Growth Inhibition Assay Inhibition of human DG-75 cell growth in a cell viability assay, IC50 = 6.18885 μM. SANGER
CCF-STTG1 Growth Inhibition Assay Inhibition of human CCF-STTG1 cell growth in a cell viability assay, IC50 = 6.74902 μM. SANGER
SBC-1 Growth Inhibition Assay Inhibition of human SBC-1 cell growth in a cell viability assay, IC50 = 6.88808 μM. SANGER
CAL-85-1 Growth Inhibition Assay Inhibition of human CAL-85-1 cell growth in a cell viability assay, IC50 = 7.45587 μM. SANGER
BxPC-3 Growth Inhibition Assay Inhibition of human BxPC-3 cell growth in a cell viability assay, IC50 = 7.56085 μM. SANGER
YAPC Growth Inhibition Assay Inhibition of human YAPC cell growth in a cell viability assay, IC50 = 7.74286 μM. SANGER
HCC1806 Growth Inhibition Assay Inhibition of human HCC1806 cell growth in a cell viability assay, IC50 = 8.04507 μM. SANGER
SK-MEL-28 Growth Inhibition Assay Inhibition of human SK-MEL-28 cell growth in a cell viability assay, IC50 = 8.42441 μM. SANGER
Ca9-22 Growth Inhibition Assay Inhibition of human Ca9-22 cell growth in a cell viability assay, IC50 = 8.62748 μM. SANGER
NCI-H889 Growth Inhibition Assay Inhibition of human NCI-H889 cell growth in a cell viability assay, IC50 = 9.42457 μM. SANGER
HT-3 Growth Inhibition Assay Inhibition of human HT-3 cell growth in a cell viability assay, IC50 = 9.4754 μM. SANGER
H4 Growth Inhibition Assay Inhibition of human H4 cell growth in a cell viability assay, IC50 = 9.59864 μM. SANGER
NCI-H2228 Growth Inhibition Assay Inhibition of human NCI-H2228 cell growth in a cell viability assay, IC50 = 9.87581 μM. SANGER
M14 Growth Inhibition Assay Inhibition of human M14 cell growth in a cell viability assay, IC50 = 10.5224 μM. SANGER
OVCAR-3 Growth Inhibition Assay Inhibition of human OVCAR-3 cell growth in a cell viability assay, IC50 = 11.4748 μM. SANGER
HCC1187 Growth Inhibition Assay Inhibition of human HCC1187 cell growth in a cell viability assay, IC50 = 11.4776 μM. SANGER
RT4 Growth Inhibition Assay Inhibition of human RT4 cell growth in a cell viability assay, IC50 = 11.6532 μM. SANGER
SW962 Growth Inhibition Assay Inhibition of human SW962 cell growth in a cell viability assay, IC50 = 12.004 μM. SANGER
BB49-HNC Growth Inhibition Assay Inhibition of human BB49-HNC cell growth in a cell viability assay, IC50 = 12.0436 μM. SANGER
HCE-4 Growth Inhibition Assay Inhibition of human HCE-4 cell growth in a cell viability assay, IC50 = 12.7585 μM. SANGER
KU812 Growth Inhibition Assay Inhibition of human KU812 cell growth in a cell viability assay, IC50 = 13.9562 μM. SANGER
JAR Growth Inhibition Assay Inhibition of human JAR cell growth in a cell viability assay, IC50 = 14.113 μM. SANGER
NCI-H2009 Growth Inhibition Assay Inhibition of human NCI-H2009 cell growth in a cell viability assay, IC50 = 14.287 μM. SANGER
HuO9 Growth Inhibition Assay Inhibition of human HuO9 cell growth in a cell viability assay, IC50 = 14.4446 μM. SANGER
U-118-MG Growth Inhibition Assay Inhibition of human U-118-MG cell growth in a cell viability assay, IC50 = 14.5013 μM. SANGER
SH-4 Growth Inhibition Assay Inhibition of human SH-4 cell growth in a cell viability assay, IC50 = 14.942 μM. SANGER
ES5 Growth Inhibition Assay Inhibition of human ES5 cell growth in a cell viability assay, IC50 = 15.3528 μM. SANGER
NB6 Growth Inhibition Assay Inhibition of human NB6 cell growth in a cell viability assay, IC50 = 15.4452 μM. SANGER
COLO-829 Growth Inhibition Assay Inhibition of human COLO-829 cell growth in a cell viability assay, IC50 = 15.6978 μM. SANGER
NCI-H524 Growth Inhibition Assay Inhibition of human NCI-H524 cell growth in a cell viability assay, IC50 = 15.751 μM. SANGER
HPAF-II Growth Inhibition Assay Inhibition of human HPAF-II cell growth in a cell viability assay, IC50 = 16.1017 μM. SANGER
GB-1 Growth Inhibition Assay Inhibition of human GB-1 cell growth in a cell viability assay, IC50 = 16.5577 μM. SANGER
LB771-HNC Growth Inhibition Assay Inhibition of human LB771-HNC cell growth in a cell viability assay, IC50 = 16.8228 μM. SANGER
HuCCT1 Growth Inhibition Assay Inhibition of human HuCCT1 cell growth in a cell viability assay, IC50 = 17.176 μM. SANGER
SW837 Growth Inhibition Assay Inhibition of human SW837 cell growth in a cell viability assay, IC50 = 17.3011 μM. SANGER
NCI-N87 Growth Inhibition Assay Inhibition of human NCI-N87 cell growth in a cell viability assay, IC50 = 17.5302 μM. SANGER
BEN Growth Inhibition Assay Inhibition of human BEN cell growth in a cell viability assay, IC50 = 17.7386 μM. SANGER
Mewo Growth Inhibition Assay Inhibition of human Mewo cell growth in a cell viability assay, IC50 = 17.8382 μM. SANGER
MPP-89 Growth Inhibition Assay Inhibition of human MPP-89 cell growth in a cell viability assay, IC50 = 18.2784 μM. SANGER
NOS-1 Growth Inhibition Assay Inhibition of human NOS-1 cell growth in a cell viability assay, IC50 = 18.3793 μM. SANGER
Ca-Ski Growth Inhibition Assay Inhibition of human Ca-Ski cell growth in a cell viability assay, IC50 = 19.7889 μM. SANGER
SW1088 Growth Inhibition Assay Inhibition of human SW1088 cell growth in a cell viability assay, IC50 = 19.9447 μM. SANGER
Capan-2 Growth Inhibition Assay Inhibition of human Capan-2 cell growth in a cell viability assay, IC50 = 20.3327 μM. SANGER
A498 Growth Inhibition Assay Inhibition of human A498 cell growth in a cell viability assay, IC50 = 20.4092 μM. SANGER
COLO-800 Growth Inhibition Assay Inhibition of human COLO-800 cell growth in a cell viability assay, IC50 = 20.9232 μM. SANGER
OVCAR-4 Growth Inhibition Assay Inhibition of human OVCAR-4 cell growth in a cell viability assay, IC50 = 21.2508 μM. SANGER
K-562 Growth Inhibition Assay Inhibition of human K-562 cell growth in a cell viability assay, IC50 = 21.9049 μM. SANGER
IA-LM Growth Inhibition Assay Inhibition of human IA-LM cell growth in a cell viability assay, IC50 = 22.3771 μM. SANGER
SK-LMS-1 Growth Inhibition Assay Inhibition of human SK-LMS-1 cell growth in a cell viability assay, IC50 = 22.4057 μM. SANGER
CAL-51 Growth Inhibition Assay Inhibition of human CAL-51 cell growth in a cell viability assay, IC50 = 23.0872 μM. SANGER
NCI-H1651 Growth Inhibition Assay Inhibition of human NCI-H1651 cell growth in a cell viability assay, IC50 = 24.1303 μM. SANGER
GMS-10 Growth Inhibition Assay Inhibition of human GMS-10 cell growth in a cell viability assay, IC50 = 24.4376 μM. SANGER
NCI-H2347 Growth Inhibition Assay Inhibition of human NCI-H2347 cell growth in a cell viability assay, IC50 = 24.6005 μM. SANGER
MEL-JUSO Growth Inhibition Assay Inhibition of human MEL-JUSO cell growth in a cell viability assay, IC50 = 24.6906 μM. SANGER
SW948 Growth Inhibition Assay Inhibition of human SW948 cell growth in a cell viability assay, IC50 = 24.9296 μM. SANGER
MKN45 Growth Inhibition Assay Inhibition of human MKN45 cell growth in a cell viability assay, IC50 = 26.4465 μM. SANGER
HCC1954 Growth Inhibition Assay Inhibition of human HCC1954 cell growth in a cell viability assay, IC50 = 26.4502 μM. SANGER
SAS Growth Inhibition Assay Inhibition of human SAS cell growth in a cell viability assay, IC50 = 26.4732 μM. SANGER
SCC-25 Growth Inhibition Assay Inhibition of human SCC-25 cell growth in a cell viability assay, IC50 = 26.5693 μM. SANGER
MKN28 Growth Inhibition Assay Inhibition of human MKN28 cell growth in a cell viability assay, IC50 = 26.7093 μM. SANGER
T84 Growth Inhibition Assay Inhibition of human T84 cell growth in a cell viability assay, IC50 = 26.7743 μM. SANGER
HCT-15 Growth Inhibition Assay Inhibition of human HCT-15 cell growth in a cell viability assay, IC50 = 26.9389 μM. SANGER
DOK Growth Inhibition Assay Inhibition of human DOK cell growth in a cell viability assay, IC50 = 27.0723 μM. SANGER
SK-MEL-24 Growth Inhibition Assay Inhibition of human SK-MEL-24 cell growth in a cell viability assay, IC50 = 28.0766 μM. SANGER
HCC1143 Growth Inhibition Assay Inhibition of human HCC1143 cell growth in a cell viability assay, IC50 = 28.2552 μM. SANGER
JEG-3 Growth Inhibition Assay Inhibition of human JEG-3 cell growth in a cell viability assay, IC50 = 28.4578 μM. SANGER
SF539 Growth Inhibition Assay Inhibition of human SF539 cell growth in a cell viability assay, IC50 = 28.6049 μM. SANGER
NB12 Growth Inhibition Assay Inhibition of human NB12 cell growth in a cell viability assay, IC50 = 28.8033 μM. SANGER
Hs-578-T Growth Inhibition Assay Inhibition of human Hs-578-T cell growth in a cell viability assay, IC50 = 29.1197 μM. SANGER
HCT-116 Growth Inhibition Assay Inhibition of human HCT-116 cell growth in a cell viability assay, IC50 = 29.4423 μM. SANGER
EW-3 Growth Inhibition Assay Inhibition of human EW-3 cell growth in a cell viability assay, IC50 = 29.5269 μM. SANGER
639-V Growth Inhibition Assay Inhibition of human 639-V cell growth in a cell viability assay, IC50 = 29.8546 μM. SANGER
IGR-1 Growth Inhibition Assay Inhibition of human IGR-1 cell growth in a cell viability assay, IC50 = 30.0191 μM. SANGER
LS-411N Growth Inhibition Assay Inhibition of human LS-411N cell growth in a cell viability assay, IC50 = 30.351 μM. SANGER
D-502MG Growth Inhibition Assay Inhibition of human D-502MG cell growth in a cell viability assay, IC50 = 30.3725 μM. SANGER
AN3-CA Growth Inhibition Assay Inhibition of human AN3-CA cell growth in a cell viability assay, IC50 = 31.4329 μM. SANGER
RCC10RGB Growth Inhibition Assay Inhibition of human RCC10RGB cell growth in a cell viability assay, IC50 = 31.4464 μM. SANGER
GT3TKB Growth Inhibition Assay Inhibition of human GT3TKB cell growth in a cell viability assay, IC50 = 31.7096 μM. SANGER
BB30-HNC Growth Inhibition Assay Inhibition of human BB30-HNC cell growth in a cell viability assay, IC50 = 32.5217 μM. SANGER
HT-144 Growth Inhibition Assay Inhibition of human HT-144 cell growth in a cell viability assay, IC50 = 32.7065 μM. SANGER
NCI-H1355 Growth Inhibition Assay Inhibition of human NCI-H1355 cell growth in a cell viability assay, IC50 = 33.3495 μM. SANGER
D-542MG Growth Inhibition Assay Inhibition of human D-542MG cell growth in a cell viability assay, IC50 = 33.3722 μM. SANGER
NCI-H727 Growth Inhibition Assay Inhibition of human NCI-H727 cell growth in a cell viability assay, IC50 = 33.4114 μM. SANGER
NCI-H1755 Growth Inhibition Assay Inhibition of human NCI-H1755 cell growth in a cell viability assay, IC50 = 33.5236 μM. SANGER
KS-1 Growth Inhibition Assay Inhibition of human KS-1 cell growth in a cell viability assay, IC50 = 33.6298 μM. SANGER
SW626 Growth Inhibition Assay Inhibition of human SW626 cell growth in a cell viability assay, IC50 = 33.7643 μM. SANGER
MZ1-PC Growth Inhibition Assay Inhibition of human MZ1-PC cell growth in a cell viability assay, IC50 = 33.8103 μM. SANGER
NCI-H1650 Growth Inhibition Assay Inhibition of human NCI-H1650 cell growth in a cell viability assay, IC50 = 34.326 μM. SANGER
LCLC-97TM1 Growth Inhibition Assay Inhibition of human LCLC-97TM1 cell growth in a cell viability assay, IC50 = 34.8526 μM. SANGER
HTC-C3 Growth Inhibition Assay Inhibition of human HTC-C3 cell growth in a cell viability assay, IC50 = 35.022 μM. SANGER
SNU-387 Growth Inhibition Assay Inhibition of human SNU-387 cell growth in a cell viability assay, IC50 = 35.2684 μM. SANGER
IM-9 Growth Inhibition Assay Inhibition of human IM-9 cell growth in a cell viability assay, IC50 = 35.5165 μM. SANGER
KYSE-180 Growth Inhibition Assay Inhibition of human KYSE-180 cell growth in a cell viability assay, IC50 = 35.6521 μM. SANGER
HuH-7 Growth Inhibition Assay Inhibition of human HuH-7 cell growth in a cell viability assay, IC50 = 35.6756 μM. SANGER
CP66-MEL Growth Inhibition Assay Inhibition of human CP66-MEL cell growth in a cell viability assay, IC50 = 35.7968 μM. SANGER
22RV1 Growth Inhibition Assay Inhibition of human 22RV1 cell growth in a cell viability assay, IC50 = 35.8423 μM. SANGER
SW48 Growth Inhibition Assay Inhibition of human SW48 cell growth in a cell viability assay, IC50 = 36.2464 μM. SANGER
COLO-205 Growth Inhibition Assay Inhibition of human COLO-205 cell growth in a cell viability assay, IC50 = 37.2589 μM. SANGER
MFH-ino Growth Inhibition Assay Inhibition of human MFH-ino cell growth in a cell viability assay, IC50 = 37.3918 μM. SANGER
ECC12 Growth Inhibition Assay Inhibition of human ECC12 cell growth in a cell viability assay, IC50 = 37.7142 μM. SANGER
CAL-72 Growth Inhibition Assay Inhibition of human CAL-72 cell growth in a cell viability assay, IC50 = 37.7221 μM. SANGER
KNS-42 Growth Inhibition Assay Inhibition of human KNS-42 cell growth in a cell viability assay, IC50 = 37.8215 μM. SANGER
U031 Growth Inhibition Assay Inhibition of human U031 cell growth in a cell viability assay, IC50 = 37.827 μM. SANGER
EFE-184 Growth Inhibition Assay Inhibition of human EFE-184 cell growth in a cell viability assay, IC50 = 37.8863 μM. SANGER
TCCSUP Growth Inhibition Assay Inhibition of human TCCSUP cell growth in a cell viability assay, IC50 = 38.3341 μM. SANGER
TE-11 Growth Inhibition Assay Inhibition of human TE-11 cell growth in a cell viability assay, IC50 = 39.2614 μM. SANGER
SK-MEL-3 Growth Inhibition Assay Inhibition of human SK-MEL-3 cell growth in a cell viability assay, IC50 = 39.5577 μM. SANGER
NCI-H1703 Growth Inhibition Assay Inhibition of human NCI-H1703 cell growth in a cell viability assay, IC50 = 39.5618 μM. SANGER
NCI-H748 Growth Inhibition Assay Inhibition of human NCI-H748 cell growth in a cell viability assay, IC50 = 39.6102 μM. SANGER
HuP-T3 Growth Inhibition Assay Inhibition of human HuP-T3 cell growth in a cell viability assay, IC50 = 39.8492 μM. SANGER
GCT Growth Inhibition Assay Inhibition of human GCT cell growth in a cell viability assay, IC50 = 40.2935 μM. SANGER
LU-99A Growth Inhibition Assay Inhibition of human LU-99A cell growth in a cell viability assay, IC50 = 40.6473 μM. SANGER
TGBC24TKB Growth Inhibition Assay Inhibition of human TGBC24TKB cell growth in a cell viability assay, IC50 = 40.7998 μM. SANGER
NCI-H1993 Growth Inhibition Assay Inhibition of human NCI-H1993 cell growth in a cell viability assay, IC50 = 41.1086 μM. SANGER
769-P Growth Inhibition Assay Inhibition of human 769-P cell growth in a cell viability assay, IC50 = 41.226 μM. SANGER
NCI-H2126 Growth Inhibition Assay Inhibition of human NCI-H2126 cell growth in a cell viability assay, IC50 = 41.3099 μM. SANGER
NCI-H1563 Growth Inhibition Assay Inhibition of human NCI-H1563 cell growth in a cell viability assay, IC50 = 41.353 μM. SANGER
NCI-H82 Growth Inhibition Assay Inhibition of human NCI-H82 cell growth in a cell viability assay, IC50 = 41.4039 μM. SANGER
SW1783 Growth Inhibition Assay Inhibition of human SW1783 cell growth in a cell viability assay, IC50 = 41.6769 μM. SANGER
NCI-H1648 Growth Inhibition Assay Inhibition of human NCI-H1648 cell growth in a cell viability assay, IC50 = 41.9392 μM. SANGER
A388 Growth Inhibition Assay Inhibition of human A388 cell growth in a cell viability assay, IC50 = 42.1236 μM. SANGER
SK-MEL-30 Growth Inhibition Assay Inhibition of human SK-MEL-30 cell growth in a cell viability assay, IC50 = 42.3151 μM. SANGER
EKVX Growth Inhibition Assay Inhibition of human EKVX cell growth in a cell viability assay, IC50 = 42.3726 μM. SANGER
A172 Growth Inhibition Assay Inhibition of human A172 cell growth in a cell viability assay, IC50 = 42.8624 μM. SANGER
OMC-1 Growth Inhibition Assay Inhibition of human OMC-1 cell growth in a cell viability assay, IC50 = 42.9399 μM. SANGER
PANC-03-27 Growth Inhibition Assay Inhibition of human PANC-03-27 cell growth in a cell viability assay, IC50 = 43.157 μM. SANGER
NCI-H747 Growth Inhibition Assay Inhibition of human NCI-H747 cell growth in a cell viability assay, IC50 = 43.3223 μM. SANGER
HT55 Growth Inhibition Assay Inhibition of human HT55 cell growth in a cell viability assay, IC50 = 43.7863 μM. SANGER
BV-173 Growth Inhibition Assay Inhibition of human BV-173 cell growth in a cell viability assay, IC50 = 43.7946 μM. SANGER
LS-1034 Growth Inhibition Assay Inhibition of human LS-1034 cell growth in a cell viability assay, IC50 = 43.9899 μM. SANGER
NUGC-3 Growth Inhibition Assay Inhibition of human NUGC-3 cell growth in a cell viability assay, IC50 = 44.0773 μM. SANGER
IST-MEL1 Growth Inhibition Assay Inhibition of human IST-MEL1 cell growth in a cell viability assay, IC50 = 44.2633 μM. SANGER
8305C Growth Inhibition Assay Inhibition of human 8305C cell growth in a cell viability assay, IC50 = 44.31 μM. SANGER
IMR-5 Growth Inhibition Assay Inhibition of human IMR-5 cell growth in a cell viability assay, IC50 = 44.5743 μM. SANGER
TE-6 Growth Inhibition Assay Inhibition of human TE-6 cell growth in a cell viability assay, IC50 = 44.8539 μM. SANGER
MZ2-MEL Growth Inhibition Assay Inhibition of human MZ2-MEL cell growth in a cell viability assay, IC50 = 45.0112 μM. SANGER
RT-112 Growth Inhibition Assay Inhibition of human RT-112 cell growth in a cell viability assay, IC50 = 45.4472 μM. SANGER
SCC-9 Growth Inhibition Assay Inhibition of human SCC-9 cell growth in a cell viability assay, IC50 = 45.5616 μM. SANGER
SCC-4 Growth Inhibition Assay Inhibition of human SCC-4 cell growth in a cell viability assay, IC50 = 45.5626 μM. SANGER
CAL-54 Growth Inhibition Assay Inhibition of human CAL-54 cell growth in a cell viability assay, IC50 = 45.6635 μM. SANGER
LoVo Growth Inhibition Assay Inhibition of human LoVo cell growth in a cell viability assay, IC50 = 45.6839 μM. SANGER
NCI-H358 Growth Inhibition Assay Inhibition of human NCI-H358 cell growth in a cell viability assay, IC50 = 45.7286 μM. SANGER
K5 Growth Inhibition Assay Inhibition of human K5 cell growth in a cell viability assay, IC50 = 46.029 μM. SANGER
LB1047-RCC Growth Inhibition Assay Inhibition of human LB1047-RCC cell growth in a cell viability assay, IC50 = 46.4256 μM. SANGER
MES-SA Growth Inhibition Assay Inhibition of human MES-SA cell growth in a cell viability assay, IC50 = 46.5121 μM. SANGER
UACC-257 Growth Inhibition Assay Inhibition of human UACC-257 cell growth in a cell viability assay, IC50 = 46.5175 μM. SANGER
NCI-H2342 Growth Inhibition Assay Inhibition of human NCI-H2342 cell growth in a cell viability assay, IC50 = 46.8809 μM. SANGER
TE-15 Growth Inhibition Assay Inhibition of human TE-15 cell growth in a cell viability assay, IC50 = 46.8909 μM. SANGER
SK-MES-1 Growth Inhibition Assay Inhibition of human SK-MES-1 cell growth in a cell viability assay, IC50 = 47.3821 μM. SANGER
NCI-H322M Growth Inhibition Assay Inhibition of human NCI-H322M cell growth in a cell viability assay, IC50 = 47.6561 μM. SANGER
SK-OV-3 Growth Inhibition Assay Inhibition of human SK-OV-3 cell growth in a cell viability assay, IC50 = 47.7452 μM. SANGER
PANC-08-13 Growth Inhibition Assay Inhibition of human PANC-08-13 cell growth in a cell viability assay, IC50 = 47.9641 μM. SANGER
786-0 Growth Inhibition Assay Inhibition of human 786-0 cell growth in a cell viability assay, IC50 = 48.073 μM. SANGER
NCI-H460 Growth Inhibition Assay Inhibition of human NCI-H460 cell growth in a cell viability assay, IC50 = 48.1255 μM. SANGER
D-336MG Growth Inhibition Assay Inhibition of human D-336MG cell growth in a cell viability assay, IC50 = 48.4845 μM. SANGER
SNG-M Growth Inhibition Assay Inhibition of human SNG-M cell growth in a cell viability assay, IC50 = 48.6701 μM. SANGER
SK-CO-1 Growth Inhibition Assay Inhibition of human SK-CO-1 cell growth in a cell viability assay, IC50 = 49.0025 μM. SANGER
CAL-27 Growth Inhibition Assay Inhibition of human CAL-27 cell growth in a cell viability assay, IC50 = 49.5334 μM. SANGER
8505C Growth Inhibition Assay Inhibition of human 8505C cell growth in a cell viability assay, IC50 = 49.5364 μM. SANGER
MCF7 Growth Inhibition Assay Inhibition of human MCF7 cell growth in a cell viability assay, IC50 = 49.5865 μM. SANGER
T47D Growth Inhibition Assay Inhibition of human T47D cell growth in a cell viability assay, IC50 = 49.7007 μM. SANGER
YKG-1 Growth Inhibition Assay Inhibition of human YKG-1 cell growth in a cell viability assay, IC50 = 49.9412 μM. SANGER
Click to View More Cell Line Experimental Data

Biological Activity

Description A-769662 is a potent, reversible AMPK activator with EC50 of 0.8 μM in cell-free assays, little effect on GPPase/FBPase activity.
Targets
AMPK [1]
(Cell-free assay)		 Fatty acid synthesis [1]
(in primary rat hepatocytes)
0.8 μM(EC50) 3.2 μM
In vitro
In vitro

A-769662 stimulates partially purified rat liver AMPK with EC50 with 0.8 μM. A-769662 activates AMPK purified from multiple tissues and species in a dose-responsive manner with modest variations in observed EC50s. EC50s determined for A-769662 using partially purified AMPK extracts from rat heart, rat muscle, or human embryonic kidney cells (HEKs) are 2.2 mM, 1.9 mM, or 1.1 mM, respectively. A 4 hours treatment of primary rat hepatocytes with A-769662 dose-dependently increases ACC phosphorylation, which correlated inhibition of fatty acid synthesis with IC50 of 3.2 μM. A-769662 also inhibits fatty acid sythesis in mouse hepatocytes with IC50 with 3.6 μM [1] A-769662 activates AMPK both allosterically and by inhibiting dephosphorylation of AMPK on Thr-172, similar to the effects of AMP. [2] A-769662 inhibits proteasomal function by an AMPK-independent mechanism. A-769662 affects the in vitro activity of purified 26S proteasomes but not the in vitro activity of purified 20S proteasomes. A-769662 has toxic effects on MEF cells. [3] A recent research shows A-769662 inhibited cell proliferation and DNA synthesis. [4]
Kinase Assay 96-well AMPK assay
AMPK activity is measured by monitoring phosphorylation of the SAMS peptide substrate (20 μM in standard assays and 100 μM in additivity assays) following a previously described protocol (Anderson et al., 2004). To determine whether A-769662-induced AMPK activation occurs in a reversible manner, AMP or A-769662 are preincubated with rat liver AMPK for 10 minutes at 20 times standard assay concentrations prior to dilution and measurement of AMPK activity.

[1]
Cell Research Cell lines MEF cells
Concentrations 300 μM
Incubation Time 24 hours
Method

Cell viability of MEF cells treated or not with A-769662 is performed as follows: cells are harvested by trypsinization and incubated with 0.5 mg/mL RNase and 50 μg/mL propidium iodine at room temperature in the dark; cell viability is analyzed by flow cytometry using a FACScanto flow cytometer, using an excitation laser at 488 nm and a propidium iodine fluorescence detection at 600 nm. To determine the proportion of cells in each phase of the cell cycle, cells are harvested by trypsinization, collected by centrifugation, washed in PBS and fixed overnight in 80% ethanol at -20 °C. Subsequently, these fixed cells are centrifuged to remove the fixative and incubated for 20 minutes in the dark at room temperature in PBS containing 0.5 mg/mL RNase and 50 μg/mL propidium iodine. Flow cytometry analysis is performed as above. The proportion of cells in G1, S, and G2 is determined using the MODFIT program. Cell culture pictures are taken at the indicated times using a camera coupled to an inverted microscope with a 20 × objective.
Experimental Result Images Methods Biomarkers Images PMID
Western blot pCofilin / Cofilin / detyrosinated alpha tubulin / acetylated alpha tubulin p-AMPK / AMPK / p-S6K / S6K / p-ACC 26431377
In Vivo
In vivo

Short-term treatment of normal Sprague Dawley rats with A-769662 decreases liver malonyl CoA levels and the respiratory exchange ratio, VCO2/VO2, indicating an increased rate of whole-body fatty acid oxidation. Treatment of ob/ob mice with 30 mg/kg b.i.d. A-769662 decreases hepatic expression of PEPCK, G6Pase, and FAS, lowers plasma glucose by 40%, reduced body weight gain and significantly decreases both plasma and liver triglyceride levels. [1]
Animal Research Animal Models Sprague Dawley rats
Dosages 30 mg/kg
Administration Administered via i.p.

Chemical Information & Solubility

Molecular Weight 360.39 Formula

C20H12N2O3S
CAS No. 844499-71-4 SDF Download A-769662 SDF
Smiles C1=CC=C(C(=C1)C2=CC=C(C=C2)C3=CSC4=C3C(=C(C(=O)N4)C#N)O)O
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 72 mg/mL ( (199.78 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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