Trans-ferulic acid

Catalog No.E0653 Batch:E065301

Technical Data

Formula	C₁₀H₁₀O₄
Molecular Weight	194.18	CAS No.	537-98-4
Solubility (25°C)*	In vitro


* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Biological Activity

Description

Trans-ferulic acid is a potent activator of AMPKunder high glucose condition.

Targets

AMPK ^[1]

In vitro

Trans-ferulic acid can promote the activation of AMPK and the phosphorylation of acetyl-CoA carboxylase in hyperglycemia-induced human liver cells (HepG2) and rat skeletal muscle cells (L6).Trans-ferulic acid reduces the level of intracellular reactive oxygen species (ROS) and nitric oxide (NO) induced by hyperglycemia and subsequently increases the level of glutathione.^[2]

In vivo

TFA exhibits a potential protective effect againstf cisplatin induced testicular injury by inhibiting oxidative stress as well as TLR4/IRF3/INF-γ, P38-MAPK/NF-κB-p65/TNF-α, and JAK1/STAT-3/ERK1/2 inflammatory signaling pathways.^[2]

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines Human liver cells (HepG2), rat skeletal muscle cells (L6), Concentrations 10, 20, 30 μM Incubation Time 24 h Method Fully-differentiated L6 myotubes and HepG2 cells are sub-cultured in six-well plates at a cell density of 2.5 x 10⁵ cells/well and incubated in fresh DMEM containing 5 mM glucose for the control well and 25 mM glucose for the remaining wells for 24 hr. After incubation, the cells are treated separately in the serum-free media with 10, 20, and 30 μM concentrations of trans-ferulic acid and incubated further for 24 hr. Then, the cell extraction is used for western blot.
Animal Study:^{^[2]}	Animal Models Sprague–Dawley rats Dosages 50 mg/kg Administration p.o.

Cell Assay:^{^[1]}

Cell lines
Human liver cells (HepG2), rat skeletal muscle cells (L6),
Concentrations
10, 20, 30 μM
Incubation Time
24 h
Method
Fully-differentiated L6 myotubes and HepG2 cells are sub-cultured in six-well plates at a cell density of 2.5 x 10⁵ cells/well and incubated in fresh DMEM containing 5 mM glucose for the control well and 25 mM glucose for the remaining wells for 24 hr. After incubation, the cells are treated separately in the serum-free media with 10, 20, and 30 μM concentrations of trans-ferulic acid and incubated further for 24 hr. Then, the cell extraction is used for western blot.

Animal Study:^{^[2]}

Animal Models
Sprague–Dawley rats
Dosages
50 mg/kg
Administration
p.o.

References

RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.

SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.