Home Immunology & Inflammation TLR antagonist TLR4-IN-C34 For research use only.

TLR4-IN-C34

Synonyms: TLR4-C34, C34, TLR4-C34-IN

TLR4-IN-C34 (TLR4-C34, C34, TLR4-C34-IN) is a potent and selective antagonist of Toll-like receptor 4 (TLR4). TLR4-IN-C34 reduces systemic inflammation in mouse models of endotoxemia and necrotizing enterocolitis.

TLR4-IN-C34 Chemical Structure

TLR4-IN-C34 Chemical Structure

CAS No. 40592-88-9

Purity & Quality Control

Batch: S082201 DMSO]78 mg/mL]false]Water]78 mg/mL]false]Ethanol]78 mg/mL]false Purity: 99.74%
99.74

TLR4-IN-C34 Related Products

Biological Activity

Description TLR4-IN-C34 (TLR4-C34, C34, TLR4-C34-IN) is a potent and selective antagonist of Toll-like receptor 4 (TLR4). TLR4-IN-C34 reduces systemic inflammation in mouse models of endotoxemia and necrotizing enterocolitis.
Targets
TLR4 [1]
In vitro
In vitro

TLR4-IN-C34 inhibits TLR4 in vitro in macrophages and enterocytes via direct binding.[1]
Cell Research Cell lines IEC-6 cells, RAW 264.7 cells
Concentrations 10 μM
Incubation Time 30 min
Method

IEC-6 cells and RAW 264.7 cells are treated withTLR4-IN-C34 at a concentration of 10 μM 30 min prior to treatment with LPS, and the extent of LPS signaling is determined by the degree of TNFα expression by qRT-PCR. In parallel, RAW 264.7 cells are transduced with an adenovirus expressing the NFκB-luciferase reporter gene, and then treated with LPS at 10 ng/ml after pre-treatment with TLR4-IN-C34 at 100 µM. The NFκB-luciferase activity is measured using the Luciferase Activity System.
In Vivo
In vivo

TLR4-IN-C34 can attenuate NEC severity and demonstrates a marked preservation of the intestinal mucosa.
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT03020524 Completed
Hiv
University of Pennsylvania
 January 2017 Early Phase 1

Chemical Information & Solubility

Molecular Weight 389.40 Formula

C17H27NO9
CAS No. 40592-88-9 SDF --
Smiles CC(C)OC1C(C(C(C(O1)COC(=O)C)OC(=O)C)OC(=O)C)NC(=O)C
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 78 mg/mL ( (200.3 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 78 mg/mL

Ethanol : 78 mg/mL


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In vivo
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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