TLR4-IN-C34

Catalog No.S0822 Batch:S082201

Technical Data

Formula	C₁₇H₂₇NO₉
Molecular Weight	389.40	CAS No.	40592-88-9
Solubility (25°C)*	In vitro	DMSO	78 mg/mL (200.3 mM)
		Water	78 mg/mL (200.3 mM)
		Ethanol	78 mg/mL (200.3 mM)
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

TLR4-IN-C34 (TLR4-C34, C34, TLR4-C34-IN) is a potent and selective antagonist of Toll-like receptor 4 (TLR4). TLR4-IN-C34 reduces systemic inflammation in mouse models of endotoxemia and necrotizing enterocolitis.

Targets

TLR4 ^[1]

In vitro

TLR4-IN-C34 inhibits TLR4 in vitro in macrophages and enterocytes via direct binding.^[1]

In vivo

TLR4-IN-C34 can attenuate NEC severity and demonstrates a marked preservation of the intestinal mucosa.

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines IEC-6 cells, RAW 264.7 cells Concentrations 10 μM Incubation Time 30 min Method IEC-6 cells and RAW 264.7 cells are treated withTLR4-IN-C34 at a concentration of 10 μM 30 min prior to treatment with LPS, and the extent of LPS signaling is determined by the degree of TNFα expression by qRT-PCR. In parallel, RAW 264.7 cells are transduced with an adenovirus expressing the NFκB-luciferase reporter gene, and then treated with LPS at 10 ng/ml after pre-treatment with TLR4-IN-C34 at 100 µM. The NFκB-luciferase activity is measured using the Luciferase Activity System.

Cell Assay:^{^[1]}

Cell lines
IEC-6 cells, RAW 264.7 cells
Concentrations
10 μM
Incubation Time
30 min
Method
IEC-6 cells and RAW 264.7 cells are treated withTLR4-IN-C34 at a concentration of 10 μM 30 min prior to treatment with LPS, and the extent of LPS signaling is determined by the degree of TNFα expression by qRT-PCR. In parallel, RAW 264.7 cells are transduced with an adenovirus expressing the NFκB-luciferase reporter gene, and then treated with LPS at 10 ng/ml after pre-treatment with TLR4-IN-C34 at 100 µM. The NFκB-luciferase activity is measured using the Luciferase Activity System.

References

Selleck's TLR4-IN-C34 has been cited by 3 publications

Nucleus pulposus cells regulate macrophages in degenerated intervertebral discs via the integrated stress response-mediated CCL2/7-CCR2 signaling pathway [ Exp Mol Med, 2024, 56(2):408-421.]	PubMed: 38316963
Tumor cell-released autophagosomes (TRAPs) induce PD-L1-decorated NETs that suppress T-cell function to promote breast cancer pulmonary metastasis [ J Immunother Cancer, 2024, 12(6)e009082]	PubMed: 38926151
Oxidative stress mediated by the TLR4/NOX2 signalling axis is involved in polystyrene microplastic-induced uterine fibrosis in mice [ Sci Total Environ, 2022, 838(Pt 2):155825]	PubMed: 35597360

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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