Home Immunology & Inflammation TLR inhibitor TLR2-IN-C29 For research use only.

TLR2-IN-C29

Synonyms: C29

TLR2-IN-C29 (C29) is a Toll-like receptor 2 (TLR2) inhibitor that inhibits TLR2/1 and TLR2/6 signaling.

TLR2-IN-C29 Chemical Structure

TLR2-IN-C29 Chemical Structure

CAS No. 363600-92-4

Purity & Quality Control

TLR2-IN-C29 Related Products

Biological Activity

Description TLR2-IN-C29 (C29) is a Toll-like receptor 2 (TLR2) inhibitor that inhibits TLR2/1 and TLR2/6 signaling.
Targets
TLR2 [1]
In vitro
In vitro

TLR2-IN-C29 inhibites TLR2/1 and TLR2/6 signaling induced by synthetic and bacterial TLR2 agonists in human HEK-TLR2 and THP-1 cells, but only TLR2/1 signaling in murine macrophages. TLR2-IN-C29 fails to inhibit signaling induced by other TLR agonists and TNF-α.[2]
Cell Research Cell lines THP-1 cells, HEK-TLR2 cells, murine macrophages
Concentrations 10 μM-150 μM
Incubation Time 5 h
Method

After transfection, cells are allowed to recover for 48 h and treated for 5 h with medium or stimuli in the presence/absence of C29. Cells are lysed in a passive lysis buffer, and firefly luciferase and Renilla luciferase activities are measured using the Dual-Luciferase Reporter Assay System. Cytokine levels in culture supernatants are analyzed by Multiplex beads in the Cytokine Core Laboratory.
In Vivo
In vivo

TLR2-IN-C29 reduces significantly the growth of tumors in Clear cell Renal Cell (RCC) and Head and Neck Squamous Cell Carcinomas (HNSCC) xenograft mice.[3]
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT05998122 Not yet recruiting
Locally Advanced Rectal Cancer|Total Neoadjuvant Treatment|Anti-PD-1
The First Hospital of Jilin University
 September 1 2023 Phase 2
NCT05368168 Recruiting
Rectal Disorders|Low Anterior Resection Syndrome|Low Anterior Resection
Innovacion en Cirugía Vigo
 June 3 2022 Not Applicable
NCT04906044 Active not recruiting
Tislelizumab|Locally Advanced Rectal Cancer|Total Neoadjuvant Treatment|PD-1
The First Hospital of Jilin University
 June 1 2021 Phase 1
NCT05046756 Recruiting
Colon Cancer
Korea University Anam Hospital|National IT Industry Promotion Agency
 May 11 2021 Not Applicable

Chemical Information & Solubility

Molecular Weight 285.29 Formula

C16H15NO4
CAS No. 363600-92-4 SDF --
Smiles CC1=C(C=CC=C1N=CC2=C(C(=CC=C2)OC)O)C(=O)O
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 57 mg/mL ( (199.79 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 6 mg/mL

Water : Insoluble


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In vivo
Batch:

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In vivo Formulation Calculator

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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