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TLR2-IN-C29 TLR2 Inhibitor

Name: TLR2-IN-C29
Brand: Selleck Chemicals
SKU: S6597
Availability: InStock
Rating: 5 (21 reviews)

Cat.No.S6597

TLR2-IN-C29 (C29) is a Toll-like receptor 2 (TLR2) inhibitor that inhibits TLR2/1 and TLR2/6 signaling.

Chemical Structure

Molecular Weight: 285.29

Jump to Mechanism of Action Solubility Chemical Information, Storage & Stability Specificity

Quality Control

Batch: Purity: 99.82%

99.82

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Chemical Information, Storage & Stability

Molecular Weight	285.29	Formula	C₁₆H₁₅NO₄	Storage (From the date of receipt)	3 years -20°C powder
CAS No.	363600-92-4	--		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	C29			Smiles	CC1=C(C=CC=C1N=CC2=C(C(=CC=C2)OC)O)C(=O)O

Solubility

In vitro
Batch:

DMSO : 57 mg/mL (199.79 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : 6 mg/mL

Water : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
=	×	×

Dilution Calculator Molecular Weight Calculator

In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	8.250mg/ml (28.92mM)	Taking the 1 mL working solution as an example, add 50 μL of 165 mg/ml clarified DMSO stock solution to 400 μL PEG300, mix evenly to clarify it; add 50 μL Tween80 to the above system, mix evenly to clarify it; then continue to add 500 μL ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Mechanism of Action

Targets/IC₅₀/Ki	TLR2 ^[1]
In vitro	TLR2-IN-C29 inhibites TLR2/1 and TLR2/6 signaling induced by synthetic and bacterial TLR2 agonists in human HEK-TLR2 and THP-1 cells, but only TLR2/1 signaling in murine macrophages. This compound fails to inhibit signaling induced by other TLR agonists and TNF-α.^[2]
In vivo	TLR2-IN-C29 reduces significantly the growth of tumors in Clear cell Renal Cell (RCC) and Head and Neck Squamous Cell Carcinomas (HNSCC) xenograft mice.^[3]
References	[1] https://pubmed.ncbi.nlm.nih.gov/25870276/ [2] https://pubmed.ncbi.nlm.nih.gov/25870276/ [3] https://pubmed.ncbi.nlm.nih.gov/31410218/

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2024-05-22)

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT05998122	Not yet recruiting	Locally Advanced Rectal Cancer\|Total Neoadjuvant Treatment\|Anti-PD-1	The First Hospital of Jilin University	September 1 2023	Phase 2
NCT05368168	Recruiting	Rectal Disorders\|Low Anterior Resection Syndrome\|Low Anterior Resection	Innovacion en Cirugía Vigo	June 3 2022	Not Applicable
NCT04906044	Active not recruiting	Tislelizumab\|Locally Advanced Rectal Cancer\|Total Neoadjuvant Treatment\|PD-1	The First Hospital of Jilin University	June 1 2021	Phase 1
NCT05046756	Recruiting	Colon Cancer	Korea University Anam Hospital\|National IT Industry Promotion Agency	May 11 2021	Not Applicable

Tech Support

Handling Instructions

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