Home Immunology & Inflammation TLR agonist Vesatolimod (GS-9620) For research use only.

Vesatolimod (GS-9620)

Vesatolimod (GS-9620) is a potent and selective orally active small molecule agonist of Toll-like receptor 7.

Vesatolimod (GS-9620) Chemical Structure

Vesatolimod (GS-9620) Chemical Structure

CAS No. 1228585-88-3

Purity & Quality Control

Batch: S722101 4-Methylpyridine]12 mg/mL]true]DMSO]8.8 mg/mL]true]Water]Insoluble]false Purity: 99.93%
99.93

Vesatolimod (GS-9620) Related Products

Biological Activity

Description Vesatolimod (GS-9620) is a potent and selective orally active small molecule agonist of Toll-like receptor 7.
Targets
TLR7 [1]
In vitro
In vitro GS-9620 potently inhibited viral replication in peripheral blood mononuclear cells(PBMCs), particularly when added 24 to 48 hours prior to HIV infection (EC50= 27 nM). Depletion of pDCs, but not other immune cell subsets from PBMC cultures, suppressed GS-9620 antiviral activity[2].
Cell Research Cell lines Human PBMCs
Concentrations serial dilutions
Incubation Time 5 days
Method

Uninfected PBMCs from individual donors are first activated in bulk using PHA and IL-2 at 37°C for 48 hours and then seeded into 96-well plates at a density of 2 x 105 cells per well before being dosed in triplicate with 4-fold serial dilutions of compounds prepared in RPMI cell culture medium. Cells are incubated in a 5% CO2 incubator at 37°C for a period of 5 days. At the end of the assay,
cell viability is assessed.
In Vivo
In vivo Short-term oral administration of GS-9620 provides long-term suppression of serum and liver HBV DNA. GS-9620 administration induces production of IFN-α and other cytokines and chemokines, and activates ISGs, natural killer cells, and lymphocyte subsets. Its treatment reduces viral DNA, Serum levels of HB surface antigen, HB e antigen and numbers of HBV antigen-positive hepatocytes while hepatocyte apoptosis increases[1]. Currently, GS-9620 is under clinical evaluation for treating chronic HBV infection and for reducing latent reservoirs in virally-suppressed HIV-infected patients[2].
Animal Research Animal Models chimpanzees
Dosages 1 mg/kg and 2 mg/kg
Administration oral administration

Chemical Information & Solubility

Molecular Weight 410.51 Formula

C22H30N6O2
CAS No. 1228585-88-3 SDF Download Vesatolimod (GS-9620) SDF
Smiles CCCCOC1=NC(=C2C(=N1)N(CC(=O)N2)CC3=CC=CC(=C3)CN4CCCC4)N
Storage (From the date of receipt)

In vitro
Batch:

4-Methylpyridine : 12 mg/mL

DMSO : 8.8 mg/mL ( (21.43 mM) Warmed with 50°C water bath; Ultrasonicated; Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble


Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

Mass Concentration Volume Molecular Weight

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy Vesatolimod (GS-9620) | Vesatolimod (GS-9620) supplier | purchase Vesatolimod (GS-9620) | Vesatolimod (GS-9620) cost | Vesatolimod (GS-9620) manufacturer | order Vesatolimod (GS-9620) | Vesatolimod (GS-9620) distributor