Home Immunology & Inflammation TLR inhibitor E6446 dihydrochloride For research use only.

E6446 dihydrochloride

E6446 dihydrochloride is a specific Toll-like receptor 9 inhibitor.

E6446 dihydrochloride Chemical Structure

E6446 dihydrochloride Chemical Structure

CAS No. 1345675-25-3

Purity & Quality Control

E6446 dihydrochloride Related Products

Cell Data

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HEK293 Function assay 24 hrs Antagonist activity at human TLR9 expressed in HEK293 cells assessed as inhibition of CpGB-induced NF-kappaB activation after 24 hrs by spectrophotometry based SEAP reporter gene assay, IC50=0.015μM 28437629
plasmacytoid dendritic cells Function assay 18 hrs Antagonist activity at TLR9 in human plasmacytoid dendritic cells assessed as inhibition of CpGA-induced IFN-alpha production after 18 hrs by ELISA, IC50<0.05μM 28437629
HEK293 Function assay 24 hrs Antagonist activity at human TLR7 expressed in HEK293 cells assessed as inhibition of CL264-induced NF-kappaB activation after 24 hrs by spectrophotometry based SEAP reporter gene assay, IC50=1.99μM 28437629
Click to View More Cell Line Experimental Data

Biological Activity

Description E6446 dihydrochloride is a specific Toll-like receptor 9 inhibitor.
Targets
TLR9 [1]
In Vivo
In vivo

E6446 is orally bioavailable (20%) and has high volumes of distribution in mice (95.9 l/kg)[1].
Animal Research Animal Models MRL/lpr mice
Dosages 20 or 60 mg/kg
Administration oral

Chemical Information & Solubility

Molecular Weight 522.51 Formula

C27H37Cl2N3O3
CAS No. 1345675-25-3 SDF --
Smiles C1CCN(C1)CCCOC2=CC=C(C=C2)C3=NC4=C(O3)C=C(C=C4)OCCCN5CCCC5.Cl.Cl
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

Water : 100 mg/mL

DMSO : 3 mg/mL ( (5.74 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 2 mg/mL


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In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

Preparing Stock Solutions

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Mass Concentration Volume Molecular Weight

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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