TLR2-IN-C29

Catalog No.S6597 Batch:S659704

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Technical Data

Formula

C16H15NO4
Molecular Weight 285.29 CAS No. 363600-92-4
Solubility (25°C)* In vitro DMSO 57 mg/mL (199.79 mM)
Water Insoluble
Ethanol Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description TLR2-IN-C29 (C29) is a Toll-like receptor 2 (TLR2) inhibitor that inhibits TLR2/1 and TLR2/6 signaling.
Targets
TLR2 [1]
In vitro

TLR2-IN-C29 inhibites TLR2/1 and TLR2/6 signaling induced by synthetic and bacterial TLR2 agonists in human HEK-TLR2 and THP-1 cells, but only TLR2/1 signaling in murine macrophages. TLR2-IN-C29 fails to inhibit signaling induced by other TLR agonists and TNF-α.[2]
In vivo

TLR2-IN-C29 reduces significantly the growth of tumors in Clear cell Renal Cell (RCC) and Head and Neck Squamous Cell Carcinomas (HNSCC) xenograft mice.[3]

Protocol (from reference)

Cell Assay:

[2]
  • Cell lines

    THP-1 cells, HEK-TLR2 cells, murine macrophages

  • Concentrations

    10 μM-150 μM

  • Incubation Time

    5 h

  • Method

    After transfection, cells are allowed to recover for 48 h and treated for 5 h with medium or stimuli in the presence/absence of C29. Cells are lysed in a passive lysis buffer, and firefly luciferase and Renilla luciferase activities are measured using the Dual-Luciferase Reporter Assay System. Cytokine levels in culture supernatants are analyzed by Multiplex beads in the Cytokine Core Laboratory.

Selleck's TLR2-IN-C29 has been cited by 11 publications

Tumor cell-released autophagosomes (TRAPs) induce PD-L1-decorated NETs that suppress T-cell function to promote breast cancer pulmonary metastasis [ J Immunother Cancer, 2024, 12(6)e009082] PubMed: 38926151
Streptococcus pneumoniae endopeptidase O induces trained immunity and confers protection against various pathogenic infections [ Clin Immunol, 2024, 263:110226] PubMed: 38663493
Suppressing neutrophil itaconate production attenuates Mycoplasma pneumoniae pneumonia [ PLoS Pathog, 2024, 20(11):e1012614] PubMed: 39499730
Hangeshashinto Inhibits Porphyromonas gingivalis Pathogen-Associated Molecular Patterns-Mediated IL-6 and IL-8 Production through Toll-Like Receptors in CAL27 Cells [ Evid Based Complement Alternat Med, 2024, 2024:9866670] PubMed: 38665937
An invasive zone in human liver cancer identified by Stereo-seq promotes hepatocyte-tumor cell crosstalk, local immunosuppression and tumor progression [ Cell Res, 2023, 33(8):585-603] PubMed: 37337030
Dual RNA-Sequencing and Liquid Chromatography-Mass Spectrometry Unveil Specific Insights on the Pathogenicity of Trichophyton mentagrophytes Complex [ J Invest Dermatol, 2023, 143(3):470-479.e6] PubMed: 38295003
Acrylamide induces the activation of BV2 microglial cells through TLR2/4-mediated LRRK2-NFATc2 signaling cascade [ Food Chem Toxicol, 2023, 176:113775] PubMed: 37037409
Acrylamide induces the activation of BV2 microglial cells through TLR2/4-mediated LRRK2-NFATc2 signaling cascade [ Food Chem Toxicol, 2023, 176:113775] PubMed: 37037409
Airway Prevotella promote TLR2-dependent neutrophil activation and rapid clearance of Streptococcus pneumoniae from the lung [ Nat Commun, 2022, 13(1):3321] PubMed: 35680890
Distinct activities of Vδ1+ T-cells upon different cytomegalovirus reactivation status after haematopoietic transplantation [ Immunology, 2022, 167(3):368-383] PubMed: 35795896

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.