Home Apoptosis MDM2/MDMX inhibitor SJ-172550 For research use only.

SJ-172550

SJ 172550 is the first MDMX inhibitor that binds reversibly to MDMX and effectively kills retinoblastoma cells in which the expression of MDMX is amplified.

SJ-172550 Chemical Structure

SJ-172550 Chemical Structure

CAS No. 431979-47-4

Purity & Quality Control

Batch: E005101 DMSO]86 mg/mL]false]Water]Insoluble]false]Ethanol]Insoluble]false Purity: 99.13%
99.13

SJ-172550 Related Products

Signaling Pathway

MDM2/MDMX Signaling Pathways

MDM2/MDMX Signaling Pathway

Biological Activity

Description SJ 172550 is the first MDMX inhibitor that binds reversibly to MDMX and effectively kills retinoblastoma cells in which the expression of MDMX is amplified.
Targets
MDMX [1]
In vitro
In vitro

SJ-172550 binds reversibly to MDMX and effectively kills retinoblastoma cells in which the expression of MDMX is amplified. The effect of SJ-172550 is additive when combined with an MDM2 inhibitor nutlin-3a.[1]
Cell Research Cell lines Retinoblastoma cells, ML-1 leukemia cells
Concentrations 20 μM
Incubation Time 20 h
Method

Retinoblastoma cells and ML-1 leukemia cells (with wild-type p53) are exposed to SJ-172550 (20 μm) for 20 h. And then the p53 and activated caspase-3 levels are analyzed by immunofluorescence. Nutlin-3a (5 μm; 20 h) is used for as the positive controls. DMSO is used as the negative control.

Chemical Information & Solubility

Molecular Weight 428.87 Formula

C₂₂H₂₁ClN₂O₅
CAS No. 431979-47-4 SDF --
Smiles CCOC1=C(OCC(=O)OC)C(=CC(=C1)\C=C2/C(=NN(C2=O)C3=CC=CC=C3)C)Cl
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 86 mg/mL ( (200.52 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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