Jurkat |
Function assay |
|
|
|
Inhibition of CK2 in human Jurkat cells assessed as inhibition of [gamma33P]ATP incorporation into substrate by luminescence assay, IC50=0.1μM |
21174434 |
MIAPaCa2 |
Antiproliferative assay |
|
4 days |
|
Antiproliferative activity against human MIAPaCa2 cells after 4 days by alamar blue assay, IC50=1.1μM |
21174434 |
PC3 |
Antiproliferative assay |
|
4 days |
|
Antiproliferative activity against human PC3 cells after 4 days by alamar blue assay, IC50=2.1μM |
21174434 |
HCT116 |
Antiproliferative assay |
|
4 days |
|
Antiproliferative activity against human HCT116 cells after 4 days by alamar blue assay, IC50=2.2μM |
21174434 |
H1299 |
Antiproliferative assay |
|
4 days |
|
Antiproliferative activity against human H1299 cells after 4 days by alamar blue assay, IC50=2.4μM |
21174434 |
Jurkat |
Antiproliferative assay |
|
4 days |
|
Antiproliferative activity against human Jurkat cells after 4 days by alamar blue assay, IC50=2.5μM |
21174434 |
A549 |
Antiproliferative assay |
|
4 days |
|
Antiproliferative activity against human A549 cells after 4 days by alamar blue assay, IC50=3μM |
21174434 |
A375 |
Antiproliferative assay |
|
4 days |
|
Antiproliferative activity against human A375 cells after 4 days by alamar blue assay, IC50=3.9μM |
21174434 |
BxPC3 |
Antiproliferative assay |
|
4 days |
|
Antiproliferative activity against human BxPC3 cells after 4 days by alamar blue assay, IC50=4.4μM |
21174434 |
LNCAP |
Antiproliferative assay |
|
4 days |
|
Antiproliferative activity against human LNCAP cells after 4 days by alamar blue assay, IC50=4.7μM |
21174434 |
K562 |
Antiproliferative assay |
|
4 days |
|
Antiproliferative activity against human K562 cells after 4 days by alamar blue assay, IC50=5.3μM |
21174434 |
MDA-MB-231 |
Antiproliferative assay |
|
4 days |
|
Antiproliferative activity against human MDA-MB-231 cells after 4 days by alamar blue assay, IC50=6.4μM |
21174434 |
MCF7 |
Antiproliferative assay |
|
4 days |
|
Antiproliferative activity against human MCF7 cells after 4 days by alamar blue assay, IC50=8.9μM |
21174434 |
Hs 578T |
Antiproliferative assay |
|
4 days |
|
Antiproliferative activity against human Hs 578T cells after 4 days by alamar blue assay, IC50=13.1μM |
21174434 |
HCT116 |
Antiproliferative assay |
|
72 hrs |
|
Antiproliferative activity against human HCT116 cells after 72 hrs by MTS assay, IC50=5.2μM |
22339433 |
MCF7 |
Antiproliferative assay |
|
72 hrs |
|
Antiproliferative activity against human MCF7 cells after 72 hrs by MTS assay, IC50=6.5μM |
22339433 |
A549 |
Antiproliferative assay |
|
72 hrs |
|
Antiproliferative activity against human A549 cells after 72 hrs by MTS assay, IC50=8.2μM |
22339433 |
LNCAP |
Cytotoxicity assay |
|
4 days |
|
Cytotoxicity against human LNCAP cells assessed as cell viability after 4 days by CCK8 method, IC50=4.59μM |
22832316 |
LNCAP |
Apoptosis assay |
>10 uM |
1 to 2 days |
|
Induction of apoptosis in human LNCAP cells assessed as increase in caspase3 activation at >10 uM after 1 to 2 days |
22832316 |
LNCAP |
Function assay |
10 uM |
12 to 24 hrs |
|
Inhibition of CK2alpha in human LNCAP cells assessed as reduction of survivin expression at 10 uM after 12 to 24 hrs by Western blot analysis |
22832316 |
LNCAP |
Function assay |
1 to 10 uM |
12 hrs |
|
Inhibition of CK2 in human LNCAP cells assessed as androgen receptor nuclear translocation at 1 to 10 uM after 12 hrs by Alexa Fluor 488 staining based fluorescence assay |
22832316 |
LNCAP |
Function assay |
1 to 10 uM |
1 day |
|
Inhibition of CK2 in human LNCAP cells assessed as DHT-mediated androgen receptor activation at 1 to 10 uM after 1 day by luciferase assay |
22832316 |
LNCAP |
Function assay |
10 uM |
8 hrs |
|
Inhibition of CK2 in human LNCAP cells assessed as reduction of androgen receptor dependent PSA mRNA level at 10 uM after 8 hrs by real time PCR analysis |
22832316 |
LNCAP |
Function assay |
10 uM |
8 hrs |
|
Inhibition of CK2 in human LNCAP cells assessed as reduction of androgen receptor dependent TMPRSS2 mRNA level at 10 uM after 8 hrs by real time PCR analysis |
22832316 |
LNCAP |
Function assay |
1 to 10 uM |
8 hrs |
|
Inhibition of CK2 in human LNCAP cells assessed as reduction of DHT-induced androgen receptor dependent TMPRSS2 mRNA expression at 1 to 10 uM after 8 hrs by real time PCR analysis |
22832316 |
LNCAP |
Function assay |
3 uM |
8 hrs |
|
Inhibition of CK2 in human LNCAP cells assessed as reduction of androgen receptor dependent DHT-induced PSA mRNA expression at 3 uM after 8 hrs by real time PCR analysis |
22832316 |
LNCAP |
Function assay |
10 uM |
12 to 24 hrs |
|
Inhibition of CK2 in human LNCAP cells assessed as reduction of androgen receptor nuclear translocation at 10 uM after 12 to 24 hrs by Western blot analysis |
22832316 |
LNCAP |
Apoptosis assay |
30 uM |
72 hrs |
|
Induction of apoptosis in human LNCAP cells at 30 uM after 72 hrs by AnnexinV staining based fluorescent microscopy |
22832316 |
LNCAP |
Function assay |
10 uM |
12 to 24 hrs |
|
Downregulation of CK2alpha expression in human LNCAP cells assessed as reduction of cytosolic protein level at 10 uM after 12 to 24 hrs by Western blot analysis |
22832316 |
LNCAP |
Function assay |
10 uM |
12 to 24 hrs |
|
Downregulation of CK2alpha expression in human LNCAP cells assessed as reduction of nuclear protein level at 10 uM after 12 to 24 hrs by Western blot analysis |
22832316 |
LNCAP |
Function assay |
10 uM |
12 to 24 hrs |
|
Inhibition of CK2alpha in human LNCAP cells assessed as reduction of p21 phosphorylation at threonine145 at 10 uM after 12 to 24 hrs by Western blot analysis |
22832316 |
LNCAP |
Function assay |
10 uM |
12 to 24 hrs |
|
Inhibition of CK2alpha in human LNCAP cells assessed as reduction of Akt phosphorylation at Ser473 at 10 uM after 12 to 24 hrs by Western blot analysis |
22832316 |
LNCAP |
Function assay |
10 uM |
12 to 24 hrs |
|
Inhibition of CK2alpha in human LNCAP cells assessed as reduction of XIAP expression at 10 uM after 12 to 24 hrs by Western blot analysis |
22832316 |
LNCAP |
Function assay |
10 uM |
12 to 24 hrs |
|
Inhibition of CK2alpha in human LNCAP cells assessed as induction of CHOP expression at 10 uM after 12 to 24 hrs by Western blot analysis |
22832316 |
LNCAP |
Function assay |
10 uM |
12 to 24 hrs |
|
Inhibition of CK2 in human LNCAP cells assessed as reduction of androgen receptor nuclear translocation at 10 uM after 12 to 24 hrs by HCS analysis |
22832316 |
MV4-11 |
Antiproliferative assay |
|
1 to 3 days |
|
Antiproliferative activity against human MV4-11 cells after 1 to 3 days by MTS assay, CC50=3μM |
23711832 |
U937 |
Antiproliferative assay |
|
1 to 3 days |
|
Antiproliferative activity against human U937 cells after 1 to 3 days by MTS assay, CC50=4.2μM |
23711832 |
Jurkat |
Antiproliferative assay |
|
1 to 3 days |
|
Antiproliferative activity against human Jurkat cells after 1 to 3 days by MTS assay, CC50=4.5μM |
23711832 |
K562 |
Antiproliferative assay |
|
1 to 3 days |
|
Antiproliferative activity against human K562 cells after 1 to 3 days by MTS assay, CC50=7μM |
23711832 |
A549 |
Function assay |
10 uM |
|
|
Inhibition of CK2-mediated ERK phosphorylation in human A549 cells at 10 uM by Western blot method |
24012124 |
A549 |
Function assay |
30 uM |
48 hrs |
|
Inhibition of CK2-mediated MMP2 activation in human A549 cells at 30 uM after 48 hrs by gelatin-zymography |
24012124 |
A549 |
Function assay |
10 uM |
4 to 24 hrs |
|
Inhibition of CK2-mediated AKT phosphorylation in human A549 cells at 10 uM after 4 to 24 hrs by Western blot method |
24012124 |
A549 |
Function assay |
10 uM |
15 to 30 mins |
|
Inhibition of CK2-mediated ERK phosphorylation in human A549 cells at 10 uM after 15 to 30 mins by Western blot method |
24012124 |
A549 |
Function assay |
10 uM |
24 hrs |
|
Inhibition of CK2-mediated AKT phosphorylation in human A549 cells at 10 uM after 24 hrs by Western blot method |
24012124 |
A549 |
Function assay |
1 to 10 uM |
24 hrs |
|
Inhibition of CK2-mediated MT1-MMP expression in human A549 cells at 1 to 10 uM after 24 hrs by Western blot method |
24012124 |
Sf9 |
Function assay |
|
|
|
Inhibition of CDK2/cyclin E (unknown origin) expressed in Sf9 cells using histone H1 as substrate in presence of [gamma33P]ATP, IC50=1.8μM |
24681986 |
A549 |
Cytotoxicity assay |
|
72 hrs |
|
Cytotoxicity against human A549 cells after 72 hrs by MTS assay, CC50=9.9μM |
26850376 |
DAOY |
qHTS assay |
|
|
|
qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for DAOY cells |
29435139 |
SJ-GBM2 |
qHTS assay |
|
|
|
qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for SJ-GBM2 cells |
29435139 |
A673 |
qHTS assay |
|
|
|
qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for A673 cells |
29435139 |
SK-N-MC |
qHTS assay |
|
|
|
qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for SK-N-MC cells |
29435139 |
BT-37 |
qHTS assay |
|
|
|
qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for BT-37 cells |
29435139 |
NB-EBc1 |
qHTS assay |
|
|
|
qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for NB-EBc1 cells |
29435139 |
U-2 OS |
qHTS assay |
|
|
|
qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for U-2 OS cells |
29435139 |
Saos-2 |
qHTS assay |
|
|
|
qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for Saos-2 cells |
29435139 |
SK-N-SH |
qHTS assay |
|
|
|
qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for SK-N-SH cells |
29435139 |
NB1643 |
qHTS assay |
|
|
|
qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for NB1643 cells |
29435139 |
LAN-5 |
qHTS assay |
|
|
|
qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for LAN-5 cells |
29435139 |
BT-12 |
qHTS assay |
|
|
|
qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for BT-12 cells |
29435139 |
Rh18 |
qHTS assay |
|
|
|
qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for Rh18 cells |
29435139 |
OHS-50 |
qHTS assay |
|
|
|
qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for OHS-50 cells |
29435139 |
RD |
qHTS assay |
|
|
|
qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for RD cells |
29435139 |
MG 63 (6-TG R) |
qHTS assay |
|
|
|
qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for MG 63 (6-TG R) cells |
29435139 |
Rh41 |
qHTS assay |
|
|
|
qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for Rh41 cells |
29435139 |
Sf21 insect |
Function assay |
|
20 mins |
|
Inhibition of recombinant human full length N-terminal His6-tagged CK2alpha expressed in Sf21 insect cells using CK2tide as substrate treated for 20 mins measured after 90 mins in presence of MgCl2 by caliper mobility shift assay, IC50<0.003μM |
29559278 |
786-0 |
Function assay |
|
24 hrs |
|
Inhibition of CK2 in human 786-0 cells assessed as decrease in Akt1 phosphorylation at Ser129 after 24 hrs by Western blot analysis, IC50=1μM |
30689946 |
786-0 |
Function assay |
|
24 hrs |
|
Inhibition of CK2 in human 786-0 cells assessed as decrease in alpha-catenin phosphorylation at Ser641 after 24 hrs by Western blot analysis, EC50=1μM |
30689946 |
786-0 |
Function assay |
|
24 hrs |
|
Inhibition of CK2 in human 786-0 cells assessed as reduction in STAT3 phosphorylation at Y705 after 24 hrs by Western blot analysis, EC50=5.3μM |
30689946 |
HeLa |
Function assay |
8 uM |
12 hrs |
|
Inhibition EGFP-CK2alpha (unknown origin) expressed in human HeLa cells induction of EGFP-CK2alpha translocation from nucleoli to nuclear matrix and cytoplasm at 8 uM after 12 hrs by Hoechst-33342-staining based microscopy |
30689946 |
Vero E6 |
Function assay |
|
48 hrs |
|
IC50 for antiviral activity against SARS-CoV-2 in the Vero E6 cell line at 48 h by immunofluorescence-based assay (detecting the viral NP protein in the nucleus of the Vero E6 cells), IC50=3.89045μM |
32353859 |
pulmonary smooth muscle cells |
Function assay |
|
|
|
Inhibition of de-differentiation of human pulmonary smooth muscle cells assessed as reduction in contractile to synthetic forms change, EC=0.001μM |
ChEMBL |
Jurkat T |
Function assay |
|
|
|
Modulation of Endogenous Assay: The human leukemia Jurkat T-cell line was maintained in RPMI 1640 (Cambrex) supplemented with 10% fetal calf serum and 50 ng/ml Geutamycin. Before treatment cells were washed, resuspended at a density of about 106 cells/mil, IC50=0.1μM |
ChEMBL |
pulmonary smooth muscle cells |
Function assay |
1 nM |
|
|
Inhibition of casein kinase-2/Nkx2-5 in human pulmonary smooth muscle cells assessed as down-regulation of COL1 protein expression at 1 nM by Western blot analysis |
ChEMBL |
pulmonary smooth muscle cells |
Function assay |
10 nM |
|
|
Inhibition of casein kinase-2/Nkx2-5 in human pulmonary smooth muscle cells assessed as down-regulation of COL1 protein expression at 10 nM by Western blot analysis |
ChEMBL |
pulmonary smooth muscle cells |
Function assay |
1 nM |
|
|
Inhibition of casein kinase-2/Nkx2-5 in human pulmonary smooth muscle cells assessed as down-regulation of CCN2 protein expression at 1 nM by Western blot analysis |
ChEMBL |
pulmonary smooth muscle cells |
Function assay |
10 nM |
|
|
Inhibition of casein kinase-2/Nkx2-5 in human pulmonary smooth muscle cells assessed as down-regulation of CCN2 protein expression at 10 nM by Western blot analysis |
ChEMBL |
pulmonary smooth muscle cells |
Function assay |
1 nM |
|
|
Inhibition of casein kinase-2/Nkx2-5 in human pulmonary smooth muscle cells assessed as up-regulation of ACTA2 protein expression at 1 nM by Western blot analysis |
ChEMBL |
pulmonary smooth muscle cells |
Function assay |
10 nM |
|
|
Inhibition of casein kinase-2/Nkx2-5 in human pulmonary smooth muscle cells assessed as up-regulation of ACTA2 protein expression at 10 nM by Western blot analysis |
ChEMBL |
pulmonary smooth muscle cells |
Function assay |
1 nM |
|
|
Inhibition of casein kinase-2/Nkx2-5 in human pulmonary smooth muscle cells assessed as up-regulation of MYH11 protein expression at 1 nM by Western blot analysis |
ChEMBL |
pulmonary smooth muscle cells |
Function assay |
10 nM |
|
|
Inhibition of casein kinase-2/Nkx2-5 in human pulmonary smooth muscle cells assessed as up-regulation of MYH11 protein expression at 10 nM by Western blot analysis |
ChEMBL |