Home Metabolism Casein Kinase inhibitor TBB For research use only.

TBB

Synonyms: 4,5,6,7-tetrabromobenzotriazole

TBB (4,5,6,7-tetrabromobenzotriazole) is a selective cell-permeable CK2 inhibitor with IC50 values of 0.9 and 1.6 μM for rat liver and human recombinant CK2 respectively.

TBB Chemical Structure

TBB Chemical Structure

CAS No. 17374-26-4

Purity & Quality Control

Batch: S526501 DMSO]86 mg/mL]false]]]false]]]false Purity: 99.68%
99.68

TBB Related Products

Biological Activity

Description TBB (4,5,6,7-tetrabromobenzotriazole) is a selective cell-permeable CK2 inhibitor with IC50 values of 0.9 and 1.6 μM for rat liver and human recombinant CK2 respectively.
Targets
CK2 [1]
(Cell-free assay)		 CK1 [1]
(Cel-free assay)
0.4 μM(Ki) 47 μM(Ki)
In vitro
In vitro In the presence of 10 μM TBB (and 100 μM ATP) only CK2 was drastically inhibited (s85%) whereas three kinases (phosphorylase kinase, glycogen synthase kinase 3L and cyclin-dependent kinase 2/cyclin A) underwent moderate inhibition, with IC50 values one-two orders of magnitude higher than CK2 (IC50 = 0.9 μM). TBB discriminates between CK2 and CK1, being much more e¡ective with the former[1].
Cell Research Cell lines Jurkat cells
Concentrations 60 μM
Incubation Time 2 h
Method

Cell treatment was performed by incubation of cell suspension (~10×106 cells/ml) for 2 h at 37℃ in the presence of 2 μM okadaic acid (OA) with the addition of protein kinase inhibitors where indicated. After incubations, cells were centrifuged and lysed for 15 min in ice-cold buffer consisting of 20 mM Tris^HCl pH 7.4, 2 mM EDTA, 2 mM EGTA, 10 mM 2-mercaptoethanol, 10% (v/v) glycerol, 1 mM Na3VO4, 0.05% Nonidet P-40, protease inhibitor cocktail, 10 mM NaF, 1 μM OA. The lysate was cleared by centrifugation for 10 min at 14 000 rpm.
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT02812290 Recruiting
Lung Transplant Rejection
University of Alberta
 May 2016 --
NCT02235779 Unknown status
Interstitial Lung Disease
Laval University
 August 2014 Not Applicable
NCT00888212 Completed
Sarcoidosis
University Hospital Ghent
 June 2008 Not Applicable

Chemical Information & Solubility

Molecular Weight 434.71 Formula

C6HBr4N3
CAS No. 17374-26-4 SDF --
Smiles C1(=C(C2=NNN=C2C(=C1Br)Br)Br)Br
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 86 mg/mL ( (197.83 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)


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In vivo
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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