Home Proteases MMP inhibitor SB-3CT For research use only.

SB-3CT

SB-3CT is an effective and selective gelatinase inhibitor with Ki of 13.9 nM and 600 nM for MMP-2 and MMP-9, respectively.

SB-3CT Chemical Structure

SB-3CT Chemical Structure

CAS No. 292605-14-2

Purity & Quality Control

SB-3CT Related Products

Signaling Pathway

MMP Signaling Pathways

MMP Signaling Pathway

Cell Data

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
A673 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for A673 cells 29435139
Saos-2 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for Saos-2 cells 29435139
BT-37 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for BT-37 cells 29435139
SK-N-SH qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for SK-N-SH cells 29435139
NB1643 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for NB1643 cells 29435139
OHS-50 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for OHS-50 cells 29435139
Rh41 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for Rh41 cells 29435139
SJ-GBM2 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for SJ-GBM2 cells 29435139
NB-EBc1 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for NB-EBc1 cells 29435139
LAN-5 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for LAN-5 cells 29435139
Click to View More Cell Line Experimental Data

Biological Activity

Description SB-3CT is an effective and selective gelatinase inhibitor with Ki of 13.9 nM and 600 nM for MMP-2 and MMP-9, respectively.
Targets
MMP-2 [1]
(Cell-free assay)		 MMP-9 [1]
(Cell-free assay)
13.9 nM(Ki) 600 nM(Ki)
In vitro
In vitro

SB-3CT directly inhibits bone marrow endothelial cell invasion and tubule formation in Matrigel in vitro. [3]
Kinase Assay Fluorescence enzymatic activity assays
The enzymatic activity of MMP-2, MMP-9 and MMP-7 is monitored with the fluorescence quenched substrate MOCAcPLGLA2pr(Dnp)-AR-NH2. Fluorescence is measure with a PTI spectrofluorometer. The cuvette compartment is thermostated at 25 ℃ 。
Cell Research Cell lines HCC cells
Concentrations 0.1 μM
Incubation Time 24 h
Method

Cells were treated with indicated concentration of drug for 24 h.
Experimental Result Images Methods Biomarkers Images PMID
Western blot Claudin11 / Occludin / ZO1 / Laminin-γ3 / β1-integrin 19828778
In Vivo
In vivo

In L-CI.5s T-cell lymphoma model, SB-3CT (5-50 mg/kg/d i.p.) potently inhibit liver metastasis and increases survival of mice. [2]

SB-3CT (50 mg/kg/d i.p.) inhibits human prostate cancer growth, osteolysis and angiogenesis in a bone metastasis model. [3]

In a mouse model of embolus-induced “permanent” focal cerebral ischemia, SB-3CT counteracts degradation of neuronal laminin, protects neurons from ischemic cell death, and ameliorates neurobehavioral outcomes after embolic MCA occlusion. [4]
Animal Research Animal Models Mouse L-CI.5s T-cell lymphoma model
Dosages 50 mg/kg/d
Administration i.p.

Chemical Information & Solubility

Molecular Weight 306.40 Formula

C15H14O3S2
CAS No. 292605-14-2 SDF Download SB-3CT SDF
Smiles C1C(S1)CS(=O)(=O)C2=CC=C(C=C2)OC3=CC=CC=C3
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 61 mg/mL ( (199.08 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 10 mg/mL

Water : Insoluble


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In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

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In vivo Formulation Calculator (Clear solution)

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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