Home Proteases MMP inhibitor Batimastat (BB-94) For research use only.

Batimastat (BB-94)

Batimastat (BB-94) is a potent, broad spectrum matrix metalloprotease (MMP) inhibitor for MMP-1, MMP-2, MMP-9, MMP-7 and MMP-3 with IC50 of 3 nM, 4 nM, 4 nM, 6 nM and 20 nM, respectively. Also inhibits the activitity of other metalloproteases, such as ADAM17.

Batimastat (BB-94) Chemical Structure

Batimastat (BB-94) Chemical Structure

CAS No. 130370-60-4

Purity & Quality Control

Batimastat (BB-94) Related Products

Signaling Pathway

MMP Signaling Pathways

MMP Signaling Pathway

Cell Data

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
Sf9 insect cells Function assay Inhibition of human matrix metalloprotease-2 expressed in Sf9 insect cells, IC50=0.013 μM 15582436
Sf9 insect cells Function assay Inhibition of human matrix metalloprotease-9 expressed in Sf9 insect cells, IC50=0.025 μM 15582436
HT1080 Function assay In vitro inhibitory activity against matrix metalloprotease 2 isolated from human HT1080 fibrosarcoma cells induced with TNF, IC50=0.0021μM 9873712
white blood cells Antifibrotic assay 25 to 150 mg/kg 13 days Antifibrotic activity in bleomycin-induced C57B1/6 mouse model assessed as inhibition of white blood cells in bronchoalveolar lavage at 25 to 150 mg/kg, po bid administered 2 hrs prior bleomycin induction for 13 days 15582436
RPM18866 Function assay Tested for inhibition against CD23 (IgE receptor) proteolysis in membranes derived from RPM18866 cells ( a human B-cell line), IC50=0.1μM 9871622
RPM18866 Function assay Inhibition against CD23 (IgE receptor) proteolysis in membranes derived from RPM18866 cells, IC50=0.1μM 9871623
Click to View More Cell Line Experimental Data

Biological Activity

Description Batimastat (BB-94) is a potent, broad spectrum matrix metalloprotease (MMP) inhibitor for MMP-1, MMP-2, MMP-9, MMP-7 and MMP-3 with IC50 of 3 nM, 4 nM, 4 nM, 6 nM and 20 nM, respectively. Also inhibits the activitity of other metalloproteases, such as ADAM17.
Targets
MMP-1 [1] MMP-2 [1] MMP-9 [1] MMP-7 [1] MMP-3 [1]
3 nM 4 nM 4 nM 6 nM 20 nM
In vitro
In vitro

Batimastat (BB-94) is a potent, broad spectrum matrix metalloprotease (MMP) inhibitor for MMP-1, MMP-2, MMP-9, MMP-7 and MMP-3 with IC50 of 3 nM, 4 nM, 4 nM, 6 nM and 20 nM, respectively. [1]

Batimastat exhibits an unexpected binding geometry, with the thiophene ring deeply inserted into the primary specificity site. [2]
Kinase Assay Enzyme assays
IC50s are determined for batimastat in vitro against different metalloproteinases in enzyme assays.
Cell Research Cell lines B16-BL6 cells
Concentrations 3 nM (MMP-1), 4 nM (MMP-2 & MMP-9), 6 nM (MMP-7) and 20 nM (MMP-3), respectively
Incubation Time 22 h
Method

Cells were treated with different concentrations of batimastat was dissolved in absolute ethanol and were incubated for 22 hr and IC50 was measured.
In Vivo
In vivo

Batimastat can inhibit metastatic spread and growth of B16-BL6 murine melanoma. [1]

In an orthotopic colon tumor model in mice, timastat treatment results in inhibition of primary tumor growth (by 50%), local/regional spread(from 67% to 35%), and distant metastasis(from 30% to 10%).[3]

Batimastat reduces in vivo growth of experimental hemangiomas, most probably by blocking endothelial cell recruitment by the transformed cells or by interfering with cell organization in vascular structures. [4]
Animal Research Animal Models Nude mouse
Dosages 30mg/ml
Administration i.p.

Chemical Information & Solubility

Molecular Weight 477.64 Formula

C23H31N3O4S2
CAS No. 130370-60-4 SDF Download Batimastat (BB-94) SDF
Smiles CC(C)CC(C(CSC1=CC=CS1)C(=O)NO)C(=O)NC(CC2=CC=CC=C2)C(=O)NC
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 96 mg/mL ( (200.98 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
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In vivo Formulation Calculator

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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