Home Cytoskeletal Signaling PKC inhibitor PKC-theta inhibitor For research use only.

PKC-theta inhibitor

PKC-theta inhibitor (compound 20) inhibits PKC-θ with an IC50 of 18 nM.

PKC-theta inhibitor Chemical Structure

PKC-theta inhibitor Chemical Structure

CAS No. 736048-65-0

Purity & Quality Control

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Signaling Pathway

PKC Signaling Pathways

PKC Signaling Pathway

Biological Activity

Description PKC-theta inhibitor (compound 20) inhibits PKC-θ with an IC50 of 18 nM.
Targets
PKCθ [1]
18 nM
In vitro
In vitro

PKC-theta inhibitor (compound 20) inhibits PKC-θ.
Cell Research Cell lines MN9D cells
Concentrations 5 μM
Incubation Time 24 h
Method

PKCθ inhibitor was dissolved in DMSO at 5 mM and diluted to the working concentration (5 μM) and cells were incubated with PKCθ inhibitor was 24 h.
In Vivo
In vivo

Compound 20 is a potent inhibitor of Protein Kinase C θ (PKC-θ). It improves muscle performance in the mdx mice model of Duchenne muscular dystrophy.
Animal Research Animal Models C57BL/6 mice
Dosages 10 mg/kg
Administration i.p.

Chemical Information & Solubility

Molecular Weight 454.45 Formula

C20H25F3N6O3
CAS No. 736048-65-0 SDF --
Smiles C1CC(CCC1CN)CNC2=NC(=NC=C2[N+](=O)[O-])NCC3=CC=CC=C3OC(F)(F)F
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 91 mg/mL ( (200.24 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 5 mg/mL

Water : Insoluble


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In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

Preparing Stock Solutions

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Mass Concentration Volume Molecular Weight

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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